Mouse Anti-HIV-1 Nef Recombinant Antibody (clone F1) (CAT#: MRO-4228CQ)
Recombinant mouse antibody to Nef(148-157). This antibody recognized Nef epitopes located at amino acid residues 148-157.
Specific Inquiry
FC
Figure 1 Jurkat E6.1 cells were infected with lentiviral vectors encoding EGFP fused to the Nef proteins.
A representative histogram from three independent experiments is shown.
Johnson, A. L., Dirk, B. S., Coutu, M., Haeryfar, S. M., Arts, E. J., Finzi, A., & Dikeakos, J. D. (2016). A highly conserved residue in HIV-1 Nef alpha helix 2 modulates protein expression. mSphere, 1(6), e00288-16.
WB
Figure 2 Uninfected (UI) Jurkat E6.1 cells or cells infected with the lentiviral vectors described above expressing nonfusion Nef proteins were collected and lysed at 48 h postinfection.
Lysates were analyzed for Nef, EGFP, and actin protein levels by Western blotting.
Johnson, A. L., Dirk, B. S., Coutu, M., Haeryfar, S. M., Arts, E. J., Finzi, A., & Dikeakos, J. D. (2016). A highly conserved residue in HIV-1 Nef alpha helix 2 modulates protein expression. mSphere, 1(6), e00288-16.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgM
- Specificity
- Recognizes HIV-1 Nef(148-157)
- Species Reactivity
- HIV-1
- Clone
- F1
- Applications
- WB, FC
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The HIV-1 Nef antibody has been reported in applications of Western Blot, Flow Cytometry.
Target
- Alternative Names
- HIV Nef; Human Immunodeficiency Viruse Nef; Human Immunodeficiency Viruse; HIV Nef protein; HIV Nef-protein; p27; HIV1gp9; F-protein; Negative factor
- Gene ID
- 156110
- UniProt ID
- P04601
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-013 | Recombinant Anti-HIV nef VHH Single Domain Antibody | WB, ELISA, IP, FuncS | Llama VHH |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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