Human Anti-PBMC Recombinant Antibody (clone AG11) (CAT#: FAMAB-0093-CN)

This product is a human antibody that recognizes PBMC. The antibody AG11 can be used for immunoassay techniques such as: ELISA, FC.


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ELISA

Figure 1 Binding inhibition ELISA assay of Mabs.

Figure 1 Binding inhibition ELISA assay of Mabs.

Blinding inhibition of biotinylated EH1 mAb by unlabeled AG11 (8) and AE6 (R) mAbs. F14.11 (a) was a control mAb.

IF

Figure 2 Construction and expression of intracellular ScFv tagged with a GFP reporter.

Figure 2 Construction and expression of intracellular ScFv tagged with a GFP reporter.

HEK 293 cells transfected with pDEF-GFP or pDEF-ScFv-GFP, and expressing: A: AG11 ScFv tagged with GFP (phase). B: AG11 ScFv tagged with GFP (£uorescence). tagged with GFP (£uorescence). Magni¢cation, X36.

IP

Figure 3 Intracellularly expressed ScFv is immunoprecipitated by immobilized recombinant Nef-GST protein.

Figure 3 Intracellularly expressed ScFv is immunoprecipitated by immobilized recombinant Nef-GST protein.

A: Immunoprecipitation of ScFv with Nef-GST cross-linked to Sepharose beads from cells transfected with the control vector (1), pDEF-GFP (2), pDEF- (AG11)ScFv-GFP (3), and pDEF- (EH1)ScFv-GFP (4). B: Immunoblot of the total cell lysate of the cells transfected with control vector (1) and pDEF-GFP (2). C: Immunoprecipitation of cells expressing the control vector (1) and pDEF-GFP (2) corresponding to B, by Nef-GST cross-linked with Sepharose beads.


Specifications

  • Immunogen
  • Human peripheral blood leukocytes (PBMC)
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • Human IgM
  • Specificity
  • Human PBMC
  • Species Reactivity
  • Human
  • Clone
  • AG11
  • Applications
  • ELISA, FC

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • PBMC

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "Clone AG11"

See other products for "PBMC"

Single-domain Antibody

CAT Product Name Application Type
HPAB-0207-FY Recombinant llama Anti-PBMC Antibody (HPAB-0207-FY) E llama VHH

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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