Anti-Human HER2/neu Recombinant Antibody (ChA21)
CAT#: TAB-049CT
Recombinant monoclonal antibody to HER2/neu. This antibody intended for the prophylaxis and treatment of HER2-positive breast cancer.
Published Data
Gene Expression
IHC
Figure 1 SK-OV-3 invasion after incubation with chA21 for 24h(x400).
The invaded cells were counted on the underneath of ECM-coated Millicell membrane, chA21 (6μg/ml).
Gao, Y., Wu, Q., Wu, Z. S., Zhang, G. H., & Zhang, A. L. (2011). Effects of an engineered anti-HER2 antibody chA21 on invasion of human ovarian carcinoma cell in vitro. Chinese Journal of Cancer Research, 23(2), 147-152.
IHC
Figure 2 Growth inhibition on tumor xenografts by chA21.
ErbB2-positive expression on BT-474 and SKOV-3 tumor xenografts conWrmed by IHC method. Scale bar 500 μm.
Shen, G., Huang, H., Zhang, A., Zhao, T., Hu, S., Cheng, L., ... & Song, L. (2011). In vivo activity of novel anti-ErbB2 antibody chA21 alone and with Paclitaxel or Trastuzumab in breast and ovarian cancer xenograft models. Cancer immunology, immunotherapy, 60(3), 339-348.
Inhib
Figure 3 Growth inhibition on tumor xenografts by chA21.
A dose-dependent response induced by chA21 on both BT-474 and SKOV-3 xenografts. chA21 was given i.v. twice a week for total 24 days at doses of 20, 30 and 40 mg/kg. *p < 0.01 compared with control. **p < 0.01 compared with 20 mg/kg chA21 group. Results are given as representative of two independent experiments.
Shen, G., Huang, H., Zhang, A., Zhao, T., Hu, S., Cheng, L., ... & Song, L. (2011). In vivo activity of novel anti-ErbB2 antibody chA21 alone and with Paclitaxel or Trastuzumab in breast and ovarian cancer xenograft models. Cancer immunology, immunotherapy, 60(3), 339-348.
WB
Figure 4 Growth inhibition on tumor xenografts by chA21.
Dose-dependent down-regulation of ErbB2 receptor on both BT-474 and SKOV-3 xenografts by chA21. By the end of in vivo experiment, tumors were excised, lysed and subjected to western blots with specific antibodies against ErbB2 ECD (Ab-20) and GAPDH.
Shen, G., Huang, H., Zhang, A., Zhao, T., Hu, S., Cheng, L., ... & Song, L. (2011). In vivo activity of novel anti-ErbB2 antibody chA21 alone and with Paclitaxel or Trastuzumab in breast and ovarian cancer xenograft models. Cancer immunology, immunotherapy, 60(3), 339-348.
Activ
Figure 5 Increased number and activation of NK cells by chA21.
Nude mice bearing BT-474 xenografts were treated i.v. with indicated drug alone or combinations. At the end of in vivo experiment, the total numbers of NK cells (DX5+ CD3- cells) in peripheral blood and the percentages of NK cells in lymphocytes were determined by fow cytometry.
Shen, G., Huang, H., Zhang, A., Zhao, T., Hu, S., Cheng, L., ... & Song, L. (2011). In vivo activity of novel anti-ErbB2 antibody chA21 alone and with Paclitaxel or Trastuzumab in breast and ovarian cancer xenograft models. Cancer immunology, immunotherapy, 60(3), 339-348.
FC
Figure 6 Increased number and activation of NK cells by chA21.
NK cells were evaluated for CD69 and NKG2D expression by fow cytometry. Histograms show CD69 and NKG2D expression on this cell subset, and mean fuorescence intensity (MFI) denotes expression level. Tra Trastuzumab, PTX Paclitaxel. Results were presented as the average ± SD. Similar results were obtained in mice bearing SKOV-3 xenografts.
Shen, G., Huang, H., Zhang, A., Zhao, T., Hu, S., Cheng, L., ... & Song, L. (2011). In vivo activity of novel anti-ErbB2 antibody chA21 alone and with Paclitaxel or Trastuzumab in breast and ovarian cancer xenograft models. Cancer immunology, immunotherapy, 60(3), 339-348.
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1060/169_D12_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1338/if_selected.jpg
Normal Tissue
Figure 3 High expression in esophagus
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1383/4682_B_5_1_val_selected.jpg
Normal Tissue
Figure 4 IHC staining of human rectum
Immunohistochemical staining of human rectum shows weak cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/ihc_selected.jpg
Normal Tissue
Figure 5 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and membranous positivity in urothelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/ihc_selected.jpg
Normal Tissue
Figure 6 Kidney
Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: Low Intensity: Moderate Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/1054_A_7_5.jpg
Normal Tissue
Figure 7 Breast
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Myoepithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/45831_B_3_4.jpg
RNA Expression
Figure 8 RNA cell line category: Group enriched (OE19, SK-BR-3)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141736-ERBB2
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Specifications
- Host Species
- Human
- Type
- Chimeric antibody (mouse/human)
- Specificity
- Human
- Species Reactivity
- Human
- Clone
- ChA21
- Applications
- IHC, Inhib, WB, Activ, FC
- Related Disease
- HER2-positive breast cancer
Applications
- Application Notes
- The HER2/neu antibody has been reported in applications of Immunohistochemistry, Inhibition, Western Blot, Activation, Flow Cytometry.
For Western Blot: Excised BT-474 or SKOV-3 tumors were frozen in liquid nitrogen immediately after dissection. Tumors were homogenized and lysed in RIPA lysis buVer (10 mM Tris–HCl pH 7.4, 150 mM NaCl, 1% sodium deoxycholate, 1% Triton X-100, 1% (v/v) Np-40, 0.1% SDS, 30 mM NaF, 1 mM Na₃VO₄, 1% Aprotinin, 2 mM EDTA) for 10 min. The lysates were centrifuged at 12,000 g for 10 min and the cleared supernatants were collected. Protein concentration in the supernatants was measured by BCA protein assay kit, equal amount of total proteins per well were loaded and separated in SDS-PAGE gels, and then transferred onto nitrocellulose membranes. After blocking with 5% non-fat milk in TBS +0.1% Tween-20 for 2 h at room temperature, the membranes were incubated with appropriate primary antibodies at 4°C overnight followed by probing with secondary antibodies for 2 h at room temperature. Immunoreactive bands were visualized with chemiluminescence (ECL) reagents. The band densities were scanned for densitometric analysis using ImageJ 1.42 software.
Target
- Alternative Names
- ERBB2; erb-b2 receptor tyrosine kinase 2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu; receptor tyrosine-protein kinase erbB-2; herstatin; p185erbB2; proto-oncogene Neu; c-erb B2/neu protein; proto-oncogene c-ErbB-2; metastatic lymph node gene 19 protein; human epidermal growth factor receptor 2; neuro/glioblastoma derived oncogene homolog; tyrosine kinase-type cell surface receptor HER2; neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog;
- Gene ID
- 2064
- UniProt ID
- P04626
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Related Diseases
Bladder Cancer
Non-small Cell Lung Cancer
Related Signaling Pathways
ErbB Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| IAB-B008(A) | Recombinant Anti-human ERBB2 Intrabody [(D-Arg)9] | IF, FC, FuncS | scFv-(D-Arg)9 |
| IAB-B008(G) | Recombinant Anti-human ERBB2 Intrabody [+36 GFP] | WB, ICC, FuncS | scFv-(+36GFP) |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-761 | Anti-ERBB2 Recombinant Antibody (Margetuximab) | IF, IP, Neut, FuncS, ELISA, FC, WB | IgG1 - kappa |
| TAB-049CT-S(P) | Anti-Human HER2/neu Recombinant Antibody scFv Fragment (ChA21) | WB | Chimeric antibody (mouse/human) |
| TAB-049CT-F(E) | Anti-Human HER2/neu Recombinant Antibody Fab Fragment (ChA21) | WB | Chimeric antibody (mouse/human) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-H70 | Anti-Human ERBB2 Recombinant Antibody (TAB-H70) | ELISA, IP, FC, FuncS, Neut, IF, ICC | IgG1 - kappa |
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| TAB-033CT | Human Anti-ERBB2 Recombinant Antibody (TAB-033CT) | ELISA, WB | Human IgG1, κ |
| TAB-038CT | Human Anti-ERBB2 Recombinant Antibody (TAB-038CT) | FuncS, FC | Humanized IgG1 |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G022E | Anti-ERBB2 immunotoxin 4D5 (scFv)-PE | Cytotoxicity assay, Function study | |
| AGTO-G022D | Anti-ERBB2 immunotoxin 4D5 (scFv)-DT | Cytotoxicity assay, Function study | |
| AGTO-G022G | Anti-ERBB2 immunotoxin 4D5 (scFv)-Gel | Cytotoxicity assay, Function study | |
| AGTO-G022R | Anti-ERBB2 immunotoxin 4D5 (scFv)-RTA | Cytotoxicity assay, Function study | |
| AGTO-G022S | Anti-ERBB2 immunotoxin 4D5 (scFv)-Sap | Cytotoxicity assay, Function study |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBZ-041 | Human Anti-ERBB2 Recombinant Antibody (clone bH1); scFv Fragment | WB, IF, FuncS | Human scFv |
| PFBC-157 | Human Anti-ERBB2 Recombinant Antibody (clone F0178C1); Fab Fragment | Neut | Human Fab |
| FAMAB-0132CQ-S(P) | Mouse Anti-ERBB2 Recombinant Antibody (clone SER4); scFv Fragment | ELISA | Mouse scFv |
| HPAB-0091-FY-S(P) | Human Anti-ERBB2 Recombinant Antibody (clone MIL12); scFv Fragment | ELISA | Humanized scFv |
| HPAB-0733LY-S(P) | Human Anti-ERBB2 Recombinant Antibody (clone AH06); scFv Fragment | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0191CL | Human Anti-ERBB2 Recombinant Antibody (TAB-0191CL) | ELISA, Cyt, Internalization | Human IgG |
| TAB-0192CL | Human Anti-ERBB2 Recombinant Antibody (TAB-0192CL) | ELISA, ADCC, Cyt, Internalization | Human IgG |
| TAB-0193CL | Human Anti-ERBB2 Recombinant Antibody (TAB-0193CL) | ELISA, ADCC, Cyt, Internalization | Human IgG |
| TAB-0194CL | Human Anti-ERBB2 Recombinant Antibody (TAB-0194CL) | ELISA, ADCC, Cyt, Internalization | Human IgG |
| PABX-081-S (P) | Recombinant Human Anti-HER2 Antibody scFv Fragment (Gr3) | WB, ELISA, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-034CT | Anti-Human HER2/neu Recombinant Antibody (TAB-034CT) | WB | |
| TAB-270CQ | Mouse Anti-ERBB2 Recombinant Antibody (TAB-270CQ) | WB, Inhib | Mouse IgG |
| TAB-270CQ-S(P) | Mouse Anti-ERBB2 Recombinant Antibody; scFv Fragment (TAB-270CQ-S(P)) | WB, Inhib | Mouse scFv |
| TAB-270CQ-F(E) | Mouse Anti-ERBB2 Recombinant Antibody; Fab Fragment (TAB-270CQ-F(E)) | WB, Inhib | Mouse Fab |
| PABX-082-S (P) | Recombinant Human Anti-HER2 Antibody scFv Fragment (Gr6) | WB, ELISA, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-024LC | Recombinant Anti-Human ERBB2 Antibody (Fab glycosylation) | ELISA | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-117LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Humanized antibody |
| Gly-145LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
| Gly-146LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
| Gly-147LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
| Gly-148LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-177LC | Recombinant Anti-Human ERBB2 Antibody (Non-glycosylated) | ELISA | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0195MZ | Chicken Anti-ERBB2 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-737CQ | Mouse Anti-ERBB2 Recombinant Antibody (clone 7.16.4) | Block, IP, IF, FC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1178 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone DS1178AB) | IHC-P, IHC-Fr | Rabbit IgG |
| MOR-4586 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone TH99DS) | WB | Rabbit IgG |
| MOR-4587 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone TH100DS) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0098-YC-F(E) | Mouse Anti-ERBB2 Recombinant Antibody (clone E402); Fab Fragment | Cyt | Mouse Fab |
| FAMAB-0099-YC-F(E) | Human Anti-ERBB2 Recombinant Antibody (clone CH402); Fab Fragment | Cyt | Human Fab |
| FAMAB-0132CQ-F(E) | Mouse Anti-ERBB2 Recombinant Antibody (clone SER4); Fab Fragment | ELISA | Mouse Fab |
| HPAB-0193-WJ-F(E) | Mouse Anti-ERBB2 Recombinant Antibody (clone 6H8); Fab Fragment | ELISA | Mouse Fab |
| HPAB-1129-FY-S(P) | Human Anti-ERBB2 Recombinant Antibody (clone Her2-S1R2A-CS-1F7); Fab Fragment | ELISA, WB, FC, IF, IP | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0010-FY | Human Anti-ERBB2 Recombinant Antibody (HPAB-0010-FY) | ELISA, FC, ADCC | Human IgG1, κ |
| HPAB-0011-FY | Mouse Anti-ERBB2 Recombinant Antibody (HPAB-0011-FY) | Inhib, ADCC, FuncS | Mouse IgG1, κ |
| HPAB-0608LY | Human Anti-ERBB2 Recombinant Antibody (HPAB-0608LY) | ELISA, Inhib, WB, FC | Humanized IgG1 |
| HPAB-0609LY | Human Anti-ERBB2 Recombinant Antibody (HPAB-0609LY) | ELISA, Inhib | Humanized IgG1 |
| HPAB-0610LY | Human Anti-ERBB2 Recombinant Antibody (HPAB-0610LY) | ELISA, Inhib | Humanized IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-468CQ | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-468CQ) | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| AFC-TAB-053 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-053) | FuncS, IF, Neut, ELISA, FC, IP | ADCC enhanced antibody |
| AFC-TAB-761 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-761) | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| AFC-TAB-005 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-005) | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF049 | DRB1*01:01/Human HER2 (KVPIKWMALESILRRRF) MHC Monomer | MHC Multimer | |
| MHC-YF055 | DRB1*04:01/Human HER2 (KVPIKWMALESILRRRF) MHC Monomer | MHC Multimer | |
| MHC-YF479 | A*02:01/Human HER-2/neu (ILHNGAYSL) MHC Pentamer | FCM | |
| MHC-YF480 | A*02:01/Human HER-2/neu (CLTSTVQLV) MHC Pentamer | FCM | |
| MHC-YF481 | A*24:02/Human HER-2/neu (TYLPTNASL) MHC Pentamer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY91 | AbPlus™ Anti-ERBB2 Magnetic Beads (3.F2) | IP, Protein Purification | |
| VS-0724-YC1516 | AbPlus™ Anti-Erbb2 Magnetic Beads (VS-0724-YC1516) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY24 | Human Anti-ERBB2 (clone 1.18.1) scFv-Fc Chimera | FC, BI | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY108 | CytoStream™ Mouse Anti-ERBB2 Recombinant Antibody (clone 24D2) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC430 | Recombinant Anti-ERBB2 Vesicular Antibody, EV Displayed (VS-0425-YC430) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2335 | Anti-ERBB2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2336 | Anti-Mouse ERBB2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC3 | Recombinant Anti-ERBB2 Biparatopic Antibody, Symmetric scFv-Fab | ELISA | Symmetric scFv-Fab |
| VS-0525-YC6 | Recombinant Anti-ERBB2 Biparatopic Antibody, Asymmetric hetero Fab | ELISA | Asymmetric hetero Fab |
| VS-0525-YC7 | Recombinant Anti-ERBB2 Biparatopic Antibody, Asymmetric hetero HC-LC | ELISA, Inhib | Asymmetric hetero HC, common LC |
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