Anti-HIV-1 gp120 Recombinant Antibody (G3-4)
CAT#: MRO-150LC
This product is a recombinant mouse anti-HIV-1 monoclonal antibody (G3-4). G3-4 specifically binds to gp120 and can be potentially used in the treatment studies of acquired immunodeficiency syndrome (AIDS), a condition in humans characterized by clinical features including wasting syndromes, central nervous system degeneration and profound immunosuppression that results in life-threatening opportunistic infections and malignancies.
Published Data
Inhib
Figure 1 Inhibitory activity of antibodies on sCD4-gpl2O binding. mAbs were G3-4 (v), 15e (A), G3-519 (o), and BAT123 (o).
Another discontinuous epitope on glycoprotein gpl20 that is important in human immunodeficiency virus type 1 neutralization is identified by a monoclonal antibody
FC
Figure 2 Reactivity of mAb G3-4 with two HIV-1 isolates (Ille and RF) by radioimmunoprecipitation assay (A) and immunofluorescence and analyzed by flow cytometry (B).
Another discontinuous epitope on glycoprotein gpl20 that is important in human immunodeficiency virus type 1 neutralization is identified by a monoclonal antibody
Neut
Figure 3 Neutralizing activity of mAb G3-4 against laboratory isolates: "IB (o), RF (A), and MN (0).
Another discontinuous epitope on glycoprotein gpl20 that is important in human immunodeficiency virus type 1 neutralization is identified by a monoclonal antibody
RIPA
Figure 4 Radioimmunoprecipitation assay of mAb G3-4 reactivity with HIV-1 lysate reduced with dithiothreitol.
Another discontinuous epitope on glycoprotein gpl20 that is important in human immunodeficiency virus type 1 neutralization is identified by a monoclonal antibody
DB
Figure 5 Slot-blot immunoassays of mAbs G3-4, I5e, and BAT123 reactivity with the III gpl20 in the presence of (+) or the absence of (-) dithiothreitol (DTT) reduction. PC, positive-control human serum.
Another discontinuous epitope on glycoprotein gpl20 that is important in human immunodeficiency virus type 1 neutralization is identified by a monoclonal antibody
Inhib
Figure 6 Inhibitory activity of mAbs G34 (A), BAT123 (o), G3-519 (e), and 15e (o) on the binding of biotinylated mAb G3-4 to captured 111B gpl20.
Another discontinuous epitope on glycoprotein gpl20 that is important in human immunodeficiency virus type 1 neutralization is identified by a monoclonal antibody
ELISA
Figure 9 ELISA binding curves of select anti-gp120 Abs against full-length and loop-deleted constructs of gp140JR-FL.
Full-length (diamonds), V1-deleted (squares), V2-deleted (triangles), and V3-deleted (x) gp140JR-FL were captured via the C-terminal region of gp120 with the sheep Ab D7324 and probed with pooled HIVIG, 2G12, Fab AH48, G3-4, G3-519, G3-42, G3-299, and 4KG5. The detection reagents were ABTS for 4KG5 and AP staining solution for all other Abs.
A Novel Human Antibody against Human Immunodeficiency Virus Type 1 gp120 Is V1, V2, and V3 Loop Dependent and Helps Delimit the Epitope of the Broadly Neutralizing Antibody Immunoglobulin G1 b12
ELISA
Figure 10 ELISA inhibition curves of 4KG5 with various MAbs against gp120JR-FL.
A concentration of 4KG5 previously determined to result in approximately 75% of the maximal signal (∼0.3 μg/ml) was coincubated with various concentrations of b12 Fab (squares), b12 IgG (open diamonds), b6 Fab (circles), sCD4 (triangle), AH48 Fab (filled diamonds), or G3-4 IgG (X), and the level of 4KG5 binding to immobilized gp̶¹²⁰JR-FL was measured via the hexahistidine tag of 4KG5 by using the India-HIS probe (Pierce) as a secondary reagent.
A Novel Human Antibody against Human Immunodeficiency Virus Type 1 gp120 Is V1, V2, and V3 Loop Dependent and Helps Delimit the Epitope of the Broadly Neutralizing Antibody Immunoglobulin G1 b12
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Specifications
- Host Species
- Mouse
- Type
- Mouse antibody
- Antibody Isotype
- IgG
- Specificity
- HIV-1
- Clone
- G3-4
- Applications
- ELISA, Inhib, FC, Neut, RIPA, DB, IP
- Related Disease
- AIDS
Applications
- Application Notes
- The HIV-1 gp120 antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Inhibition, Flow Cytometry, Neutralization, RImmunoprecipitationA, Dot Blot, Immunoprecipitation.
Target
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Strong Binding to gp120
This recombinant mouse antibody, clone G3-4, exhibited specific binding to HIV-1 gp120 in our ELISA setups. It was essential for our research into viral envelope glycoproteins. The antibody performed consistently, and the data generated was reliable for our neutralization studies.
Versatile Research Tool
We appreciated the versatility of this product, using it successfully in both flow cytometry and immunoprecipitation assays. It is a high-quality reagent that has supported our investigation into gp120 interactions. The documentation provided was helpful for setting up our experiments.
Q&As
-
What is the specificity and production host of this antibody?
A: This is a recombinant mouse anti-HIV-1 monoclonal antibody (clone G3-4). It specifically binds to gp120, the envelope glycoprotein of HIV-1. The antibody is produced in CHO cells.
-
What are the recommended applications for this product?
A: This HIV-1 gp120 antibody (G3-4) has been reported for use in Enzyme-linked Immunosorbent Assay (ELISA), Inhibition, Flow Cytometry (FC), Neutralization (Neut), RIPA, Dot Blot (DB), and Immunoprecipitation (IP).
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# MRO-150LC, RRID: AB_3714056)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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COA
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