Human Anti-LF Recombinant Antibody (clone LF11H) (CAT#: FAMAB-0616WJ)

Figure 1 ELISA titration of anti-LF MAbs.

Recombinant LF was used to coat ELISA plates. Wells were then incubated with various dilutions of LF9D, LF10E, and LF11H IgGs, and the bound IgGs were detected by the addition of peroxidase-conjugated anti-human Fc antibody followed by tetramethylbenzidine substrate. OD450, optical density at 450 nm.

Chen, Z., Moayeri, M., Crown, D., Emerson, S., Gorshkova, I., Schuck, P., ... & Purcell, R. H. (2009). Novel chimpanzee/human monoclonal antibodies that neutralize anthrax lethal factor, and evidence for possible synergy with anti-protective antigen antibody. Infection and immunity, 77(9), 3902-3908.

Figure 2 LF10E and LF11H neutralize LT toxicity.

LT (100 ng/ml) was incubated with serial dilutions of each antibody (1 h, 37°C) prior to treatment of RAW264.7 macrophage cells with LT-MAb mixtures for 4 h. Cell viability was assessed by 3-(4,5-dimethylthiazo-2-yl)-2,5-diphenyltetrazolium bromide staining and is presented as a percentage of the value for untreated controls.

Chen, Z., Moayeri, M., Crown, D., Emerson, S., Gorshkova, I., Schuck, P., ... & Purcell, R. H. (2009). Novel chimpanzee/human monoclonal antibodies that neutralize anthrax lethal factor, and evidence for possible synergy with anti-protective antigen antibody. Infection and immunity, 77(9), 3902-3908.

Figure 3 LF10E and LF11H bind to LF amino acids 1 to 254 and prevent LT-mediated MEK cleavage in cells but do not prevent LF binding to cleaved PA.

Purified toxin proteins LF, PA, and EF and fusion proteins FP59 and FP119 were loaded on SDS-polyacrylamide gels (2 ng/well for panel A and 10 ng/well for panel B) and probed with LF10E (A) or LF11H (B) by Western blotting. LT (1 μg/ml) was preincubated with MAbs (100 μg/ml) for 1 h prior to addition of LT or LT-MAb mixtures to CHO cells (C) or RAW 264.7 cells (D). Toxins were then allowed to bind to cells for 1 h prior to Western blotting as described in Materials and Methods. No treatment (NT) was used as a negative control.

Chen, Z., Moayeri, M., Crown, D., Emerson, S., Gorshkova, I., Schuck, P., ... & Purcell, R. H. (2009). Novel chimpanzee/human monoclonal antibodies that neutralize anthrax lethal factor, and evidence for possible synergy with anti-protective antigen antibody. Infection and immunity, 77(9), 3902-3908.