Anti-CFL1 Immunohistochemistry Kit
CAT#: VS-0325-XY433
Our CFL1 IHC Kit comes with all required reagents. It is designed to be user - friendly, facilitating the IHC procedure. Using this kit, researchers can precisely detect CFL1 protein expression, which is important for understanding actin - cytoskeleton dynamics and cell motility. This kit can be applied to paraffin sections and frozen sections.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53761/832_H1_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human colon
Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53761/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human colon
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/33687/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human testis
Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells of seminiferus ducts.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37077/ihc_selected.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53761/134168_A_8_3.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53761/134168_A_6_6.jpg
Normal Tissue
Figure 7 Fallopian tube
Ciliated cells (cilia axoneme)
Staining:Medium
Intensity: Moderate
Ciliated cells (ciliary rootlets)
Staining:Medium
Intensity: Moderate
Non-ciliated cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53761/134168_A_7_7.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53761/134168_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000172757-CFL1
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Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Human, Mouse, Rat
- Target
- CFL1
- Primary Antibody
- Mouse Anti-CFL1 Monoclonal Antibody
- Secondary Antibody
- Goat anti-Mouse Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
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Datasheet
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COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| MOB-196-S(P) | Recombinant Anti-human CFL1 Antibody scFv Fragment | IF, Neut, FuncS | scFv |
| MHH-196-S(P) | Recombinant Human Anti-human CFL1 Antibody scFv Fragment | ELISA, IP, FuncS | scFv |
| CAT | Product Name | Application | Type |
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| MOR-0675 | Hi-Affi™ Rabbit Anti-CFL1 Recombinant Antibody (clone DS675AB) | WB, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| VS13-YC215 | CytoStream™ Rabbit Anti-CFL1 Recombinant Antibody (VS13-YC215) | WB, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1399 | Anti-Mouse CFL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1398 | Anti-Human CFL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1400 | Anti-Monkey CFL1 Immunohistochemistry Kit | IHC |
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