Humanized Anti-CD22 Recombinant Antibody (clone Inotuzumab) (CAT#: TAB-198)
This product is a humanized (from mouse) IgG4, κ antibody that can recognize human CD22.
Specific Inquiry
ELISA
Figure 1 Anti-CD22 Antibody (TAB-198) in ELISA
ELISA analysis of TAB-198 was performed by coating with CD22 Protein, Human, Recombinant (His Tag).
The secondary antibody: HRP-Anti-Human IgG (H+L)
WB
Figure 2 Anti-CD22 Antibody (TAB-198) in WB
Western blot analysis of TAB-198 was performed with CD22 Protein, Human, Recombinant (His Tag).
TAB-198 incubation concentration: 2ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Lane 1: Reducing antigen (0.6μg)
DB
Figure 3 Anti-CD22 Antibody (TAB-198) in DB
Dot blot analysis of TAB-198 was performed with CD22 Protein, Human, Recombinant (His Tag).
TAB-198 incubation concentration: 2ng/μL.
NC: Negative control PC: Positive control
FC
Figure 4 Anti-CD22 Antibody (TAB-198) in Flow Cytometry
Flow cytometry analysis of Daudi cells with anti-CD22 antibody (Cat#TAB-198, Blue line) or isotype control antibody (Yellow line). A Goat Anti-human IgG (FITC) was used as the secondary antibody.
Specifications
- Immunogen
- Human tonsil lymphocytes
- Host Species
- Human
- Derivation
- Humanized (from mouse)
- Type
- Humanized (from mouse) IgG4, κ
- Specificity
- Human CD22
- Species Reactivity
- Human
- Clone
- Inotuzumab
- Applications
- ELISA, FC, IP, FuncS, IF, Neut, ICC
Product Property
- Purification
- Protein A/G Purified
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Flow Cytometry, Immunoprecipitation, Functional Assay, Immunofluorescence, Neutralization, Immunocytochemistry.
Target
- Alternative Names
- SIGLEC2; SIGLEC-2; B-cell receptor CD22; B-lymphocyte cell adhesion molecule; BL-CAM; CD22 antigen; T-cell surface antigen Leu-14; sialic acid-binding Ig-like lectin 2
- Gene ID
- 933
- UniProt ID
- P20273
Related Resources
Please refer to Inotuzumab Ozogamicin Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Inotuzumab Ozogamicin.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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|---|---|---|---|
| MOB-1248z-F(E) | Recombinant Anti-Human CD22 Antibody Fab Fragment | IP, RIA, FuncS | Fab |
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| HPAB-0709-FY-S(P) | Mouse Anti-CD22 Recombinant Antibody (clone AB008); Fab Fragment | ELISA | Mouse Fab |
Customer Reviews and Q&As
Customer Review
Q&As
Good for Immunofluorescence
We conjugated Inotuzumab with PE and used it in immunofluorescence. It provided clear and specific staining of B cells. Highly recommend.
Scalability for Large Studies
Able to scale up usage for large cohort studies without compromising on performance. Supports extensive research applications.
Minimal Cross-reactivity
We tested Inotuzumab for cross-reactivity, and it showed minimal non-specific binding. Great for our human-specific studies.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-198, RRID: AB_3111867)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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