Anti-Human CD22 Recombinant Antibody (hLL2) (CAT#: TAB-0196CL)

The anti-CD22 Antibody-Drug Conjugates comprise a drug moiety, an anti-CD22 antibody and a hydrophilic self-immolative linker. The antibody in this ADC compound specifically target human cluster of differentiation-22, which belongs to the SIGLEC family of lections. Therefore the anti-CD22 ADC compound can be used in the treatment or diagnosis of hematological malignancy.


Specific Inquiry
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  • Endotoxin:
  • Purity:
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  • Published Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Inhib

Figure 1 Evaluation of growth-inhibition and apoptosis in D1–1 and Ramos cells.

Figure 1 Evaluation of growth-inhibition and apoptosis in D1–1 and Ramos cells.

Cell viabilitydetermined by the MTS assay after 4-day incubation for the Dried-I format of epratuzumab(hLL2*) or labetuzumab (hMN-14*).

Chang, C. H., Wang, Y., Gupta, P., & Goldenberg, D. M. (2015, January). Extensive crosslinking of CD22 by epratuzumab triggers BCR signaling and caspase-dependent apoptosis in human lymphoma cells. In MAbs (Vol. 7, No. 1, pp. 199-211). Taylor & Francis.

Cyt

Figure 2 Cytotoxicity of epratuzumab in various formats to Daudi cells.

Figure 2 Cytotoxicity of epratuzumab in various formats to Daudi cells.

Epratuzumab pre-sented as the Dried-I (hLL2*) or Wet-III (hLL2 C GAH C anti-IgM) format (right panel), but not theWet-I (hLL2) or Wet-IIB (hLL2 C GAH) format (left panel), induced dose-dependent cytotoxicity inDaudi cells, as measured by the MTS assay.

Chang, C. H., Wang, Y., Gupta, P., & Goldenberg, D. M. (2015, January). Extensive crosslinking of CD22 by epratuzumab triggers BCR signaling and caspase-dependent apoptosis in human lymphoma cells. In MAbs (Vol. 7, No. 1, pp. 199-211). Taylor & Francis.

FC

Figure 3 Cytotoxicity of epratuzumab in various formats to Daudi cells.

Figure 3 Cytotoxicity of epratuzumab in various formats to Daudi cells.

The Dried-I format of epratuzumab (hLL2*)induced apoptosis comparable to the positive control (anti-IgM) as determined by the Annexin Vassay.

Chang, C. H., Wang, Y., Gupta, P., & Goldenberg, D. M. (2015, January). Extensive crosslinking of CD22 by epratuzumab triggers BCR signaling and caspase-dependent apoptosis in human lymphoma cells. In MAbs (Vol. 7, No. 1, pp. 199-211). Taylor & Francis.

WB

Figure 4 Phosphorylation of CD79a, CD79b, and CD22.

Figure 4 Phosphorylation of CD79a, CD79b, and CD22.

Western blot analyses of phosphorylated CD79a, CD79b,and CD22 in D1–1 cells treated for 2 h with various formats of soluble epratuzumab, including Wet-I(lane 4; hLL2, 7.5 mg/mL), Wet-IIA (lane 5; hLL2, 7.5 mg/mL; GAH, 10 mg/mL), and Wet-III (lane 7; hLL2, 7.5 mg/mL;GAH, 10 mg/mL; anti-IgM, 1 mg/mL). In lane 6, the amounts of GAH and anti-IgM were the same as those in lane 7, but the concentration of epratuzumab was too low (10 ng/mL) to induce a notable effect.

Chang, C. H., Wang, Y., Gupta, P., & Goldenberg, D. M. (2015, January). Extensive crosslinking of CD22 by epratuzumab triggers BCR signaling and caspase-dependent apoptosis in human lymphoma cells. In MAbs (Vol. 7, No. 1, pp. 199-211). Taylor & Francis.


Specifications

  • Host Species
  • Human
  • Specificity
  • Human
  • Clone
  • hLL2
  • Applications
  • Inhib, Cyt, FC, WB
  • Related Disease
  • Hematological malignancy

Applications

  • Application Notes
  • This antibody has been reported for use in Inhibition, Cytotoxicity, Flow Cytometry and Western Blot.

Target

  • Alternative Names
  • CD22; CD22 molecule; SIGLEC2; SIGLEC-2; B-cell receptor CD22; BL-CAM; CD22 antigen; T-cell surface antigen Leu-14; B-lymphocyte cell adhesion molecule; sialic acid binding Ig-like lectin 2; sialic acid-binding Ig-like lectin 2;

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Immunotoxin

CAT Product Name Application Type
AGTO-G077E Anti-CD22 immunotoxin RFB4 (IgG)-PE Cytotoxicity assay, Function study
AGTO-G077D Anti-CD22 immunotoxin RFB4 (IgG)-DT Cytotoxicity assay, Function study
AGTO-G077G Anti-CD22 immunotoxin RFB4 (IgG)-Gel Cytotoxicity assay, Function study
AGTO-G077R Anti-CD22 immunotoxin RFB4 (IgG)-RTA Cytotoxicity assay, Function study
AGTO-L048E anti-CD22 immunotoxin RFB4 (scFv)-PE Cytotoxicity assay, Functional assay

Fab Glycosylation

CAT Product Name Application Type
Gly-001LC Recombinant Anti-Human CD22 Antibody (Fab glycosylation) ELISA, FC Mouse antibody

Fc Glycosylation

CAT Product Name Application Type
Gly-111LC Recombinant Anti-Human CD22 Antibody (Fc glycosylation) ELISA Chimeric antibody (mouse/human)

Blocking Antibody

CAT Product Name Application Type
NEUT-315CQ Rat Anti-CD22 Recombinant Antibody (clone 2D6) Block Rat IgG1, κ

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0545 Hi-Affi™ Rabbit Anti-CD22 Recombinant Antibody (clone DS545AB) IP, WB Rabbit IgG

Recombinant Antibody

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-176 Afuco™ Anti-CD22 ADCC Recombinant Antibody (Epratuzumab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody
AFC-TAB-198 Afuco™ Anti-CD22 ADCC Recombinant Antibody (Inotuzumab), ADCC Enhanced ELISA, FC, IP, FuncS, IF, Neut ADCC enhanced antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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