Rabbit Anti-HSPA9 Recombinant Antibody (VS13-YC558)
CAT#: VS13-YC558
The Rabbit Anti-HSPA9 Recombinant Antibody is specific for human, mouse and rat HSPA9. HSPA9 is a chaperone of the heat shock protein 70 family assisting in protein folding and ATPase activity, with HSPA9 primarily localized to mitochondria but present in other cellular components. The anti-HSPA9 antibody can be used for Western blot (1/500-1/1000).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5219/if_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5219/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 5 IF staining of human cell line A431
Immunofluorescent staining of human cell line A431 shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5219/639_A10_1_blue_red_green.jpg
Normal Tissue
Figure 6 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_B_9_5.jpg
Normal Tissue
Figure 7 Colon
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_9_3.jpg
Normal Tissue
Figure 8 Liver
Cholangiocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_8_4.jpg
Normal Tissue
Figure 9 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_9_5.jpg
Normal Tissue
Figure 10 Testis
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Strong
Quantity: <25%
Sertoli cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_6_6.jpg
Normal Tissue
Figure 11 Lymph node
Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_7_8.jpg
RNA Expression
Figure 12 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000113013-HSPA9
❮
❯
Specifications
- Immunogen
- Recombinant human Grp75.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat HSPA9
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 150 mM NaCl and 50% Glycerol.
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Target
- Alternative Names
- Heat Shock Protein Family A (Hsp70) Member 9; Heat Shock 70kDa Protein 9 (Mortalin); 75 KDa Glucose-Regulated Protein; Peptide-Binding Protein 74; Mortalin2; Mortalin; HSPA9B; GRP-75; GRP75; PBP74; MOT; Epididymis Secretory Sperm Binding Protein Li 124m; Heat Shock 70kDa Protein 9B (Mortalin-2); Catecholamine-Regulated Protein 40; Stress-70 Protein, Mitochondrial;
- Gene ID
- 3313
- UniProt ID
- P38646
- Long Name
- Heat Shock Protein Family A (Hsp70) Member 9
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "HSPA9"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1346-sdAb | Recombinant Anti-Human HSPA9 VHH Single Domain Antibody | WB, IP, ICC, FC, FA | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1698 | Rabbit Anti-HSPA9 Recombinant Antibody (clone DS1698AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0698-CN | Mouse Anti-HSPA9 Recombinant Antibody (clone A6-A5-B1) | WB, IF, IHC, FC | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1908-CN | Rabbit Anti-HSPA9 Polyclonal Antibody (MRO-1908-CN) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-1909-CN | Rabbit Anti-HSPA9 Polyclonal Antibody (MRO-1909-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS7-0425-WR95 | Mouse Anti-HSPA9 Recombinant Antibody (clone 8D2D11) | WB, IHC, ICC, FC | Mouse IgG2a |
| VS7-0425-WR96 | Mouse Anti-HSPA9 Recombinant Antibody (clone 3A12G3) | WB, IHC, ICC, FC | Mouse IgG1 |
| VS7-0425-WR97 | Mouse Anti-HSPA9 Recombinant Antibody (clone 7E2E4) | WB, IHC, ICC, FC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY265 | Mouse Anti-NHP HSPA9 Recombinant Antibody (clone 30A5) | WB, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1075 | Anti-HSPA9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3332 | Anti-Mouse HSPA9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3331 | Anti-Human HSPA9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3333 | Anti-Rat HSPA9 Immunohistochemistry Kit | IHC |
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