Recombinant Mouse Anti-Kdo Antibody (S73-2) (CAT#: PABW-156)
Recombinant Mouse Antibody (S73-2) is capable of binding to Kdo, expressed in Chinese Hamster Ovary cells (CHO). The antibody S73-2 displayed a broad specificity, binding with the same affinity to its immunizing antigen Kdo(2→8)[Kdo(2→4)]Kdo and all structures containing the 2→4-2→4-linked trisaccharide or the branched Kdo tetrasaccharide. The antibody cross-reacted with the family-specific Kdo(2→8)Kdo(2→4)Kdo linked trisaccharide and with both, the 2→4 and 2→8-linked disaccharides indicating that the antibody may not discriminate between C. psittaci and the other chlamydiae species.
Specific Inquiry
SPR
Figure 1 Surface plasmon resonance analysis of S73-2 Fab binding to different Kdo antigens
Sensorgram overlays for the binding of 10, 20, 40, 80, 160, 320, 640 and 1,280 nM S73-2 Fab to 160 RUs of immobilized Kdo(2→8)Kdo(2→4)Kdo-BSA. The open black circles are the data points and the red curves the fitting of the data to a 1:1 interaction model.
Brooks, C. L., Müller-Loennies, S., Borisova, S. N., Brade, L., Kosma, P., Hirama, T., ... & Evans, S. V. (2010). Antibodies raised against chlamydial lipopolysaccharide antigens reveal convergence in germline gene usage and differential epitope recognition. Biochemistry, 49(3), 570-581.
SPR
Figure 2 Surface plasmon resonance analysis of S73-2 Fab binding to different Kdo antigens
Sensorgram overlays for the binding of 1, 2, 3, 5, 7 and 10 nM S73-2 Fab to 2,460 RUs of immobilized Kdo(2→8)Kdo(2→4)Kdo-BSA.
Brooks, C. L., Müller-Loennies, S., Borisova, S. N., Brade, L., Kosma, P., Hirama, T., ... & Evans, S. V. (2010). Antibodies raised against chlamydial lipopolysaccharide antigens reveal convergence in germline gene usage and differential epitope recognition. Biochemistry, 49(3), 570-581.
SPR
Figure 3 Surface plasmon resonance analysis of S73-2 Fab binding to different Kdo antigens
Standard curves for S73-2 Fab concentration assay based on the steady state binding data from C- the inset shows linear fitting (red lines) of the initial data points (open black circles).
Brooks, C. L., Müller-Loennies, S., Borisova, S. N., Brade, L., Kosma, P., Hirama, T., ... & Evans, S. V. (2010). Antibodies raised against chlamydial lipopolysaccharide antigens reveal convergence in germline gene usage and differential epitope recognition. Biochemistry, 49(3), 570-581.
SPR
Figure 4 Surface plasmon resonance analysis of S73-2 Fab binding to different Kdo antigens
Fitting to a solution affinity model of data for the inhibition of S73-2 Fab binding to 1,650 or 2,460 RUs of immobilized Kdo(2→8)Kdo(2→4)Kdo-BSA by various saccharides (○)Kdo; Kdo(2→8)Kdo; (◆) Kdo(2→8)Kdo(▼)Kdo(2→4)Kdo; (□)Kdo(2→8)Kdo(2→4)Kdo; (■)Kdo(2→4)Kdo(2→4)Kdo
Brooks, C. L., Müller-Loennies, S., Borisova, S. N., Brade, L., Kosma, P., Hirama, T., ... & Evans, S. V. (2010). Antibodies raised against chlamydial lipopolysaccharide antigens reveal convergence in germline gene usage and differential epitope recognition. Biochemistry, 49(3), 570-581.
Specifications
- Immunogen
- 3-deoxy-α-D-manno-oct-2-ulosonic acid
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against synthetic Kdo (3-deoxy-α-D-manno-oct-2-ulosonic acid)
- Clone
- S73-2
- Applications
- Can be useful in applications such as: Enzyme-linked Immunosorbent Assay
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Target
- Alternative Names
- Kdo; 3-Deoxy-D-manno-oct-2-ulosonic acid; 2-Oxo-3-deoxy-D-mannooctonic acid; 2-Keto-3-Deoxy-D-manno-octonate; 2-Keto-3-deoxy-D-mannooctanoic acid; 3-Deoxy-D-manno-octulosonic acid; 3-Deoxy-D-manno-2-octulosonic acid
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-598-S(P) | Recombinant Anti-KDO Antibody scFv Fragment | Neut, WB, FuncS | scFv |
| MHH-598-S(P) | Recombinant Human Anti-KDO Antibody scFv Fragment | FC, ELISA, FuncS | scFv |
| PFBW-150 | Recombinant Mouse Anti-Kdo Antibody Fab Fragment (S67-27) | ELISA | Fab |
| PFBW-155 | Mouse Anti-KDO Recombinant Antibody (clone S54-10); scFv Fragment | ELISA | Mouse Fab |
| PFBW-156 | Recombinant Mouse Anti-Kdo Antibody Fab Fragment (S73-2) | ELISA | Fab |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-254 | Recombinant Mouse Anti-KDO Antibody (S25-2 ) | WB, IHC, FuncS | IgG |
| PABL-626 | Mouse Anti-KDO Recombinant Antibody (clone S25-39) | ELISA | Mouse IgG |
| PABL-627 | Mouse Anti-KDO Recombinant Antibody (clone S64-4) | ELISA | Mouse IgG |
| PABL-628 | Mouse Anti-KDO Recombinant Antibody (clone S67-27) | ELISA | Mouse IgG |
| PABL-629 | Mouse Anti-KDO Recombinant Antibody (clone S73-2) | ELISA | Mouse IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-254 | Recombinant Mouse Anti-KDO Antibody Fab Fragment (S25-2 ) | WB, IHC, FuncS | Fab |
| PFBL-621 | Mouse Anti-KDO Recombinant Antibody (clone S25-39); Fab Fragment | ELISA | Mouse Fab |
| PFBL-622 | Mouse Anti-KDO Recombinant Antibody (clone S64-4); Fab Fragment | ELISA | Mouse Fab |
| PFBL-623 | Mouse Anti-KDO Recombinant Antibody (clone S67-27); Fab Fragment | ELISA | Mouse Fab |
| PFBL-624 | Mouse Anti-KDO Recombinant Antibody (clone S73-2); Fab Fragment | ELISA | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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