Human Anti-ERBB2 Recombinant Antibody (clone bH1); scFv Fragment

Name: Human Anti-ERBB2 Recombinant Antibody (clone bH1); scFv Fragment
SKU: PSBZ-041
Rating: 5 (1 reviews)

CAT#: PSBZ-041

Recombinant Human Antibody (bH1) scFv Fragment is specific to ERBB2, expressed in E. coli.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1060/169_D12_1_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1338/if_selected.jpg

Normal Tissue Figure 3 High expression in esophagus

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1383/4682_B_5_1_val_selected.jpg

Normal Tissue Figure 4 IHC staining of human rectum

Immunohistochemical staining of human rectum shows weak cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/ihc_selected.jpg

Normal Tissue Figure 5 IHC staining of human urinary bladder

Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and membranous positivity in urothelial cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/ihc_selected.jpg

Normal Tissue Figure 6 Kidney

Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: Low Intensity: Moderate Quantity: <25%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/1054_A_7_5.jpg

Normal Tissue Figure 7 Breast

Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Myoepithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/45831_B_3_4.jpg

RNA Expression Figure 8 RNA cell line category: Group enriched (OE19, SK-BR-3)

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141736-ERBB2

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Specifications

Immunogen
Human erb-b2 receptor tyrosine kinase 2

Host Species
Human

Type
Human scFv

Specificity
Human ERBB2

Species Reactivity
Human

Clone
bH1

Applications
WB, IF, FuncS

Product Property

Purity
>95% as determined by SDS-PAGE and HPLC analysis

Concentration
Please refer to the vial label for the specific concentration.

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
ERBB2; erb-b2 receptor tyrosine kinase 2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu; receptor tyrosine-protein kinase erbB-2; herstatin; p185erbB2; proto-oncogene Neu; c-erb B2/neu protein; proto-oncogene c-ErbB-2; metastatic lymph node gene 19 protein; human epidermal growth factor receptor 2; neuro/glioblastoma derived oncogene homolog; tyrosine kinase-type cell surface receptor HER2; neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog

Gene ID
2064

UniProt ID
P04626

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Related Diseases

Bladder Cancer

Non-small Cell Lung Cancer

Related Signaling Pathways

ErbB Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - PSBZ-041.pdf

MSDS

MSDS - PSBZ-041.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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CAT
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