Recombinant Mouse Anti-CFL1 Antibody (VS-0923-FY42)
CAT#: VS-0923-FY42
The VS-0923-FY42 is a murine IgG2b antibody that binds to CFL1. This antibody was originally generated in mice immuned with the full length of human CFL1.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53761/832_H1_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human colon
Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53761/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human colon
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/33687/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human testis
Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells of seminiferus ducts.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37077/ihc_selected.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53761/134168_A_8_3.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53761/134168_A_6_6.jpg
Normal Tissue
Figure 7 Fallopian tube
Ciliated cells (cilia axoneme)
Staining:Medium
Intensity: Moderate
Ciliated cells (ciliary rootlets)
Staining:Medium
Intensity: Moderate
Non-ciliated cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53761/134168_A_7_7.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53761/134168_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000172757-CFL1
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Specifications
- Immunogen
- The full-length CFL1 protein
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human CFL1
- Species Reactivity
- Human
- Applications
- ELISA, Western Blot
- Conjugate
- Unconjugated
Product Property
- Format
- Liquid
- Buffer
- Citrate Tris HCl buffer
- Preservative
- 0.02% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot (1/1000)
Specific conditions should be optimized by the end user.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CFL1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-385-VHH | Recombinant Anti-human CFL1 VHH Single Domain Antibody | FUNC, FA, IP, ChiP | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-196 | Recombinant Anti-human CFL1 Antibody | WB, Neut, FuncS | IgG |
| MHH-196 | Recombinant Human Anti-human CFL1 Antibody | ELISA, WB, FuncS | IgG |
| MOB-2448CT | Recombinant Mouse anti-Human CFL1 Monoclonal antibody (EML010) | WB, IHC-P, ICC, IF | |
| VS3-FY372 | Recombinant Rabbit Anti-CFL1 Antibody (clone R06-2C0) | WB, IHC-F, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-196-F(E) | Recombinant Anti-human CFL1 Antibody Fab Fragment | RIA, ELISA, FuncS | Fab |
| MHH-196-F(E) | Recombinant Human Anti-human CFL1 Antibody Fab Fragment | RIA, WB, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-196-S(P) | Recombinant Anti-human CFL1 Antibody scFv Fragment | IF, Neut, FuncS | scFv |
| MHH-196-S(P) | Recombinant Human Anti-human CFL1 Antibody scFv Fragment | ELISA, IP, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0675 | Hi-Affi™ Rabbit Anti-CFL1 Recombinant Antibody (clone DS675AB) | WB, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY433 | Anti-CFL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1399 | Anti-Mouse CFL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1398 | Anti-Human CFL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1400 | Anti-Monkey CFL1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC215 | CytoStream™ Rabbit Anti-CFL1 Recombinant Antibody (VS13-YC215) | WB, ICC, IF, FC | Rabbit IgG |
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