Human Anti-PTx Recombinant Antibody (clone A12) (CAT#: HPAB-A1287-YJ)

Figure 1 ELISA was used to assess the binding of the monoclonal antibodies to native PTx and genetically modified PTx (PTg).

The ELISAs were performed at least twice with similar results.

Figure 2 Binding of the antibodies to purified PTx-S1 on a western immunoblot.

Figure 3 Anti-PTx antibody binding to whole cell B. pertussis monitored by flow cytometry.

Figure 4 Antibody-mediated blockade of PTx-receptor binding using fetuin as a model receptor.

Wells were coated with fetuin and blocked. Antibody hu11E6 is known to block PTx-receptor interactions was used as a positive control, while wells lacking PTx were used as a negative control.

Figure 5 Epitope binning of isolated antibodies by competition ELISA.

Results for each antibody pair are from at least one ELISA experiment performed with duplicate antibody samples.