Anti-HIV-1 gp120 Recombinant Antibody (GP68) (CAT#: MRO-156LC)

This product is a recombinant human anti-HIV-1 monoclonal antibody. GP68 specifically binds to gp120 and can be potentially used in the treatment of acquired immunodeficiency syndrome (AIDS), a condition in humans characterized by clinical features including wasting syndromes, central nervous system degeneration and profound immunosuppression that results in life-threatening opportunistic infections and malignancies.

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Inhib

Figure 1 Inhibition of CD4 binding to gp120.

Figure 1 Inhibition of CD4 binding to gp120.

ELISAs were carried out to confirm that CD4bs mabs blocked sCD4 binding to gp120. For human mabs (except for gp68), serial (0-8 μg/ml) antibody dilutions were added to saturating immobilized amounts of IIIB gp120 (Immunodiagnostics Inc.) captured with a sheep anti-gp120 C-terminal peptide (Alto Bio Reagents Inc.). Appropriate sCD4 dilutions were then added and bound sCD4 detected using mouse mab, OKT4, followed by an anti-mouse IgG-HRP conjugate (Thermo Scientific Inc.). Mabs gp68 and ICR39.13 were in the form of hybridoma culture supernatant and were added at 1:2 to 1:16 dilutions. ICR39.13 is a rat antibody and was evaluated by competition with PRO542 (CD4-IgG) [17], followed by and ant-human IgG-HRP conjugate. All mabs designated as binding the CD4bs blocked sCD4 binding to recombinant gp120. Most blocked sCD4 highly efficiently, although neither VRC03 nor F105 was particularly effective. Since the structures of VRC03 and F105 bound to gp120 irrevocably prove their specificity for the CD4bs [14,18], the reasons for weaker sCD4 inhibition are unclear. We also tested the CD4i mab, 17b, as a control. As expected, this mab failed to block sCD4 binding to gp120.

The W100 pocket on HIV-1 gp120 penetrated by b12 is not a target for other CD4bs monoclonal antibodies


Specifications

  • Host Species
  • Human
  • Type
  • Human antibody
  • Specificity
  • HIV-1
  • Clone
  • GP68
  • Applications
  • ELISA, Inhib
  • Related Disease
  • AIDS

Applications

  • Application Notes
  • The HIV-1 gp120 antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Inhibition.

Target

  • Alternative Names
  • Human immunodeficiency virus type 1; HIV-1; Envelope glycoprotein GP120; gp120; HIV envelope

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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See other products for "Clone GP68"

See other products for "HIV-1 gp120"

Chimeric Antibody

CAT Product Name Application Type
MRO-135LC Anti-HIV-1 gp120 Recombinant Antibody (CAG1-51-4) ELISA, WB, Neut Chimeric antibody (mouse/human)
MRO-135LC-S(P) Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (CAG1-51-4) ELISA, WB Chimeric antibody (mouse/human)
MRO-135LC-F(E) Anti-HIV-1 gp120 Recombinant Antibody Fab Fragment (CAG1-51-4) ELISA, WB Chimeric antibody (mouse/human)

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-4220 Rabbit Anti-HIV-1 gp120 Recombinant Antibody (clone SI299DS) WB, ELISA Rabbit IgG

Humanized Antibody

CAT Product Name Application Type
PABX-101-S(P) Human Anti-HIV-1 gp120 Recombinant Antibody (clone PG9); scFv Fragment WB, ELISA, FuncS Human scFv

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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