Human Anti-BoNT Recombinant Antibody (clone 4E17) (CAT#: VS-0623-WK83)
The antibody clone 4E17 was produced by immunizing with purified BoNT. It can be used for detection of Botulinum neurotoxins in ELSIA and FC assays.
Specific Inquiry
FC
Figure 1 Binding of 1B18 and 4E17.1 mAbs to wild-type BoNT/B HN and the BoNT/B HN E747A mutant by flow cytometry.
1B11 binds to both the wild-type and mutant HN, whereas neither 1B18 nor 4E17.1 bind to the HN E747A mutant. The results indicate that the fine epitope of both 1B18 and 4E17.1 is located around HN amino acid E747.
Neut
Figure 2 Potency of in vivo BoNT neutralization by mAbs 2B18.1 and 4E17.1.
For both BoNT/B and E, 25 µg of 2B18.1 and 4E17.1 completely protected mice challenged with 200 mouse lethal doses 50% (LD50) of BoNT/B or E, respectively.
Specifications
- Immunogen
- Purified BoNT
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Botulinum neurotoxins
- Species Reactivity
- Clostridium botulinum
- Clone
- 4E17
- Applications
- ELSIA, FC, Neut
- Conjugate
- Unconjugated
- Related Disease
- Botulism
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No Preservative
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Neutralization: 1 µg/mL-100 µg/mL
Flow cytometry: 1.6 pM-1 µM
Target
- Alternative Names
- Botulinum neurotoxin (BoNT)
- Gene ID
- 5185061
- UniProt ID
- P10845
- Long Name
- Botulinum neurotoxin
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Mouse Antibody Products
ScFv Antibody Products
Fab Antibody Products
IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-140CT | Anti-C. botulinum neurotoxin type B Recombinant Antibody (MRO-140CT) | ELISA, WB, Neut | |
| MRO-140CT-S(P) | Anti-C. botulinum neurotoxin type B Recombinant Antibody scFv Fragment (MRO-140CT-S(P)) | ELISA, Neut, WB | |
| MRO-140CT-F(E) | Anti-C. botulinum neurotoxin type B Recombinant Antibody Fab Fragment (MRO-140CT-F(E)) | ELISA, Neut, WB |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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