Mouse Anti-FZR1 Recombinant Antibody (VS3-FY2714) (CAT#: VS3-FY2714)
This anti-FZR1 product is a mouse recombinant monoclonal antibody that has been carefully developed to target FZR. The calculated molecular weight of the antigen is 55.2 kDa. It shows capability of cross-reacting with human and mouse. This monoclonal antibody is potential for conducting research on Developmental and Epileptic Encephalopathy 109 and Non-Specific Early-Onset Epileptic Encephalopathy. This monoclonal antibody can be used in Western Blot.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43536/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43536/if_selected.jpg
Figure 2 IHC staining of human liver
(Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43536/ihc_selected.jpg
(Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43536/ihc_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43536/98029_B_8_5.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43536/98029_B_8_5.jpg
Figure 4 Colon
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43536/98029_A_9_3.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43536/98029_A_9_3.jpg
Figure 5 Liver
(Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43536/98029_A_8_4.jpg
(Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43536/98029_A_8_4.jpg
Figure 6 Kidney
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43536/98029_A_9_5.jpg
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43536/98029_A_9_5.jpg
Figure 7 Testis
(Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43536/98029_A_6_6.jpg
(Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43536/98029_A_6_6.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105325-FZR1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105325-FZR1
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Fizzy and cell division cycle 20 related 1 of human and mouse origins
- Species Reactivity
- Human, Mouse
- Applications
- WB
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 55.2 kDa.
- Related Disease
- Developmental And Epileptic Encephalopathy 109 and Non-Specific Early-Onset Epileptic Encephalopathy
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Target
- Alternative Names
- Fizzy And Cell Division Cycle 20 Related 1; Fizzy/Cell Division Cycle 20 Related 1; CDC20-Like Protein 1; Cdh1/Hct1 Homolog; CDC20 Homolog 1; HCDH1; CDH1; FZR; Fizzy/Cell Division Cycle 20 Related 1 (Drosophila)
- Gene ID
- 51343
- UniProt ID
- Q9UM11
- Sequence Similarities
- Belongs to the WD repeat CDC20/Fizzy family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Acetylated.
Deacetylated by SIRT2 at Lys-69 and Lys-159; deacetylation enhances the interaction of FZR1 with CDC27, leading to activation of anaphase promoting complex/cyclosome (APC/C).
Following DNA damage, it is dephosphorylated by CDC14B in G2 phase, leading to its reassociation with the APC/C, and allowing an efficient G2 DNA damage checkpoint.
Phosphorylated by MAK.
Ubiquitinated by the SCF(CCNF) E3 ubiquitin-protein ligase complex; leading to its degradation by the proteasome.
Ubiquitination at Lys135 and Lys263
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 4 sites, 1 O-linked glycan (4 sites)
- Protein Refseq
- XP_005259630.1; XP_016882352.1; XP_047294864.1; XP_047294865.1; XP_054177152.1
- Function
- Acts as an adapter for APC/C to target the DNA-end resection factor RBBP8/CtIP for ubiquitination and subsequent proteasomal degradation.
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Please select product type
IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2421MZ | Recombinant Mouse Anti-Human FZR1 Antibody | ELISA, WB | Mouse antibody |
| VS3-WK1214 | Mouse Anti-FZR1 Recombinant Antibody (VS3-WK1214) | WB | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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