Human Anti-NEDD8-CUL1WHB Recombinant Antibody (clone N8C_Fab3b)
CAT#: VS-0624-FY22
This is a human monoclonal antibody that binds to NEDD8-CUL1 WHB. CUL1's WHB domain is flexibly tethered to its connecting helix-29. N8C_Fab3b recognizes a unique interface spanning both NEDD8 and CUL1. N8C_Fab3b binds a specific arrangement of NEDD8 and its linked CUL1WHB domain. N8C_Fab3b inhibited UBE2D- and neddylated CRL1BTRC-dependent ubiquitylation of a peptide substrate derived from phospho-NFKBIA.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27583/1762_H7_3_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60254/1123_C6_2_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/624_G8_1_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/ihc_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/12097_B_8_5.jpg
Normal Tissue
Figure 6 Parathyroid gland
Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/12097_A_3_8.jpg
Normal Tissue
Figure 7 Colon
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/12097_A_9_3.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/12097_A_8_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/12097_A_5_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4082/12097_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000129559-NEDD8
❮
❯
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human NEDD8-CUL1WHB
- Species Reactivity
- Human
- Clone
- N8C_Fab3b
- Applications
- Flow Cytometry, Western Blot, ELISA, Immunoprecipitation
- Related Disease
- Malignant Astrocytoma and Machado-Joseph Disease
Product Property
- Clonality
- Monoclonal Antibody
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Neural Precursor Cell Expressed, Developmentally Down-Regulated 8; Ubiquitin-Like Protein Nedd8; Neddylin; NEDD-8; Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8; NEDD8-CUL1WHB
- Gene ID
- 4738
- UniProt ID
- Q15843
- Long Name
- Neural Precursor Cell Expressed, Developmentally Down-Regulated 8
- Sequence Similarities
- Belongs to the ubiquitin family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.4 Publications, (Microbial infection) Deamidated at Gln-40 by bacterial cyclomodulin Cif produced by enteropathogenic E.coli, Y.pseudotuberculosis or B.pseudomallei, leading to impair NEDD8 ability to activate cullin-RING-based E3 ubiquitin-protein ligase complexes (CRL complexes) (PubMed:20688984, PubMed:21903097, PubMed:23589306, PubMed:26632597, PubMed:23175788)., Deamidation occurs on NEDD8-modified cullins (PubMed:20850415, PubMed:21903097)., NEDD8 deamidation prevents switching from the inactive to active state by maintaining the 'closed' structure of the CRL complexes (PubMed:23589306, PubMed:26632597)., Deamidation may also impair its deconjugation by the COP9 signalosome; However this result needs additional evidences (PubMed:20850415, PubMed:21903097).
- Protein Refseq
- NP_006147.1
- Function
- Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis via its conjugation to a limited number of cellular proteins, such as cullins or p53/TP53 (PubMed:9694792, PubMed:10318914, PubMed:10597293, PubMed:11953428, PubMed:15242646, PubMed:14690597).
Attachment of NEDD8 to cullins is critical for the recruitment of E2 to the cullin-RING-based E3 ubiquitin-protein ligase complex, thus facilitating polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins (PubMed:9694792, PubMed:10318914, PubMed:10597293, PubMed:11953428, PubMed:20688984).
Attachment of NEDD8 to p53/TP53 inhibits p53/TP53 transcriptional activity (PubMed:15242646).
Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M (PubMed:14690597).
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-0624-FY22. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "NEDD8"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-945-VHH | Recombinant Anti-human NEDD8 VHH Single Domain Antibody | IHC, ICC, IP, ChiP, Neut | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2409 | Hi-Affi™ Recombinant Rabbit Anti-NEDD8 Monoclonal Antibody (DS2409AB) | ICC, IF, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1072-CN | Recombinant Rabbit Anti-NEDD8 Monoclonal Antibody (JF69-09) | WB, IF, IHC, IP | Rabbit IgG |
| VS3-WK435 | Rabbit Anti-NEDD8 Recombinant Antibody (clone JF69-09) | WB, ICC, IF, IHC, IP | Rabbit IgG |
| VS3-XY1147 | Mouse Anti-NEDD8 Recombinant Antibody (clone 1A7) | ELISA, WB, IHC, FC, ICC | Mouse IgG1 |
| VS3-FY1635 | Rabbit Anti-NEDD8 Recombinant Antibody (clone R02-4G8) | WB, IHC-Fr, IHC-P, IF, IP | Rabbit IgG |
| VS7-0425-WR460 | Mouse Anti-NEDD8 Recombinant Antibody (clone 5B8) | WB, IHC, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY338 | Rabbit Anti-NHP NEDD8 Recombinant Antibody (clone B.226.2) | WB, IHC, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1477 | Anti-NEDD8 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4769 | Anti-Human NEDD8 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: ELISA, IP, WB, IHC, IF, FuncS
Application: ELISA, FC, WB, Block
Application: WB, ELISA, IF, FuncS, Apop, BA
Application: Neut, FC, IHC-Fr, IP, BA
-3.jpg)
Application: Neut
-3.jpg)
Application: ELISA, WB
Application: ELISA, Block, WB, FC, IP
Application: WB, IHC-Fr, IHC-P, ICC, IF
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
