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    Mouse Anti-NHP HSPA9 Recombinant Antibody (clone 30A5) (CAT#: VS-1024-XY265)

    Optimized for detecting HSPA9, this mouse monoclonal antibody demonstrates broad reactivity across guinea pig, hamster, human, mouse, non-human primate, sheep, pig, rabbit, and Xenopus. With its proven effectiveness in Western blot (WB) and immunofluorescence (IF), this antibody is an invaluable tool for elucidating HSPA9 expression and localization in a wide range of species, facilitating diverse research and diagnostic applications.

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    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
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    Conjugation :
    Fc Engineering :
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    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/if_selected.jpg
    Figure 2 IF staining of human cell line U-251 MG
    Figure 2 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5219/if_selected.jpg
    Figure 3 IHC staining of human cerebral cortex
    Figure 3 IHC staining of human cerebral cortex
    (Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/ihc_selected.jpg
    Figure 4 IHC staining of human kidney
    Figure 4 IHC staining of human kidney
    (Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5219/ihc_selected.jpg
    Figure 5 IF staining of human cell line A431
    Figure 5 IF staining of human cell line A431
    (Immunofluorescent staining of human cell line A431 shows localization to mitochondria.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5219/639_A10_1_blue_red_green.jpg
    Normal Tissue
    Figure 6 Cerebral cortex
    Figure 6 Cerebral cortex
    (Endothelial cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Glial cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous
    Neuronal cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Neuropil
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_B_9_5.jpg
    Figure 7 Colon
    Figure 7 Colon
    (Glandular cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Peripheral nerve/ganglion
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_9_3.jpg
    Figure 8 Liver
    Figure 8 Liver
    (Cholangiocytes
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous
    Hepatocytes
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_8_4.jpg
    Figure 9 Kidney
    Figure 9 Kidney
    (Cells in glomeruli
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous
    Cells in tubules
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_9_5.jpg
    Figure 10 Testis
    Figure 10 Testis
    (Leydig cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Pachytene spermatocytes
    Staining:Medium
    Intensity: Strong
    Quantity: <25%
    Sertoli cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Spermatogonia cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_6_6.jpg
    Figure 11 Lymph node
    Figure 11 Lymph node
    (Germinal center cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Non-germinal center cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_7_8.jpg
    RNA Expression
    Figure 12 RNA cell line category: Low cell line specificity
    Figure 12 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000113013-HSPA9

    Specifications

    • Immunogen
    • Grp75
    • Host Species
    • Mouse
    • Type
    • Mouse IgG1
    • Specificity
    • HSPA9
    • Species Reactivity
    • Guinea pig, Hamster, Human, Mouse, Non-human primate, Sheep, Pig, Rabbit, Xenopus
    • Clone
    • 30A5
    • Applications
    • WB, IF

    Product Property

    • Purification
    • Protein A purified
    • Purity
    • >95%, determined by SDS-PAGE
    • Format
    • Liquid
    • Buffer
    • PBS, pH 7.4, with 0.1 mM PMSF, 50% glycerol
    • Storage
    • Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

    Applications

    • Application Notes
    • WB: 1:3,000, IF: 1:100

    Target

    • Alternative Names
    • Heat Shock Protein Family A (Hsp70) Member 9; Heat Shock 70kDa Protein 9 (Mortalin); 75 KDa Glucose-Regulated Protein; Peptide-Binding Protein 74; Mortalin2; Mortalin; HSPA9B; GRP-75; GRP75; PBP74; MOT; Epididymis Secretory Sperm Binding Protein Li 124m; Heat Shock 70kDa Protein 9B (Mortalin-2); Catecholamine-Regulated Protein 40; Stress-70 Protein; Mitochondrial;

    Recommended Products

    Recommended Dilution Buffer

    Isotype Control

    Secondary Antibody

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

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