Mouse Anti-NHP DOK2 Recombinant Antibody (clone OTI2D10) (CAT#: VS-1024-XY171)
This mouse-derived monoclonal antibody is designed for the detection of DOK2, with reactivity across dog, human, mouse, non-human primate, and rat tissues. The immunogen is a full-length recombinant human DOK2 protein, ensuring high specificity and comprehensive reactivity. Primarily validated for Western blot (WB) applications, this antibody provides robust performance for the detailed analysis of DOK2 expression and offers valuable insights into its role across multiple species.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5507/1868_A2_32_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5507/1868_A2_32_selected.jpg
Figure 2 High expression in lymph node
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/66571/161486_A_7_8_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/66571/161486_A_7_8_val_selected.jpg
Figure 3 Low expression in skeletal muscle
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/66571/161486_B_2_6_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/66571/161486_B_2_6_val_selected.jpg
Figure 4 Lung
(Alveolar cells type Alveolar cells type I Macrophages Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4379/13249_A_3_4.jpg
(Alveolar cells type Alveolar cells type I Macrophages Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4379/13249_A_3_4.jpg
Figure 5 Lymph node
(Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4379/13249_A_9_8.jpg
(Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4379/13249_A_9_8.jpg
Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, HEL, HMC-1, JURKAT, THP-1, U-937)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000147443-DOK2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000147443-DOK2
Specifications
- Immunogen
- Full length human recombinant protein of DOK2
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- DOK2
- Species Reactivity
- Dog, Human, Mouse, Non-human primate, Rat
- Clone
- OTI2D10
- Applications
- WB
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 1% BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:200-1:500
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1035 | Hi-Affi™ Rabbit Anti-DOK2 Recombinant Antibody (clone DS1035AB) | ELISA | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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