Recombinant Mouse Anti-IAV HA Antibody (C179) (CAT#: PABL-209)

Figure 1 The ELISA results of top 12 phage clones with higher C179/BSA ELISA signal ratio from Ph.D.-7, Ph.D.-12 and Ph.D.-C7C peptide phage-display libraries.

a: Phage clones from Ph.D.-7 peptide phage-display library. b: Phage clones from Ph.D.-12 peptide phage-display library; c: Phage clones from Ph.D.-C7C peptide phage-display library.

Zhong, Y., Cai, J., Zhang, C., Xing, X., Qin, E., He, J.,... & Song, H. (2011). Mimotopes selected with neutralizing antibodies against multiple subtypes of influenza A. Virology journal, 8(1), 542.

Figure 2 The in vitro binding activities of recombinant multi-mimotope.

Zhong, Y., Cai, J., Zhang, C., Xing, X., Qin, E., He, J.,... & Song, H. (2011). Mimotopes selected with neutralizing antibodies against multiple subtypes of influenza A. Virology journal, 8(1), 542.

Figure 3 Evaluation of ELISA. Group 1 HA stalk antigen coated onto the wells of an ELISA plate maintained important conformational epitopes.

(A to C) ELISA binding of group 1 HA stalk-binding monoclonal antibodies (in picograms per 50 μl) was measured using serial dilutions of monoclonal antibodies CR6261 (A), C179 (B), and 70-1F02 (C). (D) H1 HA globular head-binding monoclonal antibody EM4CO4 was used as a negative control. Anti-HA stalk serum titers measured from patient samples in this study showed strong positive correlation with the level of inhibition to potent group 1 HA stalk-binding monoclonal antibodies. Samples were ranked by the anti-HA stalk antibody titer and the percent inhibition to stalk-binding monoclonal antibodies for Spearman's rank correlation analysis. (E to G) Increasing anti-HA stalk titer showed a strong and significant tendency of increasing inhibition of CR6261 (E), C179 (F), and 70-1F02 (G) binding.

Park, J. K., Han, A., Czajkowski, L., Reed, S., Athota, R., Bristol, T.,... & Memoli, M. J. (2018). Evaluation of preexisting anti-hemagglutinin stalk antibody as a correlate of protection in a healthy volunteer challenge with influenza A/H1N1pdm virus.MBio, 9(1), e02284-17.

Figure 1 Anti-Influenza A Virus HA Recombinant Antibody (PABL-209) in SDS-PAGE

SDS-PAGE analysis of PABL-209 in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 135-180 kDa.

Figure 2 Anti-Influenza A Virus HA Recombinant Antibody (PABL-209) in SEC-HPLC

SEC-HPLC analysis of PABL-209 shows the purity is>95%.

Figure 3 Anti-Influenza A Virus HA Recombinant Antibody (PABL-209) in ELISA

ELISA analysis of PABL-209 was performed by coating with Influenza A H2N2 (A/Japan/305/1957) Hemagglutinin / HA Protein (His Tag). Then blocked with BSA and incubated with PABL-209. The HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 4 Anti-Influenza A Virus HA Recombinant Antibody (PABL-209) in DB

Dot Blot analysis of PABL-209 was performed by coating with Influenza A H2N2 (A/Japan/305/1957) Hemagglutinin / HA Protein (His Tag).

PABL-209 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000)