Human Anti-HIV-1 Env Recombinant Antibody (clone PGT151)

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Citations

1. Yamamoto, Mizuki, et al. "Cell-cell and virus-cell fusion assay-based analyses of alanine insertion mutants in the distal α9 portion of the JRFL gp41 subunit from HIV-1." Journal of Biological Chemistry 294.14 (2019): 5677-5687. https://doi.org/10.1074/jbc.RA118.004579
   This research investigates alanine insertion mutations in the distal portion of the α9 helix in the gp41 subunit of HIV-1 envelope protein (Env). The study focuses on six alanine insertion mutants in the R5-tropic JRFL Env, specifically targeting positions 641-646 where several glutamine residues are conserved. The researchers found that the 644A mutant showed the most severe defect in syncytia formation, with decreased fusion pore activity observed in all mutants except 641A. Through sequence analysis and substitution experiments, they demonstrated that the glutamine residue at position 644, which forms complex hydrogen-bond networks with other polar residues on the six-helix bundle surface, is critical for cell-cell fusion. Additionally, the team developed a split NanoLuc reporter-based assay specifically for the virus-cell membrane fusion step, revealing that even syncytia-competent mutants failed to display NanoLuc activities, suggesting differences between cell-cell and virus-cell fusion mechanisms.
   Creative Biolabs provided the anti-HIV-1 Env neutralizing antibody (clone PGT151), which was used to validate the specificity of their newly developed R-BiT (Vpr-HiBiT) assay for membrane fusion. This antibody, along with other inhibitors, helped confirm that the novel assay could faithfully monitor the membrane fusion step of viral infection independent of downstream replication steps such as reverse transcription and integration. The use of PGT151 was instrumental in demonstrating the technical reliability of their fusion-specific assay, enabling the researchers to make more accurate distinctions between the fusion capabilities of wild-type and mutant Env proteins.

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(Creative Biolabs Cat# PABL-153, RRID: AB_3111630)

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Publications

Our products have been used extensively by scientists all over the world to accelerate their life science research. Here, we present a collection of publications that cite this product.

Yamamoto, Mizuki, et al. "Cell–cell and virus–cell fusion assay–based analyses of alanine insertion mutants in the distal α9 portion of the JRFL gp41 subunit from HIV-1." Journal of Biological Chemistry 294.14 (2019): 5677-5687.

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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