Rabbit Anti-H3.3K27acS31ph Recombinant Antibody (clone 0725W67)
CAT#: VS-0725-FY234
This recombinant antibody, clone 0725W67, targets acetyl-lysine 27 of human Histone H3 sequence, specifically phosphorylated at serine 31. It is validated for Chromatin Immunoprecipitation-Sequencing (ChIP-Seq), Dot Blot (DB), and Western Blot (WB). This unique dual modification is relevant to chromatin structure regulation, with HDAC1 (Histone Deacetylase 1) being a key enzyme in regulating chromatin. This antibody offers a valuable tool for studying complex histone modifications and their roles in gene regulation.
Specifications
- Immunogen
- Acetyl-lysine 27 of human Histone H3 sequence, specifically phosphorylated at serine 31.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human H3.3K27acS31ph
- Species Reactivity
- Human
- Clone
- 0725W67
- Applications
- Chromatin Immunoprecipitation-Sequencing, Dot Blot, Western Blot
- Related Disease
- Cancer
Product Property
- Purification
- Protein A purified
- Molecular Weight
- The molecular weight of the antigen is 17 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 30% glycerol
- Preservative
- 0.035% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Chromatin Immunoprecipitation-Sequencing: 5 µg each
Western Blot: 0.5-2 µg/mL
Dot Blot: 4 µg/mL
Target
- Alternative Names
- H3.3K27acS31ph
- Gene ID
- Long Name
- Histone H3.3 Lysine 27 acetylation Serine 31 phosphorylation (a specific combination of modifications on Histone H3.3)
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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