What is the recommended storage method and shelf life for the H-2Dd/HIV IIIB gp120 (RGPGRAFVTI) MHC Pentamer?

The shelf life is typically 6 months when stored at 4°C in the dark. For longer storage, aliquot and freeze at -80°C to maintain stability and activity.

Is it possible to use this pentamer in a multiplex experiment to find numerous antigen-specific T cells at once?

Yes, you can use it in multiplex assays as long as each pentamer is conjugated with a different fluorochrome to distinguish between multiple antigen-specific T cell populations.

When using fresh or frozen cells, is the H-2Dd/HIV IIIB gp120 (RGPGRAFVTI) MHC Pentamer compatible?

Yes, the pentamer is compatible with both fresh and frozen cells. However, ensure that frozen cells are properly thawed and viable before use.

H-2Dd/HIV IIIB gp120 (RGPGRAFVTI) MHC Pentamer (CAT#: MHC-YF497)

MHC pentamers have a high avidity for CD8 T cells, and can be used in the analysis of individual antigen-specific T cells. This product is a MHC pentamer of peptide/MHC complex composed IIIB gp120-derived peptide of RGPGRAFVTI sequence covering 318-327 and H-2Dd molecule. The peptide/MHC pentamer provided here is unlabelled or fluorescent labelled, such as Biotin, R-PE, APC. All products are available in 50, 150 and 500 tests.

Specific Inquiry

Custom Production

MHC tetramer custom production provides a vital tool for researchers seeking to understand and manipulate T-cell immunity with exceptional precision. Please specify your requirements, including peptide sequence, MHC allele, and desired fluorophore etc. We will respond as soon as possible with a tailored solution.

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Disclaimer: Please note that the MHC reagents we offered have been produced with strict quality control (including gene sequencing, affinity purification, SDS-PAGE analysis, and verification of biotinylation), but many have not been confirmed to stain or activate specific T cells.

We require custom production

Datasheet
MSDS
COA

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Allele
H-2Dd

Class
Class I

MHC Species
Mouse

Antigen
IIIB gp120

Antigen Species
HIV

Peptide
RGPGRAFVTI

Range
318-327

Conjugate
Unlabeled, Biotin, R-PE, APC

Application
FCM

Target

Antigen Introduction
Envelope glycoprotein gp160: Oligomerizes in the host endoplasmic reticulum into predominantly trimers. In a second time, gp160 transits in the host Golgi, where glycosylation is completed. The precursor is then proteolytically cleaved in the trans-Golgi and thereby activated by cellular furin or furin-like proteases to produce gp120 and gp41.

Alternative Names
Env polyprotein; env; Envelope glycoprotein gp160

Gene ID
155971

UniProt ID
A0A343R245

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CAT	Product Name	Application	Type
AGTO-L012R	anti-env immunotoxin 907 (IgG)-RTA	Cytotoxicity assay, Functional assay

CAT	Product Name	Application	Type
PFBL-193	Recombinant Human Anti-HIV-1 gp41-M-MAT Antibody Fab Fragment	Neut, FuncS	Fab
PSBZ-168	Recombinant Human Anti-HIV-1 gp120 Antibody scFv Fragment (PGT 135)	ELISA, Neut	scFv
PSBZ-169	Recombinant Human Anti-HIV-1 gp120 Antibody scFv Fragment (45-46M2)	ELISA, Neut	scFv
PSBZ-205	Recombinant Human Anti-HIV-1 gp120 Antibody scFv Fragment (PGT121)	Neut, ELISA	scFv
PSBW-153	Recombinant Human Anti-HIV-1 gp120 Antibody scFv Fragment (B13)	FACS, Neut	scFv

CAT	Product Name	Application	Type
PNBL-020	Recombinant Anti-HIV ENV VHH Single Domain Antibody (PNBL-020)	ELISA, SPR, Neut, FuncS	Llama VHH
PNBL-021	Recombinant Anti-HIV-1 ENV VHH Single Domain Antibody (PNBL-021)	Neut, WB, ELISA, FuncS	Llama VHH
PNBL-059	Recombinant Anti-HIV-1 gp120 VHH Single Domain Antibody (PNBL-059)	ELISA, Inhib	Llama VHH

CAT	Product Name	Application	Type
PABZ-160	Recombinant Human Anti-HIV-1 gp120 Antibody (PGT123)	FACS, Neut	IgG
PABZ-161	Recombinant Human Anti-HIV-1 gp120 Antibody (PGT122)	FACS, Neut	IgG
PABZ-168	Recombinant Human Anti-HIV-1 gp120 Antibody (PGT 135)	ELISA, Neut	IgG
PABZ-169	Recombinant Human Anti-HIV-1 gp120 Antibody (45-46M2)	ELISA, Neut	IgG
PABZ-199	Recombinant Human Anti-HIV-1 gp120 Antibody (PGT121-GL)	Neut, ELISA	IgG

CAT	Product Name	Application	Type
PFBZ-141	Human Anti-ENV Recombinant Antibody (clone 2F5); Fab Fragment	Neut	Human Fab
PFBZ-160	Recombinant Human Anti-HIV-1 gp120 Antibody Fab Fragment (PGT123)	FACS, Neut	Fab
PFBZ-161	Recombinant Human Anti-HIV-1 gp120 Antibody Fab Fragment (PGT122)	FACS, Neut	Fab
PFBZ-168	Recombinant Human Anti-HIV-1 gp120 Antibody Fab Fragment (PGT 135)	ELISA, Neut	Fab
PFBZ-169	Recombinant Human Anti-HIV-1 gp120 Antibody Fab Fragment (45-46M2)	ELISA, Neut	Fab

CAT	Product Name	Application
MHC-YF188	H-2Db/LCMV gp (KAVYNFATC) MHC Monomer	MHC Multimer
MHC-YF410	A*02:01/HIV Env (SLLNATAIAV) MHC Pentamer	FCM
MHC-YF411	A*29:02/HIV Env (SFDPIPIHY) MHC Pentamer	FCM
MHC-YF412	A*29:02/HIV Env (SFNCRGEFFY) MHC Pentamer	FCM
MHC-YF413	B*08:01/HIV Env (YLKDQQLL) MHC Pentamer	FCM

Customer Reviews and Q&As

Customer Review Q&As

Amazing specificity and sensitivity

I was impressed with the specificity and sensitivity of this pentamer. It's been invaluable in my research on antigen-specific T cell responses to HIV, especially in post-vaccination studies.

Great tool for studying HIV vaccine

Great tool for tracking T cell responses in our HIV vaccine studies. It has enhanced our understanding of T cell dynamics and vaccine-induced immunity.

Satisfying customer support

I appreciate the customer support for this product. They provided excellent guidance on usage, which made a big difference in our experimental outcomes.

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