Recombinant Rabbit Anti-CFL1 Antibody (clone R06-2C0)
CAT#: VS3-FY372
This product is a recombinant rabbit antibody that recognizes human CFL1 protein. It can detect a band of approximately 19 kDa (observedd molecular weight: 19 kDa).
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53761/832_H1_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human colon
Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53761/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human colon
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/33687/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human testis
Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells of seminiferus ducts.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37077/ihc_selected.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53761/134168_A_8_3.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53761/134168_A_6_6.jpg
Normal Tissue
Figure 7 Fallopian tube
Ciliated cells (cilia axoneme)
Staining:Medium
Intensity: Moderate
Ciliated cells (ciliary rootlets)
Staining:Medium
Intensity: Moderate
Non-ciliated cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53761/134168_A_7_7.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53761/134168_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000172757-CFL1
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IHC
Figure 1. Recombinant Rabbit Anti-CFL1 Antibody (clone R06-2C0) in IHC.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Cofilin antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- A synthetic peptide of human Cofilin
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CFL1
- Species Reactivity
- Human
- Clone
- R06-2C0
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 19 kDa; Observed MW: 19 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "CFL1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-385-VHH | Recombinant Anti-human CFL1 VHH Single Domain Antibody | FUNC, FA, IP, ChiP | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-196 | Recombinant Anti-human CFL1 Antibody | WB, Neut, FuncS | IgG |
| MHH-196 | Recombinant Human Anti-human CFL1 Antibody | ELISA, WB, FuncS | IgG |
| MOB-2448CT | Recombinant Mouse anti-Human CFL1 Monoclonal antibody (EML010) | WB, IHC-P, ICC, IF | |
| VS-0923-FY42 | Recombinant Mouse Anti-CFL1 Antibody (VS-0923-FY42) | ELISA, WB | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-196-F(E) | Recombinant Anti-human CFL1 Antibody Fab Fragment | RIA, ELISA, FuncS | Fab |
| MHH-196-F(E) | Recombinant Human Anti-human CFL1 Antibody Fab Fragment | RIA, WB, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-196-S(P) | Recombinant Anti-human CFL1 Antibody scFv Fragment | IF, Neut, FuncS | scFv |
| MHH-196-S(P) | Recombinant Human Anti-human CFL1 Antibody scFv Fragment | ELISA, IP, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0675 | Hi-Affi™ Rabbit Anti-CFL1 Recombinant Antibody (clone DS675AB) | WB, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY433 | Anti-CFL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1399 | Anti-Mouse CFL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1398 | Anti-Human CFL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1400 | Anti-Monkey CFL1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC215 | CytoStream™ Rabbit Anti-CFL1 Recombinant Antibody (VS13-YC215) | WB, ICC, IF, FC | Rabbit IgG |
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