Mouse Anti-HIV-1 Env Recombinant Antibody (clone 3B3) (CAT#: MRO-2818CQ)
Recombinant mouse antibody to Env. 3B3 bound the Env proteins with high affinity, but did not neutralize any of the tier 1 and tier 2 subtype B and C viruses.
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Cyt
Figure 4 Increased activity of immunotoxins with an aromatic substitution at position 100e of CDR H3.
Env⁺and Env⁻ CHO cells were incubated with various concentrations of the indicated 3B3(scFv)-PE immunotoxins for 48 h and then stained with MTT. The averages for duplicate determinations at 48 h normalized to 100% for no immunotoxin are shown.
Increased Affinity and Stability of an Anti-HIV-1 Envelope Immunotoxin by Structure-based Mutagenesis
FC
Figure 5 FACS analysis of immunotoxin binding to the cell surface.
HeLa cells were infected with vaccinia virus recombinants carrying the indicated HIV-1 env gene or with vector alone.
Increased Affinity and Stability of an Anti-HIV-1 Envelope Immunotoxin by Structure-based Mutagenesis
Cyt
Figure 6 Biochemical stability of immunotoxins.
Freshly purified 3B3(scFv)-PE was incubated in PBS for various intervals, and samples were analyzed by FPLC on a TSK G3000SW column.
Increased Affinity and Stability of an Anti-HIV-1 Envelope Immunotoxin by Structure-based Mutagenesis
Inhib
Figure 7 Inhibition of Env-mediated cell fusion.
HeLa cells were infected with recombinant vaccinia viruses carrying the indicated env gene and a T7 promoter·β-galactosidase gene.
Increased Affinity and Stability of an Anti-HIV-1 Envelope Immunotoxin by Structure-based Mutagenesis
Inhib
Figure 8 Inhibition of spreading infection of PBMC by N31H/Q100eY(scFv)-PE.
PBMC from healthy human donors were infected with HIV-1˪ₐᵥ and incubated in the presence of 0, 3.3, 33, 325, or 3250 ng/ml of N31H/Q100eY(scFv)-PE or with 325 ng/ml of 3B3(scFv)-PEₐₛₚ₅₃₃ for a total of 14 days.
Increased Affinity and Stability of an Anti-HIV-1 Envelope Immunotoxin by Structure-based Mutagenesis
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Recognizes HIV-1 Env
- Species Reactivity
- HIV-1
- Clone
- 3B3
- Applications
- ELISA, WB, SPR, FC, Cyt, Inhib, IF
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The HIV-1 Env antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Western Blot, Surface Plasmon Resonance, Flow Cytometry, Cytotoxicity, Inhibition, Immunofluorescence.
Target
- Alternative Names
- ENV; gp160; envelope glycoprotein; Envelope surface glycoprotein gp160; precursor; hypothetical protein; Envelope surface glycoprotein gp120; Envelope transmembrane domain
- Gene ID
- 155971
- UniProt ID
- P04578
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| NABG-057 | Recombinant Anti-HIV-1 env VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# MRO-2818CQ, RRID: AB_3111539)
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