Human Anti-pSMAD3 scFv-Fc Chimera (VS-0325-FY200) (CAT#: VS-0325-FY200)
The anti-pSMAD3 scFv-Fc antibody targets phosphorylated SMAD3, a key protein in the TGF-β signaling pathway. This antibody combines scFv specificity with Fc stability, making it a valuable tool for studying SMAD3 phosphorylation, signal transduction, and its role in cell differentiation, tissue repair, and disease processes.
Specific Inquiry
Specifications
- Immunogen
- Peptides derived from SMAD3
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Specificity
- Human pSMAD3
- Species Reactivity
- Human
- Applications
- ELISA, Immunofluorescence
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- SMAD Family Member 3; Mothers Against DPP Homolog 3; MAD Homolog 3; JV15-2; HMAD-3; HSMAD3; MADH3; MAD3; MAD, Mothers Against Decapentaplegic Homolog 3 (Drosophila); SMAD, Mothers Against DPP Homolog 3 (Drosophila); MAD, Mothers Against Decapentaplegic Homolog 3; Mothers Against Decapentaplegic Homolog 3
- Gene ID
- 4088
- UniProt ID
- P84022
- Long Name
- SMAD Family Member 3
- Sequence Similarities
- Belongs to the dwarfin/SMAD family.
- Cellular Localization
- Cytoplasm; Nucleus
- Post Translation Modifications
- Phosphorylated on serine and threonine residues.
Ubiquitinated.
Monoubiquitinated, leading to prevent DNA-binding.
Deubiquitination by USP15 alleviates inhibition and promotes activation of TGF-beta target genes.
Ubiquitinated by RNF111, leading to its degradation: only SMAD3 proteins that are 'in use' are targeted by RNF111, RNF111 playing a key role in activating SMAD3 and regulating its turnover (By similarity).
Undergoes STUB1-mediated ubiquitination and degradation.
Ubiquitination at Lys33 and Lys81
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_054233889.1; XP_054233890.1; NP_001138574.1; NP_001138575.1; NP_001138576.1
- Function
- Human pSMAD3 refers to phosphorylated SMAD3, the activated form of SMAD3. Phosphorylation of SMAD3 is crucial for its signaling activity and its ability to regulate gene expression.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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