Recombinant Human Anti-Betula Bet v1 Antibody (BV16) (CAT#: PABZ-014)
Recombinant Human Antibody (BV16) is capable of binding to Betula Bet v1, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-Betula Bet v1 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-Betula Bet v1 mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. BV16 inhibits the binding of human serum IgE to Bet v 1 by ∼40%. Moreover, the BV16 mAb shows cross-reactivity with the homologous major allergens from alder and hornbeam.
Specific Inquiry
Figure 1 kinetic parameters for the binding of mAb BV16 are shown below each model.
No binding to Mal d 1 could be detected, whereas very similar kinetic parameters characterize BV16 antibody binding to Bet v 1 and Mut-2782 were found.
Holm, J., Ferreras, M., Ipsen, H., Würtzen, P. A., Gajhede, M., Larsen, J. N., ... & Spangfort, M. D. (2011). Epitope grafting, re-creating a conformational Bet v 1 antibody epitope on the surface of the homologous apple allergen Mal d 1. Journal of Biological Chemistry, 286(20), 17569-17578.
IB
Figure 2 Immunoblotting of mAb BV16. Lane a, m.w. standards (kDa).
Lanes b-e, pollen extracts from birch (lane b), hazel (lane c), alder (lane d), and hornbeam (lane e). Lane f, Recombinant Bet v 1.
Mirza, O., Henriksen, A., Ipsen, H., Larsen, J. N., Wissenbach, M., Spangfort, M. D., & Gajhede, M. (2000). Dominant epitopes and allergic cross-reactivity: complex formation between a Fab fragment of a monoclonal murine IgG antibody and the major allergen from birch pollen Bet v 1. The Journal of Immunology, 165(1), 331-338.
Inhib
Figure 3 Serum IgE inhibition assay.
Inhibition of the binding of biotinylated Bet v 1 to a pool of birch pollen-allergic patients' serum IgE by Ab preparations. ♦, Rabbit polyclonal anti-crude birch pollen extract; ▪, rabbit polyclonal anti-Bet v 1 (X) murine monoclonal BV16; ▴, rabbit polyclonal anti-crude house dust mite extract. CL is confidence limits.
Mirza, O., Henriksen, A., Ipsen, H., Larsen, J. N., Wissenbach, M., Spangfort, M. D., & Gajhede, M. (2000). Dominant epitopes and allergic cross-reactivity: complex formation between a Fab fragment of a monoclonal murine IgG antibody and the major allergen from birch pollen Bet v 1. The Journal of Immunology, 165(1), 331-338.
Specifications
- Immunogen
- BetulaBet v I allergen
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native Betula Bet v1
- Species Reactivity
- Betula
- Clone
- BV16
- Applications
- Can be useful in applications such as: Enzyme-linked Immunosorbent Assay
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Target
- Alternative Names
- Bet v I allergen
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBZ-014 | Recombinant Human Anti-Betula Bet v1 Antibody scFv Fragment (BV16) | ELISA | scFv |
| HPAB-1515WJ-S(P) | Human Anti-Bet v I Recombinant Antibody; scFv Fragment (clone G17E7A1) | ELISA, WB, IP | Human scFv |
| HPAB-1516WJ-S(P) | Human Anti-Bet v I Recombinant Antibody; scFv Fragment (clone G27E9B1) | ELISA, WB, IP | Human scFv |
| HPAB-1517WJ-S(P) | Human Anti-Bet v I Recombinant Antibody; scFv Fragment (clone G5F2A4) | ELISA, WB, IP | Human scFv |
| HPAB-1518WJ-S(P) | Human Anti-Bet v I Recombinant Antibody; scFv Fragment (clone G11A9F3) | ELISA, WB, IP | Human scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1515WJ-F(E) | Human Anti-Bet v I Recombinant Antibody; Fab Fragment (clone G17E7A1) | ELISA, WB, IP | Human Fab |
| HPAB-1516WJ-F(E) | Human Anti-Bet v I Recombinant Antibody; Fab Fragment (clone G27E9B1) | ELISA, WB, IP | Human Fab |
| HPAB-1517WJ-F(E) | Human Anti-Bet v I Recombinant Antibody; Fab Fragment (clone G5F2A4) | ELISA, WB, IP | Human Fab |
| HPAB-1518WJ-F(E) | Human Anti-Bet v I Recombinant Antibody; Fab Fragment (clone G11A9F3) | ELISA, WB, IP | Human Fab |
| HPAB-1519WJ-F(E) | Human Anti-Bet v I Recombinant Antibody; Fab Fragment (clone G28C10C10) | ELISA, WB, IP | Human Fab |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1515WJ | Human Anti-Bet v I Recombinant Antibody (clone G17E7A1) | ELISA, WB, IP | Human IgG |
| HPAB-1516WJ | Human Anti-Bet v I Recombinant Antibody (clone G27E9B1) | ELISA, WB, IP | Human IgG |
| HPAB-1517WJ | Human Anti-Bet v I Recombinant Antibody (clone G5F2A4) | ELISA, WB, IP | Human IgG |
| HPAB-1518WJ | Human Anti-Bet v I Recombinant Antibody (clone G11A9F3) | ELISA, WB, IP | Human IgG |
| HPAB-1519WJ | Human Anti-Bet v I Recombinant Antibody (clone G28C10C10) | ELISA, WB, IP | Human IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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