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  • Recombinant Mouse Anti-CFL1 Antibody (VS-0923-FY42)

Recombinant Mouse Anti-CFL1 Antibody (VS-0923-FY42) (CAT#: VS-0923-FY42)

The VS-0923-FY42 is a murine IgG2b antibody that binds to CFL1. This antibody was originally generated in mice immuned with the full length of human CFL1.


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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53761/832_H1_2_selected.jpg
Figure 2 IHC staining of human colon
Figure 2 IHC staining of human colon
(Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53761/ihc_selected.jpg
Figure 3 IHC staining of human colon
Figure 3 IHC staining of human colon
(Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/33687/ihc_selected.jpg
Figure 4 IHC staining of human testis
Figure 4 IHC staining of human testis
(Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells of seminiferus ducts.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37077/ihc_selected.jpg
Normal Tissue
Figure 5 Colon
Figure 5 Colon
(Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53761/134168_A_8_3.jpg
Figure 6 Testis
Figure 6 Testis
(Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53761/134168_A_6_6.jpg
Figure 7 Fallopian tube
Figure 7 Fallopian tube
(Ciliated cells (cilia axoneme)
Staining:Medium
Intensity: Moderate
Ciliated cells (ciliary rootlets)
Staining:Medium
Intensity: Moderate
Non-ciliated cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53761/134168_A_7_7.jpg
Figure 8 Lymph node
Figure 8 Lymph node
(Germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53761/134168_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000172757-CFL1

Specifications

  • Immunogen
  • The full-length CFL1 protein
  • Host Species
  • Mouse
  • Type
  • Mouse IgG2b
  • Specificity
  • Human CFL1
  • Species Reactivity
  • Human
  • Applications
  • ELISA, Western Blot
  • Conjugate
  • Unconjugated

Product Property

  • Format
  • Liquid
  • Buffer
  • Citrate Tris HCl buffer
  • Preservative
  • 0.02% Proclin 300
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot (1/1000)
    Specific conditions should be optimized by the end user.

Target

  • Alternative Names
  • Cofilin 1; Cofilin, Non-Muscle Isoform; Cofilin 1 (Non-Muscle); 18 KDa Phosphoprotein; P18; CFL

Recommended Products

Isotype Control

Secondary Antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "CFL1"

Single-domain Antibody

CAT Product Name Application Type
NAB-385-VHH Recombinant Anti-human CFL1 VHH Single Domain Antibody FUNC, FA, IP, ChiP Llama VHH

Recombinant Antibody

CAT Product Name Application Type
MOB-196 Recombinant Anti-human CFL1 Antibody WB, Neut, FuncS IgG
MHH-196 Recombinant Human Anti-human CFL1 Antibody ELISA, WB, FuncS IgG
MOB-2448CT Recombinant Mouse anti-Human CFL1 Monoclonal antibody (EML010) WB, IHC-P, ICC/IF
VS3-FY372 Recombinant Rabbit Anti-CFL1 Antibody (clone R06-2C0) WB, IHC-F, IHC-P, ICC, IF Rabbit IgG

Fab Fragment Antibody

CAT Product Name Application Type
MOB-196-F(E) Recombinant Anti-human CFL1 Antibody Fab Fragment RIA, ELISA, FuncS Fab
MHH-196-F(E) Recombinant Human Anti-human CFL1 Antibody Fab Fragment RIA, WB, FuncS Fab

scFv Fragment Antibody

CAT Product Name Application Type
MOB-196-S(P) Recombinant Anti-human CFL1 Antibody scFv Fragment IF, Neut, FuncS scFv
MHH-196-S(P) Recombinant Human Anti-human CFL1 Antibody scFv Fragment ELISA, IP, FuncS scFv

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0675 Hi-Affi™ Rabbit Anti-CFL1 Recombinant Antibody (clone DS675AB) WB, ICC Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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