Humanized Anti-CD22 Recombinant Antibody (clone Inotuzumab) (CAT#: TAB-198)
This product is a humanized (from mouse) IgG4, κ antibody that can recognize human CD22.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
ELISA
Figure 1 Anti-CD22 Antibody (TAB-198) in ELISA
ELISA analysis of TAB-198 was performed by coating with CD22 Protein, Human, Recombinant (His Tag).
The secondary antibody: HRP-Anti-Human IgG (H+L)
WB
Figure 2 Anti-CD22 Antibody (TAB-198) in WB
Western blot analysis of TAB-198 was performed with CD22 Protein, Human, Recombinant (His Tag).
TAB-198 incubation concentration: 2ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Lane 1: Reducing antigen (0.6μg)
DB
Figure 3 Anti-CD22 Antibody (TAB-198) in DB
Dot blot analysis of TAB-198 was performed with CD22 Protein, Human, Recombinant (His Tag).
TAB-198 incubation concentration: 2ng/μL.
NC: Negative control PC: Positive control
FC
Figure 4 Anti-CD22 Antibody (TAB-198) in Flow Cytometry
Flow cytometry analysis of Daudi cells with anti-CD22 antibody (Cat#TAB-198, Blue line) or isotype control antibody (Yellow line). A Goat Anti-human IgG (FITC) was used as the secondary antibody.
Specifications
- Immunogen
- Human tonsil lymphocytes
- Host Species
- Human
- Derivation
- Humanized (from mouse)
- Type
- Humanized (from mouse) IgG4, κ
- Specificity
- Human CD22
- Species Reactivity
- Human
- Clone
- Inotuzumab
- Applications
- ELISA, FC, IP, FuncS, IF, Neut, ICC
Product Property
- Purification
- Protein A/G Purified
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Flow Cytometry, Immunoprecipitation, Functional Assay, Immunofluorescence, Neutralization, Immunocytochemistry.
Target
- Alternative Names
- SIGLEC2; SIGLEC-2; B-cell receptor CD22; B-lymphocyte cell adhesion molecule; BL-CAM; CD22 antigen; T-cell surface antigen Leu-14; sialic acid-binding Ig-like lectin 2
- Gene ID
- 933
- UniProt ID
- P20273
Related Resources
Please refer to Inotuzumab Ozogamicin Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Inotuzumab Ozogamicin.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Biparatopic Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-176 | Anti-Human CD22 Recombinant Antibody (TAB-176) | IF, IP, Neut, FuncS, ELISA, FC, ICC | IgG1 |
| TAB-1725CL | Human Anti-CD22 Recombinant Antibody (TAB-1725CL) | Block, Cyt, ELISA, FuncS | Humanized IgG |
| TAB-1729CL | Human Anti-CD22 Recombinant Antibody (TAB-1729CL) | IF, FC | Humanized IgG |
| TAB-002LC | Anti-Human CD22 Recombinant Antibody (VM1000) | FC, ELISA, SPR, FuncS | Humanized antibody |
| TAB-002LC-S(P) | Anti-Human CD22 Recombinant Antibody scFv Fragment (VM1000) | ELISA, SPR | Humanized antibody |
Customer Reviews and Q&As
Customer Review
Q&As
Good for Immunofluorescence
We conjugated Inotuzumab with PE and used it in immunofluorescence. It provided clear and specific staining of B cells. Highly recommend.
Scalability for Large Studies
Able to scale up usage for large cohort studies without compromising on performance. Supports extensive research applications.
Minimal Cross-reactivity
We tested Inotuzumab for cross-reactivity, and it showed minimal non-specific binding. Great for our human-specific studies.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-198, RRID: AB_3111867)
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For Research Use Only. Not For Clinical Use.
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