Mouse Anti-4-Hydroxy-3-nitrophenylacetic acid Recombinant Antibody (clone BBE6.12H3); Fab Fragment (CAT#: PFBC-001)

Recombinant Mouse Antibody (BBE6. 12H3) Fab Fragment is specific to (4-hydroxy-3-nitrophenyl)-acetate (NP).

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  • Published Data
  • Datasheet
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Figure 1 Cross-reactive properties of mAbs 36-65 and BBE6.12H3.

Figure 1 Cross-reactive properties of mAbs 36-65 and BBE6.12H3.

A, Distribution of Kd values obtained for either Ab in Tables I and II, respectively. B and C, Relative contribution from ΔHeq (•) and TΔSeq (○) to the overall free energy of binding (ΔGeq, crosses) for each of the phage clones cross-reactive with either mAb 36-65 (B) or mAb BBE6.12H3 (C). These values were calculated from the mean kass and kdiss values of two separate experiments, performed on independently immobilized cuvettes, as described in Results. For both panels, the order of presentation from left to right is that of the phage clones listed from top to bottom in Tables I and II. The inset in both panels gives the distribution of TΔSass obtained.

Manivel, V., Bayiroglu, F., Siddiqui, Z., Salunke, D. M., & Rao, K. V. (2002). The primary antibody repertoire represents a linked network of degenerate antigen specificities. The Journal of Immunology, 169(2), 888-897.

Figure 2 Temperature Sensitivity of Affinity of Germline and Mutated Antibodies for Antigen

Figure 2 Temperature Sensitivity of Affinity of Germline and Mutated Antibodies for Antigen

Manivel, V., Sahoo, N. C., Salunke, D. M., & Rao, K. V. (2000). Maturation of an antibody response is governed by modulations in flexibility of the antigen-combining site. Immunity, 13(5), 611-620.


  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Tested positive against synthetic (4-hydroxy-3-nitrophenyl)-acetate (NP)
  • Clone
  • BBE6.12H3
  • Applications

Product Property

  • Purity
  • >95% as determined by analysis by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.


  • Alternative Names
  • 4-Hydroxy-3-nitrophenylacetic acid; 10463-20-4; 2-(4-HYDROXY-3-NITROPHENYL)ACETIC ACID; (4-hydroxy-3-nitrophenyl)acetic acid; 3-Nitro-4-hydroxy phenylacetic acid; CHEBI:546274

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.


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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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