Recombinant Mouse anti-Human CFL1 Monoclonal antibody (EML010)
CAT#: MOB-2448CT
Recombinant Mouse Monoclonall antibody to Human CFL1, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53761/832_H1_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human colon
Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53761/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human colon
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/33687/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human testis
Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells of seminiferus ducts.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37077/ihc_selected.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53761/134168_A_8_3.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53761/134168_A_6_6.jpg
Normal Tissue
Figure 7 Fallopian tube
Ciliated cells (cilia axoneme)
Staining:Medium
Intensity: Moderate
Ciliated cells (ciliary rootlets)
Staining:Medium
Intensity: Moderate
Non-ciliated cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53761/134168_A_7_7.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53761/134168_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000172757-CFL1
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Specifications
- Immunogen
- Recombinant full length protein, corresponding to amino acids 1-167 of Human Cofilin
- Host Species
- Mouse
- Specificity
- Widely distributed in various tissues.
- Species Reactivity
- Human
- Clone
- EML010
- Applications
- Used for immunoassay techniques such as: Western Blot; Immunohistochemistry paraffin embedded sections; Immunocytochemistry/Immunofluorescence
- Conjugate
- Unconjugated
Product Property
- Purity
- Protein G purified
- Format
- Liquid
- Buffer
- Preservative: None PBS, pH 7.2
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| MOB-196 | Recombinant Anti-human CFL1 Antibody | WB, Neut, FuncS | IgG |
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| CAT | Product Name | Application | Type |
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| MOB-196-F(E) | Recombinant Anti-human CFL1 Antibody Fab Fragment | RIA, ELISA, FuncS | Fab |
| MHH-196-F(E) | Recombinant Human Anti-human CFL1 Antibody Fab Fragment | RIA, WB, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-196-S(P) | Recombinant Anti-human CFL1 Antibody scFv Fragment | IF, Neut, FuncS | scFv |
| MHH-196-S(P) | Recombinant Human Anti-human CFL1 Antibody scFv Fragment | ELISA, IP, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0675 | Hi-Affi™ Rabbit Anti-CFL1 Recombinant Antibody (clone DS675AB) | WB, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY433 | Anti-CFL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1399 | Anti-Mouse CFL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1398 | Anti-Human CFL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1400 | Anti-Monkey CFL1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC215 | CytoStream™ Rabbit Anti-CFL1 Recombinant Antibody (VS13-YC215) | WB, ICC, IF, FC | Rabbit IgG |
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