Mouse Anti-PPIB Recombinant Antibody (VS3-CJ793)
CAT#: VS3-CJ793
This product is a mouse antibody that recognizes human, mouse, and rat endogenous levels of Cyclophilin B protein.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HUVEC TERT2
Immunofluorescent staining of human cell line HUVEC TERT2 shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12720/1752_C1_2_cr5804bfa7d57a4_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11487/30837_B_9_5.jpg
Normal Tissue
Figure 3 Thyroid gland
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11487/30837_B_1_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Enterocytes
Staining: Low
Intensity: Weak
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11487/30837_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11487/30837_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11487/30837_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11487/30837_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11487/30837_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166794-PPIB/subcellular
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Specifications
- Immunogen
- KLH-conjugated synthetic peptide encompassing a sequence of human Cyclophilin B
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, Mouse, Rat endogenous levels of Cyclophilin B protein
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC
- Conjugate
- Unconjugated
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 30% Glycerol, 0.87% NaCl, 0.42% Potassium phosphate, pH7.3.
- Preservative
- 0.01% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipped at 4°C
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:1000-1:2000), Immunohistochemistry (1:50-1:200).
Target
- Alternative Names
- OI9; CYPB; SCYLP; CYP-S1; HEL-S-39
- Gene ID
- 5479
- UniProt ID
- P23284
- Sequence Similarities
- Belongs to the cyclophilin-type PPIase family. PPIase B subfamily.
- Cellular Localization
- Endoplasmic reticulum, Virion
- Protein Refseq
- NP_000933.1
- Function
- PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3523z | Mouse Anti-PPIB Recombinant Antibody (clone 34B5) | WB, IP, IF, IHC, ELISA | Mouse IgG2a, κ |
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| VS3-FY1174 | Recombinant Rabbit Anti-PPIB Antibody (clone R01-2F8) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2805 | Hi-Affi™ Recombinant Rabbit Anti-PPIB Monoclonal Antibody (DS2805AB) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1427 | AbPlus™ Anti-PPIB Magnetic Beads (VS-0724-YC1427) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1709 | Anti-PPIB Immunohistochemistry Kit | IHC | |
| VS-0525-XY5596 | Anti-Human PPIB Immunohistochemistry Kit | IHC |
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