Mouse Anti-4-Hydroxy-3-nitrophenylacetic acid Recombinant Antibody (clone BBE6.12H3); Fab Fragment (CAT#: PFBC-001)
Recombinant Mouse Antibody (BBE6. 12H3) Fab Fragment is specific to (4-hydroxy-3-nitrophenyl)-acetate (NP).
Specific Inquiry
Figure 1 Cross-reactive properties of mAbs 36-65 and BBE6.12H3.
A, Distribution of Kd values obtained for either Ab in Tables I and II, respectively. B and C, Relative contribution from ΔHeq (•) and TΔSeq (○) to the overall free energy of binding (ΔGeq, crosses) for each of the phage clones cross-reactive with either mAb 36-65 (B) or mAb BBE6.12H3 (C). These values were calculated from the mean kass and kdiss values of two separate experiments, performed on independently immobilized cuvettes, as described in Results. For both panels, the order of presentation from left to right is that of the phage clones listed from top to bottom in Tables I and II. The inset in both panels gives the distribution of TΔSass obtained.
Manivel, V., Bayiroglu, F., Siddiqui, Z., Salunke, D. M., & Rao, K. V. (2002). The primary antibody repertoire represents a linked network of degenerate antigen specificities. The Journal of Immunology, 169(2), 888-897.
Figure 2 Temperature Sensitivity of Affinity of Germline and Mutated Antibodies for Antigen
Manivel, V., Sahoo, N. C., Salunke, D. M., & Rao, K. V. (2000). Maturation of an antibody response is governed by modulations in flexibility of the antigen-combining site. Immunity, 13(5), 611-620.
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG
- Specificity
- Tested positive against synthetic (4-hydroxy-3-nitrophenyl)-acetate (NP)
- Clone
- BBE6.12H3
- Applications
- WB, ELISA
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- 4-Hydroxy-3-nitrophenylacetic acid; 10463-20-4; 2-(4-HYDROXY-3-NITROPHENYL)ACETIC ACID; (4-hydroxy-3-nitrophenyl)acetic acid; 3-Nitro-4-hydroxy phenylacetic acid; CHEBI:546274
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
Fab Fragment Antibody
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-10 | Recombinant Anti-4-hydroxy-3-nitrophenylacetic acid Antibody | WB, FC, FuncS | IgG |
| MHH-10 | Recombinant Human Anti-4-hydroxy-3-nitrophenylacetic acid Antibody | WB, FACS, FuncS | IgG |
| PABC-001 | Mouse Anti-4-Hydroxy-3-nitrophenylacetic acid Recombinant Antibody (clone BBE6.12H3) | WB, ELISA | Mouse IgG |
| PABW-004 | Mouse Anti-4-hydroxy-3-nitrophenylacetic acid Recombinant Antibody (clone 88C6/12) | WB, IHC, IF, FuncS | Mouse IgG1, λ |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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