Anti-Human CD22 Recombinant Antibody (TAB-176) (CAT#: TAB-176)
Recombinant monoclonal antibody to CD22. It is a humanized monoclonal antibody. Potential uses may be found in oncology and in treatment of inflammatory autoimmune disorders, such as lupus (SLE).
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WB
Figure 1 Anti-Human CD22 Recombinant Antibody (TAB-176) in WB
Western blot analysis of TAB-176 was performed with Recombinant Human CD22 Protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-176 and HRP Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
ELISA
Figure 2 Anti-Human CD22 Recombinant Antibody (TAB-176) in ELISA
ELISA analysis of TAB-176 was performed by coating with Recombinant Human CD22 Protein. Then blocked with BSA, and incubated with Anti-Human CD22 Antibody (TAB-176). The HRP-conjugated anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Immunogen
- Human tonsil lymphocytes.
- Host Species
- Mouse
- Derivation
- Humanized (from mouse)
- Type
- IgG1
- Specificity
- Tested positive against native human antigen.
- Species Reactivity
- Human
- Applications
- IF, IP, Neut, FuncS, ELISA, FC, ICC, Activ, WB
- Related Disease
- B cell lymphoma
Product Property
- Purity
- >95.0%, determined by analysis by RP-HPLC & analysis by SDS-PAGE.
- Storage
- Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Applications
- Application Notes
- The CD22 antibody has been reported in applications of IF, IP, Neut, FuncS, ELISA, FC, ICC, Activ, WB.
Target
- Alternative Names
- Epratuzumab;LymphoCide;205923-57-5;AMG 412;hLL2;DB04958CD22;CD22 molecule;CD22 antigen;B-cell receptor CD22;sialic acid binding Ig like lectin 2;SIGLEC 2;SIGLEC2;BL-CAM;T-cell surface antigen Leu-14;B-lymphocyte cell adhesion molecule;sialic acid binding
- Gene ID
- 933
- UniProt ID
- P20273
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-L048E | anti-CD22 immunotoxin RFB4 (scFv)-PE | Cytotoxicity assay, Functional assay | |
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-176, RRID: AB_3111849)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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