Anti-HIV-1 gp120 Recombinant Antibody (G3-4) (CAT#: MRO-150LC)

Figure 1 Inhibitory activity of antibodies on sCD4-gpl2O binding. mAbs were G3-4 (v), 15e (A), G3-519 (o), and BAT123 (o).

Another discontinuous epitope on glycoprotein gpl20 that is important in human immunodeficiency virus type 1 neutralization is identified by a monoclonal antibody

Figure 2 Reactivity of mAb G3-4 with two HIV-1 isolates (Ille and RF) by radioimmunoprecipitation assay (A) and immunofluorescence and analyzed by flow cytometry (B).

Another discontinuous epitope on glycoprotein gpl20 that is important in human immunodeficiency virus type 1 neutralization is identified by a monoclonal antibody

Figure 3 Neutralizing activity of mAb G3-4 against laboratory isolates: "IB (o), RF (A), and MN (0).

Another discontinuous epitope on glycoprotein gpl20 that is important in human immunodeficiency virus type 1 neutralization is identified by a monoclonal antibody

Figure 4 Radioimmunoprecipitation assay of mAb G3-4 reactivity with HIV-1 lysate reduced with dithiothreitol.

Another discontinuous epitope on glycoprotein gpl20 that is important in human immunodeficiency virus type 1 neutralization is identified by a monoclonal antibody

Figure 5 Slot-blot immunoassays of mAbs G3-4, I5e, and BAT123 reactivity with the III gpl20 in the presence of (+) or the absence of (-) dithiothreitol (DTT) reduction. PC, positive-control human serum.

Another discontinuous epitope on glycoprotein gpl20 that is important in human immunodeficiency virus type 1 neutralization is identified by a monoclonal antibody

Figure 6 Inhibitory activity of mAbs G34 (A), BAT123 (o), G3-519 (e), and 15e (o) on the binding of biotinylated mAb G3-4 to captured 111B gpl20.

Another discontinuous epitope on glycoprotein gpl20 that is important in human immunodeficiency virus type 1 neutralization is identified by a monoclonal antibody

Figure 7 Precipitation of the WT and DV3 mutant envelope glycoproteins by gp120-specific mAbs.

COS-1 cell transfectants expressing either WT or DV3 gp120 were lysed in NP-40 buffer and precipitated with the indicated mAbs in the presence or absence of 50 mg/ml of CRDS.

Role of the V2, V3, and CD4-Binding Domains of GP120 in Curdlan Sulfate Neutralization Sensitivity of HIV-1 during Infection of T Lymphocytes

Figure 8 Immunoprecipitation of envelope glycoproteins of HIV-1 clones.

The radiolabeled cell lysates were coprecipitated with anti-MHC class I mAb W6/32 to ensure loading equivalent amounts of protein. Results are representative of three separate experiments.

Role of the V2, V3, and CD4-Binding Domains of GP120 in Curdlan Sulfate Neutralization Sensitivity of HIV-1 during Infection of T Lymphocytes

Figure 9 ELISA binding curves of select anti-gp120 Abs against full-length and loop-deleted constructs of gp140JR-FL.

Full-length (diamonds), V1-deleted (squares), V2-deleted (triangles), and V3-deleted (x) gp140JR-FL were captured via the C-terminal region of gp120 with the sheep Ab D7324 and probed with pooled HIVIG, 2G12, Fab AH48, G3-4, G3-519, G3-42, G3-299, and 4KG5. The detection reagents were ABTS for 4KG5 and AP staining solution for all other Abs.

A Novel Human Antibody against Human Immunodeficiency Virus Type 1 gp120 Is V1, V2, and V3 Loop Dependent and Helps Delimit the Epitope of the Broadly Neutralizing Antibody Immunoglobulin G1 b12

Figure 10 ELISA inhibition curves of 4KG5 with various MAbs against gp120JR-FL.

A concentration of 4KG5 previously determined to result in approximately 75% of the maximal signal (∼0.3 μg/ml) was coincubated with various concentrations of b12 Fab (squares), b12 IgG (open diamonds), b6 Fab (circles), sCD4 (triangle), AH48 Fab (filled diamonds), or G3-4 IgG (X), and the level of 4KG5 binding to immobilized gp̶¹²⁰JR-FL was measured via the hexahistidine tag of 4KG5 by using the India-HIS probe (Pierce) as a secondary reagent.

A Novel Human Antibody against Human Immunodeficiency Virus Type 1 gp120 Is V1, V2, and V3 Loop Dependent and Helps Delimit the Epitope of the Broadly Neutralizing Antibody Immunoglobulin G1 b12