Mouse Anti-CD22 Recombinant Antibody (clone m5/44) (CAT#: FAMAB-0046YC)

Figure 1 Mouse Anti-CD22 Antibody (clone m5/44) in Neut.

Competitive inhibition of the binding of murine antiCD22 mAb, m5/44, to Ramos BCL by its humanized versions assembled by combinations of four distinct grafts of heavy chain (gHa-d) and two distinct grafts of light chain (gLa, gLb). Increasing concentrations of the humanized mAb were mixed with the fixed concentration of m5/44 and then allowed to bind to Ramos BCL. Binding of the murine mAb, m5/44, was monitored by indirect immunofluorescence analysis using flow cytometry. The chimeric version of m5/44 (cL cH) was also used in this evaluation as a positive control

DiJoseph, J. F., Popplewell, A., Tickle, S., Ladyman, H., Lawson, A., Kunz, A., ... & Damle, N. K. (2005). Antibody-targeted chemotherapy of B-cell lymphoma using calicheamicin conjugated to murine or humanized antibody against CD22. Cancer Immunology, Immunotherapy, 54(1), 11-24.

Figure 1 Recombinant Mouse Anti-CD22 Antibody (FAMAB-0046YC) in SDS-PAGE

SDS-PAGE analysis of FAMAB-0046YC in non-reduced and β-mercaptoethanol-reduced conditions.

Figure 2 Recombinant Mouse Anti-CD22 Antibody (FAMAB-0046YC) in HPLC

The purity of FAMAB-0046YC was greater than 95% as determined by SEC-HPLC.