Human Anti-TSST-1 Recombinant Antibody (clone MS473) (CAT#: VS-0323-CJ62)
This human anti-TSST-1 antibody (clone MS473) is a product that recognizes S. aureus TSST-1.
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ELISA
Figure 1 Screening of the scFv monoclonal phage ELISA MS473 on TSST-1.
A total of 20 phage clones with potential binding to TSST-1 protein were identified, VS-0323-CJ62 had the one of the highest binding signals.
ELISA
Figure 2 The binding ability of purified MS473 scFv to TSST-1 was assessed by ELISA.
The wells coated with TSST-1 or bovine serum albumin (BSA) (as control) were individually incubated with MS473 or SP220, followed by mouse anti-human scFv polyclonal antibody (MPAb) or unimmunized mouse sera (UnMsera; control). Empty wells were used as the control. Next, the wells were incubated with goat anti-mouse IgG-horseradish peroxidase (HRP)-conjugated antibody (GtAb), followed by the addition of the TMB solution.
ELISA
Figure 3 The binding affinity of MS473 and RA16 (an scFv specific to IL-6) to TSST-1 (1 and 2 µg/ml) was measured by non-competitive ELISA.
ELISA
Figure 4 The specific binding of MS473 to TSST-1 was evaluated by ELISA.
Inhib
Figure 5 Inhibition of the mitogenic response of human PBMCs to TSST-1 by VS-0323-CJ62.
VS-0323-CJ62 exhibited a significant inhibitory effect (the IC50 value: 1.5 µM) on the proliferation of PBMCs and decreased the number of colony-forming cells.
FuncS
Figure 6 Inhibition effects of MS473 on the production of cytokines from human PBMCs stimulated with TSST-1.
IA
Figure 7 Reactivity of MS473 to TSST-1 peptides involved in the TCR binding.
The MS473 scFv reacted significantly with the peptides T1 and T2, which are located in the region of TSST-1 that interacts with the human TCR Vβ-chain. There was no significant reaction between the MS473 scFv and peptides M1 and M2, both of which are located within the region of TSST-1 that interacts with the MHC class II.
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- S. aureus TSST-1
- Species Reactivity
- S. aureus
- Clone
- MS473
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Immunoassay, Inhibition, Functional Assay
- Conjugate
- Unconjugated
- Related Disease
- Toxic shock syndrome
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- ELISA: 2 ng/mL-5 ng/mL
Inhibition assay: 0.1 μM-4.7 μM
Target
- Alternative Names
- Toxic shock syndrome toxin-1
- UniProt ID
- P06886
- Sequence Similarities
- Belongs to the staphylococcal/streptococcal toxin family.
- Cellular Localization
- Secreted
- Function
- Responsible for the symptoms of toxic shock syndrome.
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY51 | Human Anti-TSST-1 (clone MS473) scFv-Fc Chimera | Inhib, ELISA | Human IgG1, scFv-Fc |
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For Research Use Only. Not For Clinical Use.
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