Rabbit Anti-CD3E Recombinant Antibody (clone N26-R)
CAT#: ZG-0312U
Rabbit anti-CD3E antibody (clone N26-R) displayed high specificity against the CD3E protein of Human.
Tested Data
Gene Expression
IHC-P
Figure 1 Rabbit Anti-CD3E Antibody (ZG-0312U) in IHC-P
CD3 positivity in T-lymphocytes of the normal lymph node, stained with anti-CD3 antibody. Formalin fixed, paraffin embedded human tissue (4 μm section) stained.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line JURKAT
Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane & endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40957/1970_E5_1_selected.jpg
Normal Tissue
Figure 2 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72864/157655_A_9_8.jpg
RNA Expression
Figure 3 RNA cell line category: Group enriched (JURKAT, MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198851-CD3E
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Specifications
- Immunogen
- Peptide derived from cytoplasmic, C-terminal region of human CD3-epsilon chain
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CD3E
- Species Reactivity
- Human
- Clone
- N26-R
- Applications
- IHC-P
- Conjugate
- Unconjugated
- Related Disease
- Immunodeficiency 18 and T-B+ Severe Combined Immunodeficiency Due To Cd3delta/Cd3epsilon/Cd3zeta.
Product Property
- Purification
- Affinity Purified
- Buffer
- 20 mM Tris-HCl, pH 8.0, 20 mg/ml BSA and 0.05% sodium azide
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Immunohistochemistry-Paraffin. Specific conditions should be optimized by the end user.
IHC-P: 1:100-1:200
Target
- Alternative Names
- T3E; TCRE; IMD18; T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain
- Gene ID
- 916
- UniProt ID
- P07766
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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