The anti-CD22 Antibody-Drug Conjugates comprise a drug moiety, an anti-CD22 antibody and a hydrophilic self-immolative linker. The antibody in this ADC compound specifically target human cluster of differentiation-22, which belongs to the SIGLEC family of lections. Therefore the anti-CD22 ADC compound can be used in the treatment or diagnosis of hematological malignancy.
Host: Human
Applications: Inhib, Cyt, FC, WB
Figure 1 Evaluation of growth-inhibition and apoptosis in D1–1 and Ramos cells.
Cell viabilitydetermined by the MTS assay after 4-day incubation for the Dried-I format of epratuzumab(hLL2*) or labetuzumab (hMN-14*).
Chang, C. H., Wang, Y., Gupta, P., & Goldenberg, D. M. (2015, January). Extensive crosslinking of CD22 by epratuzumab triggers BCR signaling and caspase-dependent apoptosis in human lymphoma cells. In MAbs (Vol. 7, No. 1, pp. 199-211). Taylor & Francis.
Figure 2 Cytotoxicity of epratuzumab in various formats to Daudi cells.
Epratuzumab pre-sented as the Dried-I (hLL2*) or Wet-III (hLL2 C GAH C anti-IgM) format (right panel), but not theWet-I (hLL2) or Wet-IIB (hLL2 C GAH) format (left panel), induced dose-dependent cytotoxicity inDaudi cells, as measured by the MTS assay.
Chang, C. H., Wang, Y., Gupta, P., & Goldenberg, D. M. (2015, January). Extensive crosslinking of CD22 by epratuzumab triggers BCR signaling and caspase-dependent apoptosis in human lymphoma cells. In MAbs (Vol. 7, No. 1, pp. 199-211). Taylor & Francis.
Figure 3 Cytotoxicity of epratuzumab in various formats to Daudi cells.
The Dried-I format of epratuzumab (hLL2*)induced apoptosis comparable to the positive control (anti-IgM) as determined by the Annexin Vassay.
Chang, C. H., Wang, Y., Gupta, P., & Goldenberg, D. M. (2015, January). Extensive crosslinking of CD22 by epratuzumab triggers BCR signaling and caspase-dependent apoptosis in human lymphoma cells. In MAbs (Vol. 7, No. 1, pp. 199-211). Taylor & Francis.
Figure 4 Phosphorylation of CD79a, CD79b, and CD22.
Western blot analyses of phosphorylated CD79a, CD79b,and CD22 in D1–1 cells treated for 2 h with various formats of soluble epratuzumab, including Wet-I(lane 4; hLL2, 7.5 mg/mL), Wet-IIA (lane 5; hLL2, 7.5 mg/mL; GAH, 10 mg/mL), and Wet-III (lane 7; hLL2, 7.5 mg/mL;GAH, 10 mg/mL; anti-IgM, 1 mg/mL). In lane 6, the amounts of GAH and anti-IgM were the same as those in lane 7, but the concentration of epratuzumab was too low (10 ng/mL) to induce a notable effect.
Chang, C. H., Wang, Y., Gupta, P., & Goldenberg, D. M. (2015, January). Extensive crosslinking of CD22 by epratuzumab triggers BCR signaling and caspase-dependent apoptosis in human lymphoma cells. In MAbs (Vol. 7, No. 1, pp. 199-211). Taylor & Francis.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
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CAT | Product Name | Application | Type |
MOB-1248z-S(P) | Recombinant Anti-Human CD22 Antibody scFv Fragment | ELISA, WB, IF, FuncS | scFv |
HPAB-0249-FY-S(P) | Recombinant Human Anti-CD22 Antibody scFv Fragment | F | Human scFv |
HPAB-0034-YJ-S(P) | Mouse Anti-CD22 Recombinant Antibody (clone HB22-23); scFv Fragment | Block | Mouse scFv |
HPAB-0709-FY-F(E) | Mouse Anti-CD22 Recombinant Antibody (clone AB008); scFv Fragment | ELISA | Mouse scFv |
HPAB-0370-FY-S(P) | Human Anti-CD22 Recombinant Antibody; scFv Fragment (HPAB-0370-FY-S(P)) | ELISA | Humanized scFv |
CAT | Product Name | Application | Type |
AGTO-G077E | Anti-CD22 immunotoxin RFB4 (IgG)-PE | Cytotoxicity assay, Function study | |
AGTO-G077D | Anti-CD22 immunotoxin RFB4 (IgG)-DT | Cytotoxicity assay, Function study | |
AGTO-G077G | Anti-CD22 immunotoxin RFB4 (IgG)-Gel | Cytotoxicity assay, Function study | |
AGTO-G077R | Anti-CD22 immunotoxin RFB4 (IgG)-RTA | Cytotoxicity assay, Function study | |
AGTO-G077S | Anti-CD22 immunotoxin RFB4 (IgG)-Sap | Cytotoxicity assay, Function study |
CAT | Product Name | Application | Type |
TAB-1714CL | Mouse Anti-CD22 Recombinant Antibody (TAB-1714CL) | ELISA, WB | Mouse IgG |
TAB-1728CL | Anti-Human CD22 Recombinant Antibody (RFB4) | IF, FC | |
TAB-1714CL-F(E) | Mouse Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-1714CL-F(E)) | ELISA, WB | Mouse Fab |
TAB-1723CL-F(E) | Mouse Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-1723CL-F(E)) | Inhib | Mouse Fab |
TAB-1728CL-F(E) | Anti-Human CD22 Recombinant Antibody Fab Fragment (RFB4) | IF, FC |
CAT | Product Name | Application | Type |
TAB-1724CL | Human Anti-CD22 Recombinant Antibody (TAB-1724CL) | Inhib | Chimeric (mouse/human) IgG1, κ |
TAB-1724CL-S(P) | Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (TAB-1724CL-S(P)) | Inhib | Mouse scFv |
TAB-001LC | Human Anti-CD22 Recombinant Antibody (TAB-001LC) | ELISA | Chimeric antibody (mouse/human) |
TAB-001LC-S(P) | Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (TAB-001LC-S(P)) | ELISA | Mouse scFv |
TAB-001LC-F(E) | Human Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-001LC-F(E)) | ELISA | Chimeric antibody (mouse/human) |
CAT | Product Name | Application | Type |
TAB-1725CL | Human Anti-CD22 Recombinant Antibody (TAB-1725CL) | Block, Cyt, ELISA, FuncS | Humanized IgG |
TAB-1729CL | Human Anti-CD22 Recombinant Antibody (TAB-1729CL) | IF, FC | Humanized IgG |
TAB-1725CL-F(E) | Human Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-1725CL-F(E)) | Block, Cyt, ELISA, FuncS | Humanized Fab |
TAB-1729CL-F(E) | Human Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-1729CL-F(E)) | IF, FC | Humanized Fab |
TAB-002LC-S(P) | Anti-Human CD22 Recombinant Antibody scFv Fragment (VM1000) | ELISA, SPR | Humanized antibody |
CAT | Product Name | Application | Type |
TAB-1721CL-S(P) | Anti-Human CD22 Recombinant Antibody scFv Fragment (CD22.1) | ELISA, FC, FuncS | Human antibody |
TAB-1722CL-S(P) | Anti-Human CD22 Recombinant Antibody scFv Fragment (CD22.2) | ELISA, FC, FuncS | Human antibody |
TAB-0196CL-F(E) | Anti-Human CD22 Recombinant Antibody Fab Fragment (hLL2) | ELISA, In vivo | |
TAB-0197CL-F(E) | Anti-Human CD22 Recombinant Antibody Fab Fragment (hLL2-Cys) | ELISA, In vivo | |
TAB-1721CL-F(E) | Anti-Human CD22 Recombinant Antibody Fab Fragment (CD22.1) | ELISA, FC, FuncS | Human antibody |
CAT | Product Name | Application | Type |
Gly-001LC | Recombinant Anti-Human CD22 Antibody (Fab glycosylation) | ELISA, FC | Mouse antibody |
CAT | Product Name | Application | Type |
Gly-111LC | Recombinant Anti-Human CD22 Antibody (Fc glycosylation) | ELISA | Chimeric antibody (mouse/human) |
CAT | Product Name | Application | Type |
NEUT-315CQ | Rat Anti-CD22 Recombinant Antibody (clone 2D6) | Block | Rat IgG1, κ |
CAT | Product Name | Application | Type |
MOR-0545 | Hi-Affi™ Rabbit Anti-CD22 Recombinant Antibody (clone DS545AB) | IP, WB | Rabbit IgG |
CAT | Product Name | Application | Type |
HPAB-0194-LSX-F(E) | Mouse Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0194-LSX-F(E)) | ELISA | Mouse Fab |
HPAB-0195-LSX-F(E) | Human Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0195-LSX-F(E)) | ELISA | Human Fab |
HPAB-0196-LSX-F(E) | Mouse Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0196-LSX-F(E)) | ELISA | Mouse Fab |
HPAB-0197-LSX-F(E) | Human Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0197-LSX-F(E)) | ELISA | Human Fab |
HPAB-0198-LSX-F(E) | Mouse Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0198-LSX-F(E)) | ELISA, FC | Mouse Fab |
CAT | Product Name | Application | Type |
AFC-TAB-176 | Afuco™ Anti-CD22 ADCC Recombinant Antibody (Epratuzumab), ADCC Enhanced | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
AFC-TAB-198 | Afuco™ Anti-CD22 ADCC Recombinant Antibody (Inotuzumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
CAT | Product Name | Application | Type |
FAMAB-1612CQ | Mouse Anti-CD22 Recombinant Antibody (clone OX97) | WB, FC | Mouse IgG2a |
ZG-0989F | Mouse Anti-CD22 Recombinant Antibody (clone 3H4) | FC, IHC-Fr, ICC, IHC-Fr | Mouse IgG1 |
VS3-WK3 | Rabbit Anti-CD22 Recombinant Antibody (clone DM12) | ELISA, FC, WB, IF | Rabbit IgG |
VS3-WK4 | Rabbit Anti-CD22 Recombinant Antibody (clone DM13) | ELISA, FC, WB, IP, IF | Rabbit IgG |
VS3-WK5 | Rabbit Anti-CD22 Recombinant Antibody (clone DM14) | ELISA, FC, IP, IF | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.