Recombinant Mouse Anti-NOS3 Antibody (VS4-CJ57) (CAT#: VS4-CJ57)
This mouse anti-NOS3 antibody is a product that recognizes human, mouse, rat NOS3. The immunogen of this antibody is a recombinant protein corresponding to NOS3. The mAb has been tested for use in ELISA, Western Blot (1:500-1:5000).
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Normal Tissue
RNA Expression
Figure 1 High expression in spleen.
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/ihc_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/ihc_selected.jpg
Figure 2 Low expression in cerebral cortex.
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/5989_B_7_5_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/5989_B_7_5_val_selected.jpg
Figure 3 Cerebral cortex
(Glial cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/5989_B_7_5.jpg
(Glial cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/5989_B_7_5.jpg
Figure 4 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/5989_A_7_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/5989_A_7_3.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/5989_A_9_5.jpg
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/5989_A_9_5.jpg
Figure 6 RNA cell line category: Cell line enhanced (BEWO, HUVEC TERT2, K-562, TIME)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164867-NOS3
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164867-NOS3
Specifications
- Immunogen
- Recombinant Protein
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, Mouse, Rat NOS3
- Species Reactivity
- Human, Mouse, Rat
- Applications
- ELISA, WB
- Conjugate
- Unconjugated
Product Property
- Purification
- Immunogen affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, PBS, pH 7.4.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Western Blot (1:500-1:5000).
Target
- Alternative Names
- eNOS; ECNOS
- Gene ID
- 4846
- UniProt ID
- P29474
- Long Name
- Nitric oxide synthase 3
- Sequence Similarities
- Belongs to the NOS family.
- Cellular Localization
- Cell membrane, Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane
- Post Translation Modifications
- Phosphorylation by AMPK at Ser-1177 in the presence of Ca2+-calmodulin (CaM) activates activity. In absence of Ca2+-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity).
Phosphorylation of Ser-114 by CDK5 reduces activity.
- Protein Refseq
- NP_000594.2; NP_001153581.1; NP_001153582.1
- Function
- Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway (PubMed:1378832).
NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2144z | Mouse Anti-NOS3 Recombinant Antibody (clone 25D5) | WB, ELISA, IF, IHC, IP | Mouse IgG2a, κ |
| MRO-1840-CN | Rabbit Anti-NOS3 Polyclonal Antibody (MRO-1840-CN) | IHC | Rabbit IgG |
| ZG-0318C | Mouse Anti-NOS3 Recombinant Antibody (ZG-0318C) | IHC, ELISA | Mouse IgG |
| VS3-XY1172 | Mouse Anti-NOS3 Recombinant Antibody (clone 6H2) | ELISA, IHC | Mouse IgG1 |
| VS7-0425-WR489 | Mouse Anti-NOS3 Monoclonal Antibody (VS7-0425-WR489) | IHC-P, IF, ELISA | Mouse IgG |
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