Anti-Human ERBB2 Recombinant Antibody (TAB-053)

Name: Anti-Human ERBB2 Recombinant Antibody (TAB-053)
SKU: TAB-053
Rating: 5 (1 reviews)

CAT#: TAB-053

Recombinant monoclonal antibody to ERBB2. It is a monoclonal antibody. The first of its class in a line of agents called "HER dimerization inhibitors". By binding to HER2, it inhibits the dimerization of HER2 with other HER receptors, which is hypothesized to result in slowed tumor growth.

Tested Data Gene Expression

SPR

Figure 1 Anti-Human ERBB2 Recombinant Antibody (TAB-053) in SPR

SPR assays of TAB-053.
The affinity of Anti-Human ERBB2 Antibody (TAB-053) was determined by SPR.
Captured TAB-053 on CM5 Chip via Anti-Human IgG (Fc) can bind Human ERBB2 protein with an affinity constant of 1.88 nM as determined in a SPR assay (Biacore 8K).

ELISA

Figure 2 Anti-ErbB2 Antibody (TAB-053) in ELISA

ELISA analysis of TAB-053 was performed by coating with human ErbB2 protein.

Figure 3 Anti-ErbB2 Antibody (TAB-053) in Dot Blot

Dot Blot analysis of TAB-053 was performed by coating with human ErbB2 protein.

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Subcellular Location Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1060/169_D12_1_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1338/if_selected.jpg

Normal Tissue Figure 3 High expression in esophagus

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1383/4682_B_5_1_val_selected.jpg

Normal Tissue Figure 4 IHC staining of human rectum

Immunohistochemical staining of human rectum shows weak cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/ihc_selected.jpg

Normal Tissue Figure 5 IHC staining of human urinary bladder

Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and membranous positivity in urothelial cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/ihc_selected.jpg

Normal Tissue Figure 6 Kidney

Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: Low Intensity: Moderate Quantity: <25%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/1054_A_7_5.jpg

Normal Tissue Figure 7 Breast

Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Myoepithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/45831_B_3_4.jpg

RNA Expression Figure 8 RNA cell line category: Group enriched (OE19, SK-BR-3)

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141736-ERBB2

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Specifications

Immunogen
The details of the immunogen for this antibody are not available.

Host Species
Mouse

Derivation
Humanized (from mouse)

Type
IgG1 - kappa

Specificity
Tested positive against native human antigen.

Species Reactivity
Human

Applications
FuncS, IF, Neut, ELISA, FC, IP, WB

Related Disease
Prostate cancers

Product Property

Purity
>95.0% as determined by Analysis by RP-HPLC & analysis by SDS-PAGE.

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
The ERBB2 antibody has been reported in applications of WB, Internalization, IF, Inhib.
Inhibition: In short-term experiments, trastuzumab (10 ng/μl), pertuzumab (10 ng/μl) and erlotinib (1 μM) were added to cells in starvation medium either alone or in combinations 1 h prior to growth-factor stimulation.

Target

Alternative Names
Pertuzumab;pertuzumab, Omnitarg;380610-27-5;rhuMAb 2C4;RG1273;pertuzumab;2C4;pertuzumab;Omnitarg;ERBB2;v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian);NGL, v erb b2 avian erythroblastic leukemia viral

Gene ID
2064

UniProt ID
P04626

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To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-053, RRID: AB_3111779)

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Related Diseases

Bladder Cancer

Non-small Cell Lung Cancer

Related Signaling Pathways

ErbB Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-053.pdf

MSDS

MSDS - TAB-053.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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CAT	Product Name	Application	Type
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HPAB-1112LY-S(P)	Human Anti-ERBB2 Recombinant Antibody; scFv Fragment (HPAB-1112LY-S(P))	ELISA	Human scFv
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CAT	Product Name	Application	Type
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CAT	Product Name	Application	Type
TAB-033CT-F(E)	Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (TAB-033CT-F(E))	ELISA, WB	Humanized Fab
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TAB-050CT-F(E)	Anti-Human HER2/neu Recombinant Antibody Fab Fragment (HuA21)	ELISA, WB	Humanized antibody

CAT	Product Name	Application	Type
TAB-040CT-F(E)	Anti-Human HER2/neu Recombinant Antibody Fab Fragment (C6.5)	ELISA, WB	Human antibody
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TAB-044CT-F(E)	Anti-Human HER2/neu Recombinant Antibody Fab Fragment (TAB-044CT-F(E))	WB	Human antibody
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CAT	Product Name	Application	Type
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CAT	Product Name	Application	Type
Gly-145LC	Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation)	ELISA	Human antibody
Gly-146LC	Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation)	ELISA	Human antibody
Gly-147LC	Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation)	ELISA	Human antibody
Gly-148LC	Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation)	ELISA	Human antibody
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CAT	Product Name	Application	Type
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CAT	Product Name	Application	Type
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CAT	Product Name	Application	Type
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CAT	Product Name	Application	Type
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CAT	Product Name	Application	Type
MOR-1178	Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone DS1178AB)	IHC-P, IHC-Fr	Rabbit IgG
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CAT	Product Name	Application	Type
HPAB-0091-FY-F(E)	Human Anti-ERBB2 Recombinant Antibody (clone MIL12); Fab Fragment	ELISA	Humanized Fab
HPAB-1129-FY-S(P)	Human Anti-ERBB2 Recombinant Antibody (clone Her2-S1R2A-CS-1F7); Fab Fragment	ELISA, WB, FC, IF, IP	Human Fab
HPAB-1130-FY-S(P)	Human Anti-ERBB2 Recombinant Antibody (clone Her2-S1R2A-CS-1D11); Fab Fragment	ELISA, WB, FC, IF, IP	Human Fab
HPAB-1131-FY-S(P)	Human Anti-ERBB2 Recombinant Antibody (clone Her2-S1R2C-CS-1D3); Fab Fragment	ELISA, WB, FC, IF, IP	Human Fab
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MHC-YF479	A*02:01/Human HER-2/neu (ILHNGAYSL) MHC Pentamer	FCM
MHC-YF480	A*02:01/Human HER-2/neu (CLTSTVQLV) MHC Pentamer	FCM
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CAT	Product Name	Application	Type
VS-0424-XY91	AbPlus™ Anti-ERBB2 Magnetic Beads (3.F2)	IP, Protein Purification
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CAT	Product Name	Application	Type
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CAT	Product Name	Application	Type
VS-0225-XY108	CytoStream™ Mouse Anti-ERBB2 Recombinant Antibody (clone 24D2)	FC	Mouse IgG1, kappa

CAT	Product Name	Application	Type
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CAT	Product Name	Application	Type
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CAT	Product Name	Application	Type
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