Mouse Anti-CD20 Recombinant Antibody (clone scFv-7) (CAT#: FAMAB-0462WJ)
This product is a mouse monoclonal antibody that is specific for canine CD20. This antibody can be used in a variety of applications, such as ELISA, WB.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Figure 1 The indicated scFv (3 or 7) was cloned into pCDNA3.1 containing a leader sequence (amino acids MGGS) for targeted secretion into the media of tissue cultured CHO cells.
(i) Binding was measured on left panel after dilution of the supernatants (1:40 or 1:80) to optimize binding against His-tagged CD20 protein; (ii) right panel the optimized supernatants of scFv-3 and scFv-7 were assayed against biotinylated CD20 peptide as indicated in the materials and methods. Antibody scFv binding to antigen was detected using peroxidase conjugated protein A and resolved with TMB-based ELISA at an OD of 450nm. Controls included media only or PBS.
Jain, S., Aresu, L., Comazzi, S., Shi, J., Worrall, E., Clayton, J., ... & Hupp, T. R. (2016). The development of a recombinant scFv monoclonal antibody targeting canine CD20 for use in comparative medicine. PloS one, 11(2), e0148366.
Figure 2 Bioactivity of affinity purified scFv-3 and scFv-7 from bacteria.
The normalized affinity-purified scFv fractions were assayed for binding to the indicated canine CD20 peptides (peptide 32 and peptide 38), where the core epitope resides (in gold shade). scFv-3:CPG2 was more active than scFv-7:CPG2 on both peptide 32 and peptide 38. The enhanced binding of scFv-3:CPG2 as a bioconjugate is consistent with its enhanced binding when secreted as a single chain from CHO cells and when fused to M13 gIII protein.
Jain, S., Aresu, L., Comazzi, S., Shi, J., Worrall, E., Clayton, J., ... & Hupp, T. R. (2016). The development of a recombinant scFv monoclonal antibody targeting canine CD20 for use in comparative medicine. PloS one, 11(2), e0148366.
Specifications
- Immunogen
- A peptide derived from canine CD20 NCDPANPSEKNSLSIQYCGS
- Host Species
- Mouse
- Derivation
- Hybridoma
- Type
- Mouse IgG
- Specificity
- Canine CD20
- Species Reactivity
- Canine
- Clone
- scFv-7
- Applications
- ELISA, WB
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- B1; S7; Bp35; CD20; CVID5; MS4A1; LEU-16
- Gene ID
- 931
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibody Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-1621CQ | Human Anti-CD20 Recombinant Antibody (clone 18B12) | Depletion, FuncS, WB, FC, IF | Human IgG1 |
| VS7-HM449 | Mouse Anti-CD20 Recombinant Antibody (clone 3E9D3C1G3) | IHC, ICC | Mouse IgG |
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