Recombinant Mouse Anti-NOS3 Antibody (VS3-FY500) (CAT#: VS3-FY500)
This product is a recombinant mouse antibody that recognizes human, mouse and rat NOS3. The eNOS antibody can detects endogenous eNOS protein protein. It can detect a band of approximately 130 kDa.
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Normal Tissue
RNA Expression
Figure 1 High expression in spleen.
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/ihc_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/ihc_selected.jpg
Figure 2 Low expression in cerebral cortex.
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/5989_B_7_5_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/5989_B_7_5_val_selected.jpg
Figure 3 Cerebral cortex
(Glial cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/5989_B_7_5.jpg
(Glial cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/5989_B_7_5.jpg
Figure 4 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/5989_A_7_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/5989_A_7_3.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/5989_A_9_5.jpg
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/5989_A_9_5.jpg
Figure 6 RNA cell line category: Cell line enhanced (BEWO, HUVEC TERT2, K-562, TIME)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164867-NOS3
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164867-NOS3
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- The eNOS antibody can detects endogenous eNOS protein.
- Species Reactivity
- Human, Mouse, Rat
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- 130 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, pH 7.4, 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western blotting: 1/1000-1/5000
Target
- Alternative Names
- Nitric Oxide Synthase 3; Nitric Oxide Synthase 3 (Endothelial Cell); Constitutive NOS; Endothelial NOS; EC 1.14.13.39; NOS Type III; EC-NOS; NOSIII; CNOS
- Gene ID
- 4846
- UniProt ID
- P29474
- Sequence Similarities
- Belongs to the NOS family.
- Cellular Localization
- Cell membrane, Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane
- Post Translation Modifications
- Phosphorylation by AMPK at Ser-1177 in the presence of Ca2+-calmodulin (CaM) activates activity. In absence of Ca2+-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity).
Phosphorylation of Ser-114 by CDK5 reduces activity.
- Protein Refseq
- NP_000594.2; NP_001153581.1; NP_001153582.1
- Function
- Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway (PubMed:1378832).
NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Recommended Products
Secondary Antibody
Recommended Dilution Buffer
Isotype Control
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibodies
IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2144z | Mouse Anti-NOS3 Recombinant Antibody (clone 25D5) | WB, ELISA, IF, IHC, IP | Mouse IgG2a, κ |
| MRO-1840-CN | Rabbit Anti-NOS3 Polyclonal Antibody (MRO-1840-CN) | IHC | Rabbit IgG |
| VS3-CJ858 | Mouse Anti-NOS3 Recombinant Antibody (VS3-CJ858) | WB, IHC | Mouse IgG |
| VS3-XY1172 | Mouse Anti-NOS3 Recombinant Antibody (clone 6H2) | ELISA, IHC | Mouse IgG1 |
| VS3-WK1419 | Mouse Anti-NOS3 Antibody (clone Mix-mA) | WB | Mouse IgG1 |
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