Anti-Human CD22 Recombinant Antibody (hLL2) (CAT#: TAB-0196CL)

The anti-CD22 Antibody-Drug Conjugates comprise a drug moiety, an anti-CD22 antibody and a hydrophilic self-immolative linker. The antibody in this ADC compound specifically target human cluster of differentiation-22, which belongs to the SIGLEC family of lections. Therefore the anti-CD22 ADC compound can be used in the treatment or diagnosis of hematological malignancy.

Host: Human

Applications: Inhib, Cyt, FC, WB


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Inhib

Figure 1 Evaluation of growth-inhibition and apoptosis in D1–1 and Ramos cells.

Cell viabilitydetermined by the MTS assay after 4-day incubation for the Dried-I format of epratuzumab(hLL2*) or labetuzumab (hMN-14*).

Chang, C. H., Wang, Y., Gupta, P., & Goldenberg, D. M. (2015, January). Extensive crosslinking of CD22 by epratuzumab triggers BCR signaling and caspase-dependent apoptosis in human lymphoma cells. In MAbs (Vol. 7, No. 1, pp. 199-211). Taylor & Francis.

Cyt

Figure 2 Cytotoxicity of epratuzumab in various formats to Daudi cells.

Epratuzumab pre-sented as the Dried-I (hLL2*) or Wet-III (hLL2 C GAH C anti-IgM) format (right panel), but not theWet-I (hLL2) or Wet-IIB (hLL2 C GAH) format (left panel), induced dose-dependent cytotoxicity inDaudi cells, as measured by the MTS assay.

Chang, C. H., Wang, Y., Gupta, P., & Goldenberg, D. M. (2015, January). Extensive crosslinking of CD22 by epratuzumab triggers BCR signaling and caspase-dependent apoptosis in human lymphoma cells. In MAbs (Vol. 7, No. 1, pp. 199-211). Taylor & Francis.

FC

Figure 3 Cytotoxicity of epratuzumab in various formats to Daudi cells.

The Dried-I format of epratuzumab (hLL2*)induced apoptosis comparable to the positive control (anti-IgM) as determined by the Annexin Vassay.

Chang, C. H., Wang, Y., Gupta, P., & Goldenberg, D. M. (2015, January). Extensive crosslinking of CD22 by epratuzumab triggers BCR signaling and caspase-dependent apoptosis in human lymphoma cells. In MAbs (Vol. 7, No. 1, pp. 199-211). Taylor & Francis.

WB

Figure 4 Phosphorylation of CD79a, CD79b, and CD22.

Western blot analyses of phosphorylated CD79a, CD79b,and CD22 in D1–1 cells treated for 2 h with various formats of soluble epratuzumab, including Wet-I(lane 4; hLL2, 7.5 mg/mL), Wet-IIA (lane 5; hLL2, 7.5 mg/mL; GAH, 10 mg/mL), and Wet-III (lane 7; hLL2, 7.5 mg/mL;GAH, 10 mg/mL; anti-IgM, 1 mg/mL). In lane 6, the amounts of GAH and anti-IgM were the same as those in lane 7, but the concentration of epratuzumab was too low (10 ng/mL) to induce a notable effect.

Chang, C. H., Wang, Y., Gupta, P., & Goldenberg, D. M. (2015, January). Extensive crosslinking of CD22 by epratuzumab triggers BCR signaling and caspase-dependent apoptosis in human lymphoma cells. In MAbs (Vol. 7, No. 1, pp. 199-211). Taylor & Francis.


Specifications

  • Host Species
  • Human
  • Specificity
  • Human
  • Clone
  • hLL2
  • Applications
  • Inhib, Cyt, FC, WB
  • Related Disease
  • Hematological malignancy

Applications

  • Application Notes
  • This antibody has been reported for use in Inhibition, Cytotoxicity, Flow Cytometry and Western Blot.

Target

  • Alternative Names
  • CD22; CD22 molecule; SIGLEC2; SIGLEC-2; B-cell receptor CD22; BL-CAM; CD22 antigen; T-cell surface antigen Leu-14; B-lymphocyte cell adhesion molecule; sialic acid binding Ig-like lectin 2; sialic acid-binding Ig-like lectin 2;

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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scFv Fragment Antibody

CAT Product Name Application Type
MOB-1248z-S(P) Recombinant Anti-Human CD22 Antibody scFv Fragment ELISA, WB, IF, FuncS scFv
HPAB-0249-FY-S(P) Recombinant Human Anti-CD22 Antibody scFv Fragment F Human scFv
HPAB-0034-YJ-S(P) Mouse Anti-CD22 Recombinant Antibody (clone HB22-23); scFv Fragment Block Mouse scFv
HPAB-0709-FY-F(E) Mouse Anti-CD22 Recombinant Antibody (clone AB008); scFv Fragment ELISA Mouse scFv
HPAB-0370-FY-S(P) Human Anti-CD22 Recombinant Antibody; scFv Fragment (HPAB-0370-FY-S(P)) ELISA Humanized scFv

Immunotoxin

CAT Product Name Application Type
AGTO-G077E Anti-CD22 immunotoxin RFB4 (IgG)-PE Cytotoxicity assay, Function study
AGTO-G077D Anti-CD22 immunotoxin RFB4 (IgG)-DT Cytotoxicity assay, Function study
AGTO-G077G Anti-CD22 immunotoxin RFB4 (IgG)-Gel Cytotoxicity assay, Function study
AGTO-G077R Anti-CD22 immunotoxin RFB4 (IgG)-RTA Cytotoxicity assay, Function study
AGTO-G077S Anti-CD22 immunotoxin RFB4 (IgG)-Sap Cytotoxicity assay, Function study

Mouse Antibody

CAT Product Name Application Type
TAB-1714CL Mouse Anti-CD22 Recombinant Antibody (TAB-1714CL) ELISA, WB Mouse IgG
TAB-1728CL Anti-Human CD22 Recombinant Antibody (RFB4) IF, FC
TAB-1714CL-F(E) Mouse Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-1714CL-F(E)) ELISA, WB Mouse Fab
TAB-1723CL-F(E) Mouse Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-1723CL-F(E)) Inhib Mouse Fab
TAB-1728CL-F(E) Anti-Human CD22 Recombinant Antibody Fab Fragment (RFB4) IF, FC

Chimeric Antibody

CAT Product Name Application Type
TAB-1724CL Human Anti-CD22 Recombinant Antibody (TAB-1724CL) Inhib Chimeric (mouse/human) IgG1, κ
TAB-1724CL-S(P) Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (TAB-1724CL-S(P)) Inhib Mouse scFv
TAB-001LC Human Anti-CD22 Recombinant Antibody (TAB-001LC) ELISA Chimeric antibody (mouse/human)
TAB-001LC-S(P) Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (TAB-001LC-S(P)) ELISA Mouse scFv
TAB-001LC-F(E) Human Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-001LC-F(E)) ELISA Chimeric antibody (mouse/human)

Humanized Antibody

CAT Product Name Application Type
TAB-1725CL Human Anti-CD22 Recombinant Antibody (TAB-1725CL) Block, Cyt, ELISA, FuncS Humanized IgG
TAB-1729CL Human Anti-CD22 Recombinant Antibody (TAB-1729CL) IF, FC Humanized IgG
TAB-1725CL-F(E) Human Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-1725CL-F(E)) Block, Cyt, ELISA, FuncS Humanized Fab
TAB-1729CL-F(E) Human Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-1729CL-F(E)) IF, FC Humanized Fab
TAB-002LC-S(P) Anti-Human CD22 Recombinant Antibody scFv Fragment (VM1000) ELISA, SPR Humanized antibody

Human Antibody

CAT Product Name Application Type
TAB-1721CL-S(P) Anti-Human CD22 Recombinant Antibody scFv Fragment (CD22.1) ELISA, FC, FuncS Human antibody
TAB-1722CL-S(P) Anti-Human CD22 Recombinant Antibody scFv Fragment (CD22.2) ELISA, FC, FuncS Human antibody
TAB-0196CL-F(E) Anti-Human CD22 Recombinant Antibody Fab Fragment (hLL2) ELISA, In vivo
TAB-0197CL-F(E) Anti-Human CD22 Recombinant Antibody Fab Fragment (hLL2-Cys) ELISA, In vivo
TAB-1721CL-F(E) Anti-Human CD22 Recombinant Antibody Fab Fragment (CD22.1) ELISA, FC, FuncS Human antibody

Fab Glycosylation

CAT Product Name Application Type
Gly-001LC Recombinant Anti-Human CD22 Antibody (Fab glycosylation) ELISA, FC Mouse antibody

Fc Glycosylation

CAT Product Name Application Type
Gly-111LC Recombinant Anti-Human CD22 Antibody (Fc glycosylation) ELISA Chimeric antibody (mouse/human)

Blocking Antibody

CAT Product Name Application Type
NEUT-315CQ Rat Anti-CD22 Recombinant Antibody (clone 2D6) Block Rat IgG1, κ

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0545 Hi-Affi™ Rabbit Anti-CD22 Recombinant Antibody (clone DS545AB) IP, WB Rabbit IgG

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-0194-LSX-F(E) Mouse Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0194-LSX-F(E)) ELISA Mouse Fab
HPAB-0195-LSX-F(E) Human Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0195-LSX-F(E)) ELISA Human Fab
HPAB-0196-LSX-F(E) Mouse Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0196-LSX-F(E)) ELISA Mouse Fab
HPAB-0197-LSX-F(E) Human Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0197-LSX-F(E)) ELISA Human Fab
HPAB-0198-LSX-F(E) Mouse Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0198-LSX-F(E)) ELISA, FC Mouse Fab

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-176 Afuco™ Anti-CD22 ADCC Recombinant Antibody (Epratuzumab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody
AFC-TAB-198 Afuco™ Anti-CD22 ADCC Recombinant Antibody (Inotuzumab), ADCC Enhanced ELISA, FC, IP, FuncS, IF, Neut ADCC enhanced antibody

Recombinant Antibody

CAT Product Name Application Type
FAMAB-1612CQ Mouse Anti-CD22 Recombinant Antibody (clone OX97) WB, FC Mouse IgG2a
ZG-0989F Mouse Anti-CD22 Recombinant Antibody (clone 3H4) FC, IHC-Fr, ICC, IHC-Fr Mouse IgG1
VS3-WK3 Rabbit Anti-CD22 Recombinant Antibody (clone DM12) ELISA, FC, WB, IF Rabbit IgG
VS3-WK4 Rabbit Anti-CD22 Recombinant Antibody (clone DM13) ELISA, FC, WB, IP, IF Rabbit IgG
VS3-WK5 Rabbit Anti-CD22 Recombinant Antibody (clone DM14) ELISA, FC, IP, IF Rabbit IgG

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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