Human Anti-SMARCA4 Recombinant Antibody (ZG-433C)
CAT#: ZG-433C
This product is a human antibody that recognizes SMARCA4. It can be used for ELISA and IP.
Tested Data
Gene Expression
ELISA
Figure 1 Human Anti-SMARCA4 Antibody (ZG-433C) in ELISA
Antibody was tested on human SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a4 isoform B (in blue) and non-target protein (in red).
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli fibrillar center.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48340/831_G7_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4208/if_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human colon
Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4208/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nucleoli fibrillar center.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4208/659_G5_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4208/15743_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4208/15743_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4208/15743_A_9_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4208/15743_A_8_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Pachytene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4208/15743_A_4_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4208/15743_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000127616-SMARCA4
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Specifications
- Immunogen
- SWI/SNF-related matrix-associated actin-dependent regulator ofchromatin a4 isoform B. Domain: 1451-1580 of 1647, bromodomain.
- Host Species
- Human
- Type
- Human IgG1, κ
- Specificity
- Human SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a4 isoform B
- Species Reactivity
- Human
- Applications
- ELISA, IP
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as deterimined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- ELISA 1: 1000 - 1: 5000. IP 2 µg/ml
Target
- Alternative Names
- SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily A, Member 4; Mitotic Growth And Transcription Activator; ATP-Dependent Helicase SMARCA4; Global Transcription Activator Homologous Sequence; Sucrose Nonfermenting-Like 4; BRG1-Associated Factor 190A; Protein Brahma Homolog 1; BRM/SWI2-Related Gene 1; Homeotic Gene Regulator; Brahma Protein-Like 1; Nuclear Protein GRB1; Protein BRG-1; SNF2-Like 4; SNF2-Beta; BAF190A; SNF2L4;
- Gene ID
- 6597
- UniProt ID
- P51532
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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