Anti-Human CD22 Recombinant Antibody (hLL2) (CAT#: TAB-0196CL)

The anti-CD22 Antibody-Drug Conjugates comprise a drug moiety, an anti-CD22 antibody and a hydrophilic self-immolative linker. The antibody in this ADC compound specifically target human cluster of differentiation-22, which belongs to the SIGLEC family of lections. Therefore the anti-CD22 ADC compound can be used in the treatment or diagnosis of hematological malignancy.

Host: Human

Applications: Inhib, Cyt, FC, WB


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Inhib

Figure 1 Evaluation of growth-inhibition and apoptosis in D1–1 and Ramos cells.

Cell viabilitydetermined by the MTS assay after 4-day incubation for the Dried-I format of epratuzumab(hLL2*) or labetuzumab (hMN-14*).

Chang, C. H., Wang, Y., Gupta, P., & Goldenberg, D. M. (2015, January). Extensive crosslinking of CD22 by epratuzumab triggers BCR signaling and caspase-dependent apoptosis in human lymphoma cells. In MAbs (Vol. 7, No. 1, pp. 199-211). Taylor & Francis.

Cyt

Figure 2 Cytotoxicity of epratuzumab in various formats to Daudi cells.

Epratuzumab pre-sented as the Dried-I (hLL2*) or Wet-III (hLL2 C GAH C anti-IgM) format (right panel), but not theWet-I (hLL2) or Wet-IIB (hLL2 C GAH) format (left panel), induced dose-dependent cytotoxicity inDaudi cells, as measured by the MTS assay.

Chang, C. H., Wang, Y., Gupta, P., & Goldenberg, D. M. (2015, January). Extensive crosslinking of CD22 by epratuzumab triggers BCR signaling and caspase-dependent apoptosis in human lymphoma cells. In MAbs (Vol. 7, No. 1, pp. 199-211). Taylor & Francis.

FC

Figure 3 Cytotoxicity of epratuzumab in various formats to Daudi cells.

The Dried-I format of epratuzumab (hLL2*)induced apoptosis comparable to the positive control (anti-IgM) as determined by the Annexin Vassay.

Chang, C. H., Wang, Y., Gupta, P., & Goldenberg, D. M. (2015, January). Extensive crosslinking of CD22 by epratuzumab triggers BCR signaling and caspase-dependent apoptosis in human lymphoma cells. In MAbs (Vol. 7, No. 1, pp. 199-211). Taylor & Francis.

WB

Figure 4 Phosphorylation of CD79a, CD79b, and CD22.

Western blot analyses of phosphorylated CD79a, CD79b,and CD22 in D1–1 cells treated for 2 h with various formats of soluble epratuzumab, including Wet-I(lane 4; hLL2, 7.5 mg/mL), Wet-IIA (lane 5; hLL2, 7.5 mg/mL; GAH, 10 mg/mL), and Wet-III (lane 7; hLL2, 7.5 mg/mL;GAH, 10 mg/mL; anti-IgM, 1 mg/mL). In lane 6, the amounts of GAH and anti-IgM were the same as those in lane 7, but the concentration of epratuzumab was too low (10 ng/mL) to induce a notable effect.

Chang, C. H., Wang, Y., Gupta, P., & Goldenberg, D. M. (2015, January). Extensive crosslinking of CD22 by epratuzumab triggers BCR signaling and caspase-dependent apoptosis in human lymphoma cells. In MAbs (Vol. 7, No. 1, pp. 199-211). Taylor & Francis.


Specifications

  • Host Species
  • Human
  • Specificity
  • Human
  • Clone
  • hLL2
  • Applications
  • Inhib, Cyt, FC, WB
  • Related Disease
  • Hematological malignancy

Applications

  • Application Notes
  • This antibody has been reported for use in Inhibition, Cytotoxicity, Flow Cytometry and Western Blot.

Target

  • Alternative Names
  • CD22; CD22 molecule; SIGLEC2; SIGLEC-2; B-cell receptor CD22; BL-CAM; CD22 antigen; T-cell surface antigen Leu-14; B-lymphocyte cell adhesion molecule; sialic acid binding Ig-like lectin 2; sialic acid-binding Ig-like lectin 2;

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Recombinant Antibody

CAT Product Name Application Type
MOB-1248z Recombinant Anti-Human CD22 Antibody WB, IHC, FuncS IgG
MOB-0349MZ Mouse Anti-CD22 Recombinant Antibody (clone 5LB129) ELISA Mouse IgG1
FAMAB-0046YC Mouse Anti-CD22 Recombinant Antibody (clone m5/44) ELISA, Cyt Mouse IgG1
FAMAB-0047YC Human Anti-CD22 Recombinant Antibody (clone g5/44) ELISA, Cyt Humanized IgG4
FAMAB-0048YC Human Anti-CD22 Recombinant Antibody (clone ch5/44) ELISA Chimeric (mouse/human) IgG

Humanized Antibody

CAT Product Name Application Type
TAB-899 Humanized Anti-CD22 Recombinant Antibody (clone Pinatuzumab) FC, IP, ELISA, Neut, FuncS, IF, ICC Humanized (from mouse) IgG1, κ
TAB-1725CL-F(E) Human Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-1725CL-F(E)) Block, Cyt, ELISA, FuncS Humanized Fab
TAB-1729CL-F(E) Human Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-1729CL-F(E)) IF, FC Humanized Fab
TAB-002LC Anti-Human CD22 Recombinant Antibody (VM1000) FC, ELISA, SPR, FuncS Humanized antibody
TAB-002LC-F(E) Anti-Human CD22 Recombinant Antibody Fab Fragment (VM1000) ELISA, SPR Humanized antibody

Immunotoxin

CAT Product Name Application Type
AGTO-L048E anti-CD22 immunotoxin RFB4 (scFv)-PE Cytotoxicity assay, Functional assay
AGTO-L048D anti-CD22 immunotoxin RFB4 (scFv)-DT Cytotoxicity assay, Functional assay
AGTO-L048G anti-CD22 immunotoxin RFB4 (scFv)-Gel Cytotoxicity assay, Functional assay
AGTO-L048R anti-CD22 immunotoxin RFB4 (scFv)-RTA Cytotoxicity assay, Functional assay
AGTO-L048S anti-CD22 immunotoxin RFB4 (scFv)-Sap Cytotoxicity assay, Functional assay

Mouse Antibody

CAT Product Name Application Type
TAB-1714CL Mouse Anti-CD22 Recombinant Antibody (TAB-1714CL) ELISA, WB Mouse IgG
TAB-1728CL Anti-Human CD22 Recombinant Antibody (RFB4) IF, FC
TAB-1714CL-S(P) Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (TAB-1714CL-S(P)) ELISA, WB Mouse scFv
TAB-1723CL-S(P) Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (TAB-1723CL-S(P)) Inhib Mouse scFv
TAB-1728CL-S(P) Anti-Human CD22 Recombinant Antibody scFv Fragment (RFB4) IF, FC

Human Antibody

CAT Product Name Application Type
TAB-1715CL Anti-Human CD22 Recombinant Antibody (12C5) Cyt, FuncS Fully human antibody
TAB-0196CL-S(P) Anti-Human CD22 Recombinant Antibody scFv Fragment (hLL2) ELISA, In vivo
TAB-0197CL-S(P) Anti-Human CD22 Recombinant Antibody scFv Fragment (hLL2-Cys) ELISA, In vivo
TAB-1721CL-S(P) Anti-Human CD22 Recombinant Antibody scFv Fragment (CD22.1) ELISA, FC, FuncS Human antibody
TAB-1722CL-S(P) Anti-Human CD22 Recombinant Antibody scFv Fragment (CD22.2) ELISA, FC, FuncS Human antibody

Chimeric Antibody

CAT Product Name Application Type
TAB-1724CL Human Anti-CD22 Recombinant Antibody (TAB-1724CL) Inhib Chimeric (mouse/human) IgG1, κ
TAB-1724CL-F(E) Human Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-1724CL-F(E)) Inhib Chimeric (mouse/human) Fab
TAB-001LC Human Anti-CD22 Recombinant Antibody (TAB-001LC) ELISA Chimeric antibody (mouse/human)
TAB-001LC-S(P) Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (TAB-001LC-S(P)) ELISA Mouse scFv
TAB-001LC-F(E) Human Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-001LC-F(E)) ELISA Chimeric antibody (mouse/human)

Fab Glycosylation

CAT Product Name Application Type
Gly-001LC Recombinant Anti-Human CD22 Antibody (Fab glycosylation) ELISA, FC Mouse antibody

Fc Glycosylation

CAT Product Name Application Type
Gly-111LC Recombinant Anti-Human CD22 Antibody (Fc glycosylation) ELISA Chimeric antibody (mouse/human)

Blocking Antibody

CAT Product Name Application Type
NEUT-315CQ Rat Anti-CD22 Recombinant Antibody (clone 2D6) Block Rat IgG1, κ

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0545 Hi-Affi™ Rabbit Anti-CD22 Recombinant Antibody (clone DS545AB) IP, WB Rabbit IgG

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-0194-LSX-F(E) Mouse Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0194-LSX-F(E)) ELISA Mouse Fab
HPAB-0195-LSX-F(E) Human Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0195-LSX-F(E)) ELISA Human Fab
HPAB-0196-LSX-F(E) Mouse Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0196-LSX-F(E)) ELISA Mouse Fab
HPAB-0197-LSX-F(E) Human Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0197-LSX-F(E)) ELISA Human Fab
HPAB-0198-LSX-F(E) Mouse Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0198-LSX-F(E)) ELISA, FC Mouse Fab

scFv Fragment Antibody

CAT Product Name Application Type
HPAB-0249-FY-S(P) Recombinant Human Anti-CD22 Antibody scFv Fragment F Human scFv
HPAB-1899LY-S(P) Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (HPAB-1899LY-S(P)) ELISA, Inhib Mouse scFv
HPAB-1900LY-S(P) Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (HPAB-1900LY-S(P)) ELISA, Inhib Mouse scFv
HPAB-1901LY-S(P) Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (HPAB-1901LY-S(P)) ELISA, Inhib Mouse scFv
HPAB-1902LY-S(P) Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (HPAB-1902LY-S(P)) ELISA, Inhib Mouse scFv

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-176 Afuco™ Anti-CD22 ADCC Recombinant Antibody (Epratuzumab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody
AFC-TAB-198 Afuco™ Anti-CD22 ADCC Recombinant Antibody (Inotuzumab), ADCC Enhanced ELISA, FC, IP, FuncS, IF, Neut ADCC enhanced antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
WBWestern Blot

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