Anti-HER2/neu Antibody-Cytokine Fusion Protein, RLI-scFv (trastuzumab) (CAT#: ACFP-SH152)
This antibody-cytokine fusion protein was achieved by conjugating/fusing the Anti-HER2/neu scFv to RLI. It was expressed in CHO and purified with affinity chromatography. The immunocytokine retains the ability to bind the HER2/neu as well as the biological activity of RLI. The goal of this antibody-cytokine fusion protein to cancer therapy is to concentrate the cytokine in the tumor microenvironment and in so doing directly enhance the tumoricidal effect of the antibody and/or enhance the host immune response (T-cell, B-cell or NK) against the tumor. This immunocytokine was designed for treating HER-2/neu over-expressing cancer.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1060/169_D12_1_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1060/169_D12_1_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1338/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1338/if_selected.jpg
Figure 3 High expression in esophagus
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1383/4682_B_5_1_val_selected.jpg
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1383/4682_B_5_1_val_selected.jpg
Figure 4 IHC staining of human rectum
(Immunohistochemical staining of human rectum shows weak cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/ihc_selected.jpg
(Immunohistochemical staining of human rectum shows weak cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/ihc_selected.jpg
Figure 5 IHC staining of human urinary bladder
(Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and membranous positivity in urothelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/ihc_selected.jpg
(Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and membranous positivity in urothelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/ihc_selected.jpg
Figure 6 Kidney
(Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/1054_A_7_5.jpg
(Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/1054_A_7_5.jpg
Figure 7 Breast
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Myoepithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/45831_B_3_4.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Myoepithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/45831_B_3_4.jpg
Figure 8 RNA cell line category: Group enriched (OE19, SK-BR-3)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141736-ERBB2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141736-ERBB2
Specifications
- Target
- HER2/neu
- Molecule Class
- RLI-IgG1
- Species Reactivity
- Human
- Expression Host
- CHO
- Clone
- trastuzumab
- Related Disease
- HER-2/neu over-expressing cancer
- Cytokine 1
- RLI
- Cytokines 1 Description
- ATP binding cassette E1 (ABCE1, also RNase L inhibitor) is an ATPase found in humans involved in viral assembly. It is a member of the ATP-binding cassette (ABC) transporters superfamily and OABP subfamily. ABCE1 inhibits the action of ribonuclease L. Ribonuclease L normally binds to 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) and inhibits the interferon-regulated 2-5A/RNase L pathway, which is used by viruses. ABCE1 heterodimerize with ribonuclease L and prevents its interaction with 2-5A, antagonizing the anti-viral properties of ribonuclease L, and allow the virus to synthesize viral proteins. It has also been implicated to have an effect in tumor cell proliferation and antiapoptosis.
- Cytokine 1 Species
- Human
- Applications
- This product can be used therapeutically to deliver biologically active ligands to a desired tissue.
Target
- Alternative Names
- ERBB2; erb-b2 receptor tyrosine kinase 2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu; receptor tyrosine-protein kinase erbB-2; herstatin; p185erbB2; proto-oncogene Neu; c-erb B2/neu protein; proto-oncogene c-ErbB-2; metastatic lymph node gene 19 protein; human epidermal growth factor receptor 2; neuro/glioblastoma derived oncogene homolog; tyrosine kinase-type cell surface receptor HER2; neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2; neuro/glioblastoma derived oncogene homolog
- Gene ID
- 2064
- UniProt ID
- P04626
Related Resources
Bladder Cancer
Non-small Cell Lung Cancer
ErbB Signaling Pathway
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-059 | Recombinant Anti-Human ERBB2 VHH Single Domain Antibody | IHC, FC, CA, FuncS | Llama VHH |
| PNBL-066 | Recombinant Anti-HER2 VHH Single Domain Antibody (PNBL-066) | SPR | Llama VHH |
| PNBL-067 | Recombinant Anti-HER2 VHH Single Domain Antibody (PNBL-067) | SPR | Llama VHH |
| PNBL-069 | Recombinant Anti-HER2 VHH Single Domain Antibody (PNBL-069) | ELISA | Llama VHH |
| TAB-032CT | Anti-Human HER2/neu Single Domain Antibody, Research Grade Biosimilar | WB | Single domain antibody |
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