Human Anti-NEDD8-CUL1^WHB Recombinant Antibody (clone N8C_Fab3b) (CAT#: VS-0624-FY22)

• Host Species
• Human

• Type
• Human IgG

• Specificity
• Human NEDD8-CUL1^WHB

• Species Reactivity
• Human

• Clone
• N8C_Fab3b

• Applications
• Flow Cytometry, Western Blot, ELISA, Immunoprecipitation

• Related Disease
• Malignant Astrocytoma and Machado-Joseph Disease

• Clonality
• Monoclonal Antibody

• Purification
• Protein A purified

• Purity
• >95% as determined by SDS-PAGE

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• PBS, pH 7.4

• Preservative
• No preservatives

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Alternative Names
• Neural Precursor Cell Expressed, Developmentally Down-Regulated 8; Ubiquitin-Like Protein Nedd8; Neddylin; NEDD-8; Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8; NEDD8-CUL1^WHB

• Gene ID
• 4738

• UniProt ID
• Q15843

• Long Name
• Neural Precursor Cell Expressed, Developmentally Down-Regulated 8

• Sequence Similarities
• Belongs to the ubiquitin family.

• Cellular Localization
• Nucleus

• Post Translation Modifications
• Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.4 Publications, (Microbial infection) Deamidated at Gln-40 by bacterial cyclomodulin Cif produced by enteropathogenic E.coli, Y.pseudotuberculosis or B.pseudomallei, leading to impair NEDD8 ability to activate cullin-RING-based E3 ubiquitin-protein ligase complexes (CRL complexes) (PubMed:20688984, PubMed:21903097, PubMed:23589306, PubMed:26632597, PubMed:23175788)., Deamidation occurs on NEDD8-modified cullins (PubMed:20850415, PubMed:21903097)., NEDD8 deamidation prevents switching from the inactive to active state by maintaining the 'closed' structure of the CRL complexes (PubMed:23589306, PubMed:26632597)., Deamidation may also impair its deconjugation by the COP9 signalosome; However this result needs additional evidences (PubMed:20850415, PubMed:21903097).

• Protein Refseq
• NP_006147.1

• Function
• Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis via its conjugation to a limited number of cellular proteins, such as cullins or p53/TP53 (PubMed:9694792, PubMed:10318914, PubMed:10597293, PubMed:11953428, PubMed:15242646, PubMed:14690597).
  Attachment of NEDD8 to cullins is critical for the recruitment of E2 to the cullin-RING-based E3 ubiquitin-protein ligase complex, thus facilitating polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins (PubMed:9694792, PubMed:10318914, PubMed:10597293, PubMed:11953428, PubMed:20688984).
  Attachment of NEDD8 to p53/TP53 inhibits p53/TP53 transcriptional activity (PubMed:15242646).
  Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M (PubMed:14690597).

Secondary Antibody

• 2ND-024CQ
• Goat Anti-Human IgG (H&L) Antibody

Isotype Control

• MRO-A1587-YJ
• Human IgG Isotype control, Polyclonal

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