Recombinant Mouse Anti-Progesterone Antibody Fab Fragment (DB3)
CAT#: PABX-159-F (E)
Anti-Progesterone antibody is a Mouse of Fab class that binds to an Progesterone. The monoclonal anti-progesterone antibody binds progesterone with nanomolar affinity (Ka approximately 10 (9) M-1).
Published Data
-1.png)
Figure 1 Structure of the DB4 combining site.
(A) Crystal structure of the complex between DB3 and progesterone (Arevalo et al.1994a) showing some of the contact residues. Light and the heavy chains are in blue and purple, respectively; steroid oxygen atoms in red; steroid A-ring asterisked. (B) Mode
He, M., Hamon, M., Liu, H., Corper, A. L., & Taussig, M. J. (2006). Effects of mutation at the D‐JH junction on affinity, specificity, and idiotypy of anti‐progesterone antibody DB3. Protein science, 15(9), 2141-2149.
-2.png)
Figure 2 Western blot of wild type and mutants of DB4 VH/K.
Samples were run on 11% SDS-PAGE and electrotransferred to an Immobilon-P membrane (PVDF); the blot was probed with HRP-coupled sheep antimouse k.
He, M., Hamon, M., Liu, H., Corper, A. L., & Taussig, M. J. (2006). Effects of mutation at the D‐JH junction on affinity, specificity, and idiotypy of anti‐progesterone antibody DB3. Protein science, 15(9), 2141-2149.
-3.png)
Figure 3 Binding specificity of wild-type DB3 VH/K and the TrpH101Arg mutant for progesterone derivatives.
Binding to progesterone-BSA of wild-type DB3 VH/K (A) and the TrpH100Arg mutant (B) was carried out in the presence of free progesterone-3-CMO (C3-CMO), progesterone-6-HMS (C6-HMS), progesterone-11a-HMS (C11a-HMS),or progesterone-21-HMS (C22-HMS). ResultsȔ
He, M., Hamon, M., Liu, H., Corper, A. L., & Taussig, M. J. (2006). Effects of mutation at the D‐JH junction on affinity, specificity, and idiotypy of anti‐progesterone antibody DB3. Protein science, 15(9), 2141-2150.
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Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Fab
- Specificity
- Tested positive against native Progesterone
- Clone
- DB3
- Applications
- Neut (assay dependent)
FuncS (assay dependent)
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- Assay-depended
Target
- Alternative Names
- Progesterone; P4; pregn-4-ene-3,20-dione
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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See other products for "Clone DB3"
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-311 | Human Anti-P4 Recombinant Antibody (clone 1E9-DB3) | WB, ELISA, FuncS | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-311 | Human Anti-P4 Recombinant Antibody (clone 1E9-DB3); scFv Fragment | WB, ELISA, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-311 | Human Anti-P4 Recombinant Antibody (clone 1E9-DB3); Fab Fragment | WB, ELISA, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-159 | Recombinant Mouse Anti-Progesterone Antibody (DB3) | Neut, FuncS | IgG |
| PABX-159-S (P) | Recombinant Mouse Anti-Progesterone Antibody scFv Fragment (DB3) | Neut, FuncS | scFv |
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