Recombinant Mouse Anti-B. tropicalis Blo t 5 Antibody (4A7) (CAT#: PABZ-136)
Recombinant Mouse Antibody (4A7) is capable of binding to B. tropicalis Blo t 5, expressed in Chinese Hamster Ovary cells (CHO). The mAb epitope comprises two closely spaced surfaces, I and II, connected by a sharp turn and bearing critical residues Asn46 and Lys47 on one surface, and Lys54 and Arg57 on the other. This discontinuous epitope overlaps with the human IgE epitope(s) of Blo t 5.
Specific Inquiry
Figure 1 Development of two-site enzyme-linked immunosorbent assay (ELISA) using Blo t 5 mAb and Blo t 5 specific guinea pig immunoglobulins as capture and detection antibody, respectively.
The Blo t 5 two-site ELISA developed has high sensitivity and specificity. (A) Comparison of rBlo t 5 standard curve by two-site ELISA developed (closed diamonds), commercial available ELISA kit (closed boxes). (B) Comparison of rBlo t 5 (open circles) to rDer p 5 (closed triangles) as standard curve for the developed two-site ELISA. (C) Comparison of nBlo t 5 (*) and rBlo t 5 (open boxes).
Yi, F. C., Lee, B. W., Cheong, N., & Chua, K. Y. (2005). Quantification of Blo t 5 in mite and dust extracts by two‐site ELISA. Allergy, 60(1), 108-112.
Figure 2 The reactivity of mAb 4A7 to Blo t 5 isoforms was shown in this figure.
Two dimensional electrophoresis and immunoblotting of Blomia tropicalis extract. Fifty micrograms of mite extracts were focused on 13 cm, pH 4–7 linear IPG strips and separated on 15% polyarylamide gel. (A) Two-dimensional protein profile of mite extract. Immunoblots of mite extract incubate with mouse anti-Blo t 5 polyclonal serum (B), Blo t 5 specific monoclonal antibody (C), and naive mouse serum (D).
Yi, F. C., Lee, B. W., Cheong, N., & Chua, K. Y. (2005). Quantification of Blo t 5 in mite and dust extracts by two‐site ELISA. Allergy, 60(1), 108-112.
Specifications
- Immunogen
- B. tropicalis Mite allergen Blo t 5
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native B. tropicalis Blo t 5
- Species Reactivity
- B. tropicalis
- Clone
- 4A7
- Applications
- ELISA Assay-Dependent
WB 1:1000-1:5000
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The antibody 4A7 has been reported in applications of ELISA, WB. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
Target
- Alternative Names
- Blo t 5; BLOT5; Mite allergen Blo t 5
- UniProt ID
- O96870
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBZ-136 | Recombinant Mouse Anti-B. tropicalis Blo t 5 Antibody Fab Fragment (4A7) | ELISA | Fab |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBZ-136 | Recombinant Mouse Anti-B. tropicalis Blo t 5 Antibody scFv Fragment (4A7) | ELISA | scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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