Rabbit Anti-Phospho-MAPK3 (T202) + MAPK1 (T185) Recombinant Antibody (clone 2D5) (CAT#: ZG-0456U)

Figure 1 Rabbit Anti-Phospho-MAPK3 (T202) + MAPK1 (T185) Antibody (ZG-0456U) in WB

Western Blot
Positive WB detected in A549 whole cell lysate, HepG2 whole cell lysate(treated with EGF or not)
All lanes Phospho-MAPK3 antibody at 2.35μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa

Figure 2 Rabbit Anti-Phospho-MAPK3 (T202) + MAPK1 (T185) Antibody (ZG-0456U) in IHC

IHC image of ZG-0456U diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Figure 3 Rabbit Anti-Phospho-MAPK3 (T202) + MAPK1 (T185) Antibody (ZG-0456U) in IF

Immunofluorescence staining of Hela cells with ZG-0456U at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).