Rabbit Anti-TriMethyl-Histone H3-K36 Recombinant Antibody (clone ARC50050)
CAT#: VS-0725-FY241
The Rabbit Anti-TriMethyl-Histone H3-K36 Recombinant Antibody (clone ARC50050) targets a synthetic trimethylated peptide centered around K36 of human Histone H3. It is suitable for Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Sequencing (ChIP-seq), Enzyme-linked Immunosorbent Assay (ELISA), and Dot Blot (DB). This antibody is a valuable tool for studying histone methylation, a key post-translational modification crucial for transcription regulation, DNA repair, replication, and chromosomal stability.
Specifications
- Immunogen
- Human Histone H3 (NP_003520.1): A synthetic trimethylated peptide centered around K36.
Sequence: MART KQTA RKST GGKA PRKQ LATK AARK SAPA TGGV KKPH RYRP GTVA LREI RRYQ KSTE LLIR KLPF QRLV REIA QDFK TDLR FQSS AVMA LQEA CEAY.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TriMethyl-Histone H3-K36
- Species Reactivity
- Human, Mouse, Rat, Other (Wide Range Predicted)
- Clone
- ARC50050
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation-Sequencing, Enzyme-linked Immunosorbent Assay, Dot Blot
- Related Disease
- Cancer
Product Property
- Purification
- Affinity purified
- Molecular Weight
- The calculated MW is 15 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS, 0.05% BSA, 50% glycerol, pH7.3
- Preservative
- 0.05% Proclin300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Dot Blot: 1:500-1:1000
Western Blot: 1:500-1:1000
Immunoprecipitation: 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Immunohistochemistry-Paraffin: 1:100-1:500
Chromatin Immunoprecipitation: 5μg antibody
Chromatin Immunoprecipitation-Sequencing: 1:50-1:100
Target
- Alternative Names
- H3.4; H3/g; H3FT; H3t; HIST3H3; Histone H3; HIST1H3A; TriMethyl-Histone H3-K36
- Gene ID
- 8290
- Long Name
- Trimethylated Histone H3 at Lysine 36
- Cellular Localization
- Chromosome, nucleus
- Post Translation Modifications
- Methylation
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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