Mouse Anti-PPIB Recombinant Antibody (clone 2B10) (CAT#: ZG-0208J)
This product is a mouse antibody that recognizes PPIB.
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Custom Production
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IF
Figure 1 Mouse Anti-PPIB Recombinant Antibody (clone 2B10) in IF
Immunofluorescence analysis of Hela cell. 1.Calmodulin Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min).
IF
Figure 2 Mouse Anti-PPIB Recombinant Antibody (clone 2B10) in IF
Immunofluorescence analysis of human-lung tissue. 1.Cyclophilin B Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary Antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI.Picture C: merge of A+B
IHC
Figure 3 Mouse Anti-PPIB Recombinant Antibody (clone 2B10) in IHC
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1.Cyclophilin B Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min). 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary Antibody only.
IHC
Figure 4 Mouse Anti-PPIB Recombinant Antibody (clone 2B10) in IHC
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1.Cyclophilin B Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min). 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary Antibody only.
WB
Figure 5 Mouse Anti-PPIB Recombinant Antibody (clone 2B10) in WB
Western blot analysis of 1) Jurkat, 2) 293T, 3) Rat Liver Tissue, 4) 3T3 with Cyclophilin B Mouse mAb diluted at 1:2,000.
Specifications
- Immunogen
- Synthetic Peptide of Cyclophilin B
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human PPIB
- Species Reactivity
- Human, Rat, Mouse
- Clone
- 2B10
- Applications
- IF/ICC, WB, IHC-P
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Immunofluorescence/Immunocytochemistry, Western Blot, Immunohistochemistry-Paraffin.
Target
- Alternative Names
- OI9; CYPB; SCYLP; CYP-S1; HEL-S-39; peptidyl-prolyl cis-trans isomerase B; PPIase B; S-cyclophilin; cyclophilin-like protein; epididymis secretory protein Li 39; peptidylprolyl isomerase B (cyclophilin B); rotamase B; Cyclophilin B
- Gene ID
- 5479
- UniProt ID
- P23284
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2805 | Hi-Affi™ Recombinant Rabbit Anti-PPIB Monoclonal Antibody (DS2805AB) | WB | IgG |
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For Research Use Only. Not For Clinical Use.
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