Rabbit Anti-ABCD1 Recombinant Antibody (clone R64-2S-2)

CAT#: VS3-FY2187

Clone R64-2S-2 is a recombinant monoclonal antibody that binds to human ABCD1 protein, a calculated weight of 83 kDa. It cross-reacts with mouse, rat. For detection of human ABCD1 using flow cytometry, please refer to clone R64-2S-2.

Specifications

  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse and Rat ABCD1
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R64-2S-2
  • Applications
  • Western Blot, Immunofluorescence, Flow Cytometry
  • Conjugate
  • Unconjugated
  • MW
  • The calculated weight of the target protein is approximately 83kDa, while the observed molecular weight stands at 83kDa.
  • Related Disease
  • Adrenoleukodystrophy and Hypoadrenocorticism, Familial

Product Property

  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS (pH 7.4), 150 mM NaCl, 50% glycerol
  • Preservative
  • 0.02% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunofluorescence: 1/50-1/200
    Flow Cytometry: 1/50

Target

  • Alternative Names
  • ATP Binding Cassette Subfamily D Member 1; ATP-Binding Cassette, Sub-Family D (ALD), Member 1; Adrenoleukodystrophy Protein; ALDP; ALD; ATP-Binding Cassette Sub-Family D Member 1; ABC42; AMN
  • Sequence Similarities
  • Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.
  • Cellular Localization
  • Endoplasmic reticulum, Lysosome, Membrane, Mitochondrion, Peroxisome
  • Post Translation Modifications
  • Tyrosine-phosphorylated. (P33897-ABCD1_HUMAN)
    Glycosylation at Asn214 (NX_P33897 [NX_P33897-1])
    Ubiquitination at Lys708 (NX_P33897 [NX_P33897-1])
    Modification sites at PhosphoSitePlus (P33897)
    Glycosylation from GlyGen (P33897) 1 site
  • Function
  • Coupled to the ATP-dependent transporter activity has also a fatty acyl-CoA thioesterase activity (ACOT) and hydrolyzes VLCFA-CoA into VLCFA prior their ATP-dependent transport into peroxisomes, the ACOT activity is essential during this transport process.
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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

Secondary Antibody

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VS-0325-FY180 Human Anti-ABCD1 scFv-Fc Chimera (VS-0325-FY180) IA Human IgG1, scFv-Fc
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