Rabbit Anti-Phospho-MAPK3 (T202) + MAPK1 (T185) Recombinant Antibody (clone 2D5) (CAT#: ZG-0456U)
Rabbit Anti-Phospho-MAPK3 (T202) + MAPK1 (T185) Antibody (clone 2D5) displayed high specificity against the MAPK1/MAPK3 protein of Human.
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WB
Figure 1 Rabbit Anti-Phospho-MAPK3 (T202) + MAPK1 (T185) Antibody (ZG-0456U) in WB
Western Blot
Positive WB detected in A549 whole cell lysate, HepG2 whole cell lysate(treated with EGF or not)
All lanes Phospho-MAPK3 antibody at 2.35μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
IHC
Figure 2 Rabbit Anti-Phospho-MAPK3 (T202) + MAPK1 (T185) Antibody (ZG-0456U) in IHC
IHC image of ZG-0456U diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IF
Figure 3 Rabbit Anti-Phospho-MAPK3 (T202) + MAPK1 (T185) Antibody (ZG-0456U) in IF
Immunofluorescence staining of Hela cells with ZG-0456U at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-MAPK3 (T202) + MAPK1 (T185)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human Phospho-MAPK3 (T202) + MAPK1 (T185)
- Species Reactivity
- Human
- Clone
- 2D5
- Applications
- ELISA, WB, IHC, IF
- Conjugate
- Unconjugated
- Related Disease
- Noonan Syndrome 13, Specific Learning Disability, Bile Duct Cancer and Pancreatic Adenocarcinoma.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200
Target
- Alternative Names
- ERK; p38; p40; p41; ERK2; ERT1; NS13; ERK-2; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; p42-MAPK; mitogen-activated protein kinase 1; MAP kinase 1; MAP kinase 2; MAPK 2; extracellular signal-regulated kinase 2; mitogen-activated protein kinase 2; protein tyrosine kinase ERK2; EC 2.7.11.24; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK; mitogen-activated protein kinase 3; MAPK 1; extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; insulin-stimulated MAP2 kinase; microtubule-associated protein 2 kinase
Related Resources
Maturity Onset Diabetes of the Young
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0546-CN | Recombinant Rabbit Anti-MAPK1/MAPK3 Monoclonal Antibody (CBACN-217) | WB, IF, IP, FC | Rabbit IgG |
| MRO-2323-CN | Recombinant Rabbit Anti-MAPK1/MAPK3 (phosphorylated Thr202/Tyr204+Thr185/Tyr187) Monoclonal Antibody (CBACN-614) | WB, IP | Rabbit IgG |
| VS3-WK2063 | Rabbit Anti-MAPK1 (pT202)/MAPK3 (pT185) Recombinant Antibody (clone R49-1Y-4) | WB, IHC-P, ICC, IF, IP | Rabbit IgG |
| VS3-WK2094 | Rabbit Anti-MAPK1 (pT202/pY204)/MAPK3 (pT185/pT187) Recombinant Antibody (clone R08-8S3) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
| VS3-WK2190 | Mouse Anti-MAPK1/MAPK3 (pY222/pY205) Recombinant Antibody (VS3-WK2190) | IHC-P, IF | Mouse IgG |
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