Mouse Anti-NOS3 Monoclonal Antibody (VS7-0425-WR489) (CAT#: VS7-0425-WR489)
This product is a mouse IgG antibody that binds to human NOS3. The antibody can be used for IHC-P, IF, and ELISA assays.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Normal Tissue
RNA Expression
Figure 1 High expression in spleen.
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/ihc_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/ihc_selected.jpg
Figure 2 Low expression in cerebral cortex.
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/5989_B_7_5_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/5989_B_7_5_val_selected.jpg
Figure 3 Cerebral cortex
(Glial cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/5989_B_7_5.jpg
(Glial cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/5989_B_7_5.jpg
Figure 4 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/5989_A_7_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/5989_A_7_3.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/5989_A_9_5.jpg
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/5989_A_9_5.jpg
Figure 6 RNA cell line category: Cell line enhanced (BEWO, HUVEC TERT2, K-562, TIME)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164867-NOS3
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164867-NOS3
Specifications
- Immunogen
- Recombinant human NOS3 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human NOS3
- Species Reactivity
- Human
- Molecule Class
- Nitric Oxide Synthase
- Applications
- IHC-P, IF, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Variation Asp-298 in NOS3 may be associated with susceptibility to coronary spasm
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Immunohistochemistry: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- NOS3; Nitric Oxide Synthase 3 Transcript Variant ENOS-Delta21; NOSIII; Nitric Oxide Synthase 3 Transcript Variant ENOS-Delta20; Nitric Oxide Synthase 3 (Endothelial Cell); Nitric Oxide Synthase 3 Transcript Variant ENOS-Delta20-21; ECNOS; Nitric Oxide Syn
- Gene ID
- 4846
- UniProt ID
- P29474
- Long Name
- Nitric oxide synthase 3
- Cellular Localization
- Cell membrane; Cytoplasm; Cytoskeleton; Golgi apparatus; Membrane
- Post Translation Modifications
- Lipoprotein; Myristate; Palmitate; Phosphoprotein
- Protein Refseq
- NP_000594.2
- Function
- Calmodulin-binding; Oxidoreductase
Recommended Products
Isotype Control
Secondary Antibody
Related Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "NOS3"
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2144z | Mouse Anti-NOS3 Recombinant Antibody (clone 25D5) | WB, ELISA, IF, IHC, IP | Mouse IgG2a, κ |
| ZG-0318C | Mouse Anti-NOS3 Recombinant Antibody (ZG-0318C) | IHC, ELISA | Mouse IgG |
| VS3-CJ858 | Mouse Anti-NOS3 Recombinant Antibody (VS3-CJ858) | WB, IHC | Mouse IgG |
| VS3-WK1419 | Mouse Anti-NOS3 Antibody (clone Mix-mA) | WB | Mouse IgG1 |
| VS3-FY500 | Recombinant Mouse Anti-NOS3 Antibody (VS3-FY500) | WB | Mouse IgG |
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