Mouse Anti-PPIB Recombinant Antibody (clone 2B10) (CAT#: ZG-0208J)
This product is a mouse antibody that recognizes PPIB.
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Custom Production
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IF
Figure 1 Mouse Anti-PPIB Recombinant Antibody (clone 2B10) in IF
Immunofluorescence analysis of Hela cell. 1.Calmodulin Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min).
IF
Figure 2 Mouse Anti-PPIB Recombinant Antibody (clone 2B10) in IF
Immunofluorescence analysis of human-lung tissue. 1.Cyclophilin B Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary Antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI.Picture C: merge of A+B
IHC
Figure 3 Mouse Anti-PPIB Recombinant Antibody (clone 2B10) in IHC
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1.Cyclophilin B Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min). 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary Antibody only.
IHC
Figure 4 Mouse Anti-PPIB Recombinant Antibody (clone 2B10) in IHC
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1.Cyclophilin B Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min). 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary Antibody only.
WB
Figure 5 Mouse Anti-PPIB Recombinant Antibody (clone 2B10) in WB
Western blot analysis of 1) Jurkat, 2) 293T, 3) Rat Liver Tissue, 4) 3T3 with Cyclophilin B Mouse mAb diluted at 1:2,000.
Specifications
- Immunogen
- Synthetic Peptide of Cyclophilin B
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human PPIB
- Species Reactivity
- Human, Rat, Mouse
- Clone
- 2B10
- Applications
- IF/ICC, WB, IHC-P
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Immunofluorescence/Immunocytochemistry, Western Blot, Immunohistochemistry-Paraffin.
Target
- Alternative Names
- OI9; CYPB; SCYLP; CYP-S1; HEL-S-39; peptidyl-prolyl cis-trans isomerase B; PPIase B; S-cyclophilin; cyclophilin-like protein; epididymis secretory protein Li 39; peptidylprolyl isomerase B (cyclophilin B); rotamase B; Cyclophilin B
- Gene ID
- 5479
- UniProt ID
- P23284
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2805 | Hi-Affi™ Recombinant Rabbit Anti-PPIB Monoclonal Antibody (DS2805AB) | WB | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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