Rabbit Anti-PSMA6 Recombinant Antibody (clone R01-4I2)
CAT#: VS3-FY1855
Clone R01-4I2 is a rabbit recombinant monoclonal antibody that exhibits specific binding to human PSMA6. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, ICC/IF. This proteasome alpha 6 antibody was verified by western blot analysis of proteasome subunit alpha type 6 in K562, C6, 3T3, Hela lysates.
Tested Data
WB
Figure 1 Anti-PSMA6 Antibody (VS3-FY1855) in WB.
The Proteasome subunit alpha type 6 expression in K562, C6, 3T3, Hela lysates were detected using the anti-Proteasome alpha 6 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human proteasome alpha subunit type 6
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PSMA6
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-4I2
- Applications
- Western Blot, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 27 kDa, while the observed molecular weight stands at 27 kDa.
- Related Disease
- Myocardial Infarction and Combined Oxidative Phosphorylation Deficiency 8
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- IOTA; p27K; PROS27
- Gene ID
- 5687
- UniProt ID
- P60900
- Sequence Similarities
- Belongs to the peptidase T1A family.
- Cellular Localization
- Cytoplasm, Nucleus, Proteasome
- Post Translation Modifications
- Glycosylation at Ser5 (NX_P60900 [NX_P60900-1])
Ubiquitination at Lys102, Lys104, Lys45, Lys30, Lys55, Lys116, Lys59, Lys164, Lys171, and Lys181 (NX_P60900 [NX_P60900-1])
Modification sites at PhosphoSitePlus (P60900)
Glycosylation from GlyGen (P60900) 2 sites, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001269161.1; NP_001269162.1; NP_001269163.1; NP_002782.1
- Function
- The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PSMA6"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3033z | Mouse Anti-PSMA6 Recombinant Antibody (clone 1B12) | WB, IHC | Mouse IgG1 |
| VS13-YC950 | Rabbit Anti-PSMA6 Recombinant Antibody (VS13-YC950) | WB, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2891 | Hi-Affi™ Recombinant Rabbit Anti-PSMA6 Monoclonal Antibody (DS2891AB) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY418 | Mouse Anti-NHP PSMA6 Recombinant Antibody (clone OTI4C9) | WB, IHC, FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1780 | Anti-PSMA6 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5778 | Anti-Mouse PSMA6 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5777 | Anti-Human PSMA6 Immunohistochemistry Kit | IHC |
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