Mouse Anti-MAP3K2 Monoclonal Antibody (VS7-0425-WR343)
CAT#: VS7-0425-WR343
This product is a mouse IgG antibody that binds to human MAP3K2. The antibody can be used for WB, IF, ICC, and ELISA assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21540/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human gallbladder
Immunohistochemical staining of human gallbladder shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21540/48792_A_5_4_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21540/48792_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21540/48792_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21540/48792_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21540/48792_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21540/48792_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169967-MAP3K2
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Specifications
- Immunogen
- Recombinant human MAP3K2 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human MAP3K2
- Species Reactivity
- Human
- Molecule Class
- Serine/Threonine Protein Kinase
- Applications
- WB, IF, ICC, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunofluorescence: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- MEKK2; MEKK2B
- Gene ID
- 10746
- UniProt ID
- Q9Y2U5
- Long Name
- Mitogen-Activated Protein Kinase Kinase Kinase 2
- Cellular Localization
- Cytoplasm; Nucleus
- Post Translation Modifications
- Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001358839.1
- Function
- Kinase; Serine/threonine-protein kinase; Transferase
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Related Diseases
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "MAP3K2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2139 | Hi-Affi™ Recombinant Rabbit Anti-MAP3K2 Monoclonal Antibody (DS2139AB) | FC, ICC, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0985-CN | Recombinant Rabbit Anti-MAP3K2 Monoclonal Antibody (CBACN-364) | WB, IF, IHC, IP, FC | Rabbit IgG |
| ZG-0276C | Mouse Anti-MAP3K2 Recombinant Antibody (ZG-0276C) | WB, IF, ELISA | Mouse IgG |
| VS3-XY1031 | Mouse Anti-MAP3K2 Recombinant Antibody (clone 4B4) | ELISA, WB, ICC | Mouse IgG1 |
| VS3-FY881 | Recombinant Rabbit Anti-MAP3K2 Antibody (clone R06-2K7) | WB, ICC, IF, IP | Rabbit IgG |
| VS-0724-FY10 | Rabbit Anti-MAP3K2 Recombinant Antibody (clone E6) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4217 | Anti-MAP3K2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4218 | Anti-Mouse MAP3K2 Immunohistochemistry Kit | IHC |
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