Rabbit Anti-AIMP1 Recombinant Antibody (clone R01-5A9)
CAT#: VS3-FY1707
The R01-5A9 recombinant monoclonal antibody specifically targets human AIMP1. It cross-reacts with rat. The EMAP II antibody is recommended for detection of target protein by western blot analysis of EMAP II in C6 lysates.
Tested Data
Gene Expression
WB
Figure 1 Anti-AIMP1 Antibody (VS3-FY1707) in WB.
The EMAP II expression in C6 lysates were detected using the anti-EMAP II by western blot assay.
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17618/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human hippocampus
Immunohistochemical staining of human hippocampus shows distinct cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18476/ihc_selected.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17618/44484_A_8_8.jpg
Normal Tissue
Figure 4 Cerebral cortex
Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17618/44484_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17618/44484_A_8_3.jpg
Normal Tissue
Figure 6 Pancreas
Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Pancreatic endocrine cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17618/44484_A_1_3.jpg
Normal Tissue
Figure 7 Testis
Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17618/44484_A_5_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000164022-AIMP1
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Specifications
- Immunogen
- Synthetic peptide of human EMAP II
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat AIMP1
- Species Reactivity
- Human, Rat
- Clone
- R01-5A9
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 34 kDa, while the observed molecular weight stands at 34 kDa.
- Related Disease
- Leukodystrophy, Hypomyelinating, 3 and Autosomal Recessive Non-Syndromic Intellectual Disability
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Aminoacyl TRNA Synthetase Complex Interacting Multifunctional Protein 1; Small Inducible Cytokine Subfamily E, Member 1 (Endothelial Monocyte-Activating); Multisynthase Complex Auxiliary Component P43; ARS-Interacting Multifunctional Protein 1; SCYE1; EMAP2; Aminoacyl TRNA Synthase Complex-Interacting Multifunctional Protein 1; Multisynthetase Complex Auxiliary Component P43
- Gene ID
- 9255
- UniProt ID
- Q12904
- Cellular Localization
- Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Nucleus, Secreted
- Post Translation Modifications
- Cleaved by caspase-7 in response to apoptosis to produce EMAP-II.
- Protein Refseq
- XP_016864324.1; XP_047272366.1; XP_054207221.1; NP_001135887.1; NP_001135888.2
- Function
- Non-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase.
Binds tRNA. Possesses inflammatory cytokine activity.
Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation (By similarity).
Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "AIMP1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0645CT | Mouse Anti-AIMP1 Recombinant Antibody (clone 1C11) | ELISA, WB | Mouse IgG2a |
| HPAB-0344-YJ | Rat Anti-AIMP1 Recombinant Antibody (HPAB-0344-YJ) | ELISA, WB, Inhib, FuncS | Rat IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0526MZ | Chicken Anti-SCYE1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0344-YJ-S(P) | Rat Anti-AIMP1 Recombinant Antibody; scFv Fragment (HPAB-0344-YJ-S(P)) | ELISA, IF, Neut | Rat scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0344-YJ-F(E) | Rat Anti-AIMP1 Recombinant Antibody; Fab Fragment (HPAB-0344-YJ-F(E)) | ELISA, IF, Neut | Rat Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1225 | AbPlus™ Anti-Aimp1 Magnetic Beads (VS-0724-YC1225) | IP, Protein Purification | |
| VS-0724-YC1226 | AbPlus™ Anti-AIMP1 Magnetic Beads (VS-0724-YC1226) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY70 | Anti-AIMP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY248 | Anti-Mouse AIMP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY247 | Anti-Human AIMP1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC10 | Recombinant Anti-AIMP1 Eliminating Antibody, pH-Sensitive (VS-0625-YC10) | Antigen-Sweeping In Vivo. | Rat IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC213 | SmartAb™ Recombinant Anti-AIMP1 pH-dependent Antibody (VS-0825-YC213) | ELISA, WB, Inhib | Rat IgG |
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